Cell No. : Cell Name
RCB0494 : NEC15
update : 2023/05/11
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| Comment | Embryonal carcinoma, testis. Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Motoyama, Teiichi
|
| Originator |
Motoyama, Teiichi
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
20 years
|
| Tissue |
testis
|
| Disease name |
teratocacinoma
|
| Classification |
cancer
|
| History |
Motoyama - JCRB(FDSC0007)
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_3929
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/2 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
43-113(50) : /51(3),52(7),53(8),55(3),56(3),57(3)/
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
1
|
| Reference |
|
249
Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M.
Production of beta-human chorionic gonadotropin by germ cell tumors in vivo and in vitro
Acta Pathol Jpn
1988
38(5):577-90
PubMed ID: 2850705
DOI: 10.1111/j.1440-1827.1988.tb02331.x
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1728
Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M
Human testicular germ cell tumors in vitro and in athymic nude mice.
Acta Pathol Jpn
1987
37:431-48
PubMed ID: 3618218
DOI: 10.1111/j.1440-1827.1987.tb00377.x
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255
Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M.
Production of alpha-fetoprotein by human germ cell tumors in vivo and in vitro
Acta Pathol Jpn
1987
37(8):1263-77
PubMed ID: 2445169
DOI: 10.1111/j.1440-1827.1987.tb00459.x
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254
Sekiya S, Kawata M, Iwasawa H, Inaba N, Sugita M, Suzuki N, Motoyama T, Yamamoto T, Takamizawa H.
Characterization of human embryonal carcinoma cell lines derived from testicular germ-cell tumors
Differentiation
1985
29(3):259-67
PubMed ID: 2416623
DOI: 10.1111/j.1432-0436.1985.tb00325.x
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