Back Back
Cell No. : Cell Name
RCB0490 : NEC14  update : 2022/03/16
CommentEmbryonal carcinoma, testis. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Motoyama, Teiichi
Originator Motoyama, Teiichi
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 26 years
Tissue testis
Disease name teratocarcinoma
Classification cancer
History Motoyama - JCRB(FDSC0006)
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3070
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD, AST, MPI, PepB
Chromosome mode 54-61(50) : 56(4),57(5),58(9),59(8),60(14),61(5)
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 16

To topTop
1728  Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M  Human testicular germ cell tumors in vitro and in athymic nude mice.  Acta Pathol Jpn  1987  37:431-48  PubMed ID: 3618218   DOI: 10.1111/j.1440-1827.1987.tb00377.x

To topTop
User's Publication
11326  Abe T, Kohashi K, Takemoto J, Kinoshita F, Eto M, Oda Y.  Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor.  J Cancer  2018    PubMed ID: 30519350   DOI: 10.7150/jca.25279
6403  Terasaka N, Futai K, Katoh T, Suga H.  A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX.  RNA  2016  22(12):1918-1928  PubMed ID: 27852928   DOI: 10.1261/rna.057737.116
7130  Kita H, Okamoto K, Kushima R, Kawauchi A, Chano T.  Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas.  Oncol Lett  2015  10:661-666  PubMed ID: 26622550   DOI: 10.3892/ol.2015.3306
6395  Ando S, Matsuoka T, Kawai K, Sugita S, Joraku A, Kojima T, Suetomi T, Miyazaki J, Fujita J, Nishiyama H.  Expression of the oncoprotein gankyrin and phosphorylated retinoblastoma protein in human testis and testicular germ cell tumor.  Int J Urol  2014  21(10):992-8  PubMed ID: 24861729   DOI: 10.1111/iju.12484
6398  Nagano K, Yamashita T, Inoue M, Higashisaka K, Yoshioka Y, Abe Y, Mukai Y, Kamada H, Tsutsumi Y, Tsunoda S  Eph receptor A10 has a potential as a target for a prostate cancer therapy.  Biochem Biophys Res Commun  2014  450(1):545-9  PubMed ID: 24924629   DOI: 10.1016/j.bbrc.2014.06.007
6394  Behjati R, Kawai K, Inadome Y, Kano J, Akaza H, Noguchi M.  APAF-1 is related to an undifferentiated state in the testicular germ cell tumor pathway.  Cancer Sci  2011  102(1):267-74  PubMed ID: 20977544   DOI: 10.1111/j.1349-7006.2010.01750.x
8246  Tominaga A, Sugawara H, Futagawa T, Inoue K, Sasaki K, Minamino N, Hatakeyama M, Handa H, Miyata A.  Characterization of the testis-specific promoter region in the human pituitary adenylate cyclase-activating polypeptide (PACAP) gene.  Genes Cells  2010  15:595-606  PubMed ID: 20500521   DOI: 10.1111/j.1365-2443.2010.01403.x
6387  Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, Okamoto K.  DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma.  Clin Cancer Res  2010  16(10):2751-9  PubMed ID: 20460473   DOI: 10.1158/1078-0432.CCR-09-3338
3374  Yanagisawa, Ayano, Endo, Chisato, Okawa, Katsuya, Shitara, Shingo, Kugoh, Hiroyuki, Kakitani, Makoto, Oshimura, Mitsuo, Tomizuka, Kazuma  Generation of chromosome-specific monoclonal antibodies using in vitro-differentiated transchromosomic mouse embryonic stem cells.  Stem Cells  2005  23:1479-88  PubMed ID: 16081665   DOI: 10.1634/stemcells.2004-0369
2888  Sekiguchi T, Iida H, Fukumura J, Nishimoto T  Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation.  Exp Cell Res  2004  300:213-22  PubMed ID: 15383328   DOI: 10.1016/j.yexcr.2004.07.005
6397  Shirakawa T, Gotoh A, Zhang Z, Kao C, Chung LW, Gardner TA.  Development of human chorionic gonadotropin subunit-beta promoter-based toxic gene therapy for testicular cancer.  Urology  2004  63(3):613-8  PubMed ID: 15028478   DOI: 10.1016/j.urology.2003.09.054
6401  Tsuchiya Y, Nakajima M, Itoh S, Iwanari M, Yokoi T.  Expression of aryl hydrocarbon receptor repressor in normal human tissues and inducibility by polycyclic aromatic hydrocarbons in human tumor-derived cell lines.  Toxicol Sci  2003  72(2):253-9  PubMed ID: 12655030   DOI: 10.1093/toxsci/kfg022
6402  Nakajima M, Iwanari M, Yokoi T.  Effects of histone deacetylation and DNA methylation on the constitutive and TCDD-inducible expressions of the human CYP1 family in MCF-7 and HeLa cells.  Toxicol Lett  2003  144(2):247-56  PubMed ID: 12927368   DOI: 10.1016/s0378-4274(03)00216-9
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014   DOI: 10.1111/j.1349-7006.2002.tb02174.x
6399  Iwanari M, Nakajima M, Kizu R, Hayakawa K, Yokoi T.  Induction of CYP1A1, CYP1A2, and CYP1B1 mRNAs by nitropolycyclic aromatic hydrocarbons in various human tissue-derived cells: chemical-, cytochrome P450 isoform-, and cell-specific differences.  Arch Toxicol  2002  76(5-6):287-98  PubMed ID: 12107646   DOI: 10.1007/s00204-002-0340-z
1902  Shu Qin Liu, Kaoru Saijo, Makoto Kodama, Tadao Ohno  Intelligent matrices for tumor-specific cytotoxic T lymphocytes  Materials Science and Engineering: C  1994  2:61-65    DOI: 10.1016/0928-4931(94)90030-2

Back Back Return Top Page