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Cell No. : Cell Name
RCB0495 : KU812  update : 2022/03/16
CommentChronic myelogeneous leukemia, Ph1 chromosome (+), basophil-like cells.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mizusawa, Hiroshi
Originator Kishi, Kenji
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Classification cancer
History JCRB0104
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0379
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 50-108(50) : /58(1),59(4),60(8),61(5),62(5),63(9)/
STR(human) OK
deposit info
lot info
Reference information Reference 6
User's Publication 16

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1796  Valent P, Besemer J, Sillaber C, Butterfield, J H, Eher R, Majdic O, Kishi K, Klepetko W, Eckersberger F, Lechner K, et al.  Failure to detect IL-3-binding sites on human mast cells.  J Immunol  1990  145:3432-7  PubMed ID: 2230127  
1795  Nakazawa M, Mitjavila, M T, Debili N, Casadevall N, Mayeux P, Rouyer-Fessard P, Dubart A, Romeo, P H, Beuzard Y, Kishi K, et al.  KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation.  Blood  1989  73:2003-13  PubMed ID: 2540861  
1774  Fischkoff SA, Kishi K, Benjamin WR, Rossi RM, Hoessly MC, Hoxie JA, Seals C, Anderson L, Ishizaka T, Hakimi J.  Induction of differentiation of the human leukemia cell line, KU812.  Leuk Res  1987  11(12):1105-13  PubMed ID: 3695564   DOI: 10.1016/0145-2126(87)90164-0
1765  Fukuda T, Kishi K, Ohnishi Y, Shibata A.  Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.  Blood   1987  70(3):612-9  PubMed ID: 3304457  
1785  Kishi K.  A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors.  Leuk Res  1985  9(3):381-90  PubMed ID: 3858609   DOI: 10.1016/0145-2126(85)90060-8
1784  Kishi K, Takahashi M, Aoki S, Nagai K, Hirosawa H, Koike T, Sakai C, Aoyagi Y, Sanada M, Moriyama Y, et al.  A new Ph1 positive cell line (KU812) from a patient with blastic crisis of chronic myelogenous leukemia  Nihon Ketsueki Gakkai Zasshi  1984  47(3):709-18  PubMed ID: 6591673  

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User's Publication
12497  Watanabe D, Nogami A, Okada K, Akiyama H, Umezawa Y, Miura O.  FLT3-ITD Activates RSK1 to Enhance Proliferation and Survival of AML Cells by Activating mTORC1 and eIF4B Cooperatively with PIM or PI3K and by Inhibiting Bad and BIM  Cancers (Basel)  2019  11(12):1827  PubMed ID: 31756944   DOI: 10.3390/cancers11121827
7368  Liao EC, Chang CY, Hsieh CW, Yu SJ, Yin SC, Tsai JJ.  An Exploratory Pilot Study of Genetic Marker for IgE-Mediated Allergic Diseases with Expressions of FcεR1α and Cε.  Int J Mol Sci  2015  16:9504-19  PubMed ID: 25923080   DOI: 10.3390/ijms16059504
15072  Tang Y, Abe N, Yoshimoto M, Zhu B, Murata Y, Nakamura Y.  Benzyl isothiocyanate inhibits IL-13 expression in human basophilic KU812 cells  Biosci Biotechnol Biochem  2015  79(1):159-63  PubMed ID: 25253661   DOI: 10.1080/09168451.2014.963503
7936  Motojima H, O Villareal M, Han J, Isoda H.  Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid.  Cytotechnology  2011  63:181-90  PubMed ID: 21331654   DOI: 10.1007/s10616-010-9333-6
8071  Yamada P, Nemoto M, Shigemori H, Yokota S, Isoda H.  Isolation of 5-(hydroxymethyl)furfural from Lycium chinense and its inhibitory effect on the chemical mediator release by basophilic cells.  Planta Med.  2011  77:434-40  PubMed ID: 21031336   DOI: 10.1055/s-0030-1250402
8314  Yamada P, Iijima R, Han J, Shigemori H, Yokota S, Isoda H.  Inhibitory effect of acteoside isolated from Cistanche tubulosa on chemical mediator release and inflammatory cytokine production by RBL-2H3 and KU812 cells.  Planta Med.  2010  76:1512-8  PubMed ID: 20354949   DOI: 10.1055/s-0030-1249775
8529  Wu H, Qi H, Iwasaki D, Zhu B, Shimoishi Y, Murata Y, Nakamura Y.  JNK-dependent NFATc1 pathway positively regulates IL-13 gene expression induced by (-)-epigallocatechin-3-gallate in human basophilic KU812 cells.  Free Radic. Biol. Med.  2009  47:1028-38  PubMed ID: 19607912   DOI: 10.1016/j.freeradbiomed.2009.07.011
12784  Yamada P, Zarrouk M, Kawasaki K, Isoda H.  Inhibitory effect of various Tunisian olive oils on chemical mediator release and cytokine production by basophilic cells  J Ethnopharmacol  2008  116(2):279-87  PubMed ID: 18178046   DOI: 10.1016/j.jep.2007.11.028
16902  Fujita K, Tsujimura A, Miyagawa Y, Kiuchi H, Matsuoka Y, Takao T, Takada S, Nonomura N, Okuyama A.  Isolation of germ cells from leukemia and lymphoma cells in a human in vitro model: potential clinical application for restoring human fertility after anticancer therapy  Cancer Res  2006  66(23):11166-71  PubMed ID: 17145860   DOI: 10.1158/0008-5472.CAN-06-2326
3164  Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto  The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.  Biochemistry  2005  44:6343-9  PubMed ID: 15835923   DOI: 10.1021/bi047606a
2848  Feng, Xingmin, Chuhjo, Tatsuya, Sugimori, Chiharu, Kotani, Takeharu, Lu, Xuzhang, Takami, Akiyoshi, Takamatsu, Hiroyuki, Yamazaki, Hirohito, Nakao, Shinji  Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells.  Blood  2004  104:2425-31  PubMed ID: 15217832   DOI: 10.1182/blood-2004-05-1839
2665  Iwasaki A, Doi T, Umetani M, Watanabe M, Suda M, Hattori Y, Nagoya T  Affinity improvement of the high-affinity immunoglobulin E receptor by phage display.  Biochem Biophys Res Commun  2002  293:542-8  PubMed ID: 12054635   DOI: 10.1016/S0006-291X(02)00261-9
5969  Morita M, Yoshiuchi N, Arakawa H, Nishimura S.  CMAP: a novel cystatin-like gene involved in liver metastasis.  Cancer Res  1999  59(1):151-8  PubMed ID: 9892200  
2240  Matsuo,Y., Okochi, A., Iimura, E., Ohno, T.  Variable Numbers OF Tandem Repeat (VNTR) Amplification by Polymerase ChainReaction For Identification Of Cell Lines  3rd Internet World Congress on Biomedical Sciences in RIKEN 1996  1997     
17237  Isoda H, Kitamoto D, Shinmoto H, Matsumura M, Nakahara T.  Microbial extracellular glycolipid induction of differentiation and inhibition of the protein kinase C activity of human promyelocytic leukemia cell line HL60  Biosci Biotechnol Biochem  1997  61(4):609-14  PubMed ID: 9145519   DOI: 10.1271/bbb.61.609
2119  Yoshinobu Matsuo, Ayumi Okochi, Toshio Ariyasu, Emi Iimura and Tadao Ohno  Identification of Cell Lines with Variabel Numbers of Tandem Repeat(VNTR) Amplified by Polymerase Chain Reaction  Tiss Cult Res Commun  1996  15:211-219    DOI: 10.11418/jtca1981.15.4_21

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