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Cell No. : Cell Name
RCB0494 : NEC15  update : 2022/03/16
CommentEmbryonal carcinoma, testis. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Motoyama, Teiichi
Originator Motoyama, Teiichi
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 20 years
Tissue testis
Disease name teratocacinoma
Classification cancer
History Motoyama - JCRB(FDSC0007)
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3929
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 43-113(50) : /51(3),52(7),53(8),55(3),56(3),57(3)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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1728  Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M  Human testicular germ cell tumors in vitro and in athymic nude mice.  Acta Pathol Jpn  1987  37:431-48  PubMed ID: 3618218   DOI: 10.1111/j.1440-1827.1987.tb00377.x

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User's Publication
6388  Ogino H, Nakayama R, Sakamoto H, Yoshida T, Sugimura T, Masutani M.  Analysis of poly(ADP-ribose) polymerase-1 (PARP1) gene alteration in human germ cell tumor cell lines.  Cancer Genet Cytogenet  2010  197(1):8-15  PubMed ID: 20113831   DOI: 10.1016/j.cancergencyto.2009.10.012

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