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Cell No. : Cell Name
RCB0489 : NEC8  update : 2023/06/27
CommentEmbryonal carcinoma, testis.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Motoyama, Teiichi
Originator Yamamoto,Takahiko
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 24 years
Tissue testis
Disease name teratocarcinoma
Classification cancer
History Motoyama - JCRB(FDSC0005)
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1604
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, MPI
Chromosome mode 59-132(50) : /60(2),62(7),63(2),64(6),65(8),66(11)/
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 20

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249  Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M.  Production of beta-human chorionic gonadotropin by germ cell tumors in vivo and in vitro  Acta Pathol Jpn  1988  38(5):577-90  PubMed ID: 2850705   DOI: 10.1111/j.1440-1827.1988.tb02331.x
1728  Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M  Human testicular germ cell tumors in vitro and in athymic nude mice.  Acta Pathol Jpn  1987  37:431-48  PubMed ID: 3618218   DOI: 10.1111/j.1440-1827.1987.tb00377.x
255  Motoyama T, Watanabe H, Yamamoto T, Sekiguchi M.  Production of alpha-fetoprotein by human germ cell tumors in vivo and in vitro  Acta Pathol Jpn  1987  37(8):1263-77  PubMed ID: 2445169   DOI: 10.1111/j.1440-1827.1987.tb00459.x
254  Sekiya S, Kawata M, Iwasawa H, Inaba N, Sugita M, Suzuki N, Motoyama T, Yamamoto T, Takamizawa H.  Characterization of human embryonal carcinoma cell lines derived from testicular germ-cell tumors  Differentiation  1985  29(3):259-67  PubMed ID: 2416623   DOI: 10.1111/j.1432-0436.1985.tb00325.x
248  Yamamoto T, Komatsubara S, Suzuki T, Oboshi S.   In vitro cultivation of human testicular embryonal carcinoma and establishment of a new cell line  Gan  1979  70(5):677-80  PubMed ID: 520758  

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User's Publication
21188  Shimada Y, Kato T, Sakurai Y, Watanabe H, Nonaka M, Nanaura N, Ichinoe M, Murakumo Y.  Identification of the promoter region regulating the transcription of the REV7 gene.  Biochem Biophys Res Commun  2023  662:8-17  PubMed ID: 37094431   DOI: 10.1016/j.bbrc.2023.04.056
20916  Kitayama S, Ikeda K, Sato W, Takeshita H, Kawakami S, Inoue S, Horie K.  Testis-expressed gene 11 inhibits cisplatin-induced DNA damage and contributes to chemoresistance in testicular germ cell tumor.  Sci Rep  2022  12(1):18423  PubMed ID: 36319719   DOI: 10.1038/s41598-022-21856-3
13072  Sakurai Y, Ichinoe M, Yoshida K, Nakazato Y, Saito S, Satoh M, Nakada N, Sanoyama I, Umezawa A, Numata Y, Shi-Xu J, Ichihara M, Takahashi M, Murakumo Y.  Inactivation of REV7 enhances chemosensitivity and overcomes acquired chemoresistance in testicular germ cell tumors.  Cancer Lett  2020    PubMed ID: 32553781   DOI: 10.1016/j.canlet.2020.06.001
11326  Abe T, Kohashi K, Takemoto J, Kinoshita F, Eto M, Oda Y.  Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor.  J Cancer  2018    PubMed ID: 30519350   DOI: 10.7150/jca.25279
7130  Kita H, Okamoto K, Kushima R, Kawauchi A, Chano T.  Dimethyl sulfoxide induces chemotherapeutic resistance in the treatment of testicular embryonal carcinomas.  Oncol Lett  2015  10:661-666  PubMed ID: 26622550   DOI: 10.3892/ol.2015.3306
6384  Hidenori Kawashima Sakae Masaki Marie Kawamura  Induction of HLA‑A* 33‑restricted cytotoxic lymphocytes against renal cell carcinoma targeting galectin 9 and PINCH  Biomedical Reports  2014  2:809-812  PubMed ID: 25279150   DOI: 10.3892/br.2014.334
6390  Kawashima H, Obayashi A, Kawamura M, Masaki S, Tamada S, Iguchi T, Uchida J, Kuratsukuri K, Tanaka T, Nakatani T.  Galectin 9 and PINCH, novel immunotherapy targets of renal cell carcinoma: a rationale to find potential tumour antigens and the resulting cytotoxic T lymphocytes induced by the derived peptides.  BJU Int  2014  113(2):320-32  PubMed ID: 24895689   DOI: 10.1111/bju.12499
6395  Ando S, Matsuoka T, Kawai K, Sugita S, Joraku A, Kojima T, Suetomi T, Miyazaki J, Fujita J, Nishiyama H.  Expression of the oncoprotein gankyrin and phosphorylated retinoblastoma protein in human testis and testicular germ cell tumor.  Int J Urol  2014  21(10):992-8  PubMed ID: 24861729   DOI: 10.1111/iju.12484
6398  Nagano K, Yamashita T, Inoue M, Higashisaka K, Yoshioka Y, Abe Y, Mukai Y, Kamada H, Tsutsumi Y, Tsunoda S  Eph receptor A10 has a potential as a target for a prostate cancer therapy.  Biochem Biophys Res Commun  2014  450(1):545-9  PubMed ID: 24924629   DOI: 10.1016/j.bbrc.2014.06.007
15922  Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.  Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers  Proc Natl Acad Sci U S A   2013  110(6):2105-10  PubMed ID: 23345451   DOI: 10.1073/pnas.1214233110
6385  Ushida H, Chano T, Minami K, Kita H, Kawakami T, Okabe H, Okada Y, Okamoto K.  Therapeutic potential of SOX2 inhibition for embryonal carcinoma.  J Urol  2012  187(5):1876-81  PubMed ID: 22425046   DOI: 10.1016/j.juro.2011.12.058
6391  Fujitani N, Takegawa Y, Ishibashi Y, Araki K, Furukawa J, Mitsutake S, Igarashi Y, Ito M, Shinohara Y.  Qualitative and quantitative cellular glycomics of glycosphingolipids based on rhodococcal endoglycosylceramidase-assisted glycan cleavage, glycoblotting-assisted sample preparation, and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry analysis.  J Biol Chem  2011  286(48):41669-79  PubMed ID: 21965662   DOI: 10.1074/jbc.M111.301796
6394  Behjati R, Kawai K, Inadome Y, Kano J, Akaza H, Noguchi M.  APAF-1 is related to an undifferentiated state in the testicular germ cell tumor pathway.  Cancer Sci  2011  102(1):267-74  PubMed ID: 20977544   DOI: 10.1111/j.1349-7006.2010.01750.x
6387  Minami K, Chano T, Kawakami T, Ushida H, Kushima R, Okabe H, Okada Y, Okamoto K.  DNMT3L is a novel marker and is essential for the growth of human embryonal carcinoma.  Clin Cancer Res  2010  16(10):2751-9  PubMed ID: 20460473   DOI: 10.1158/1078-0432.CCR-09-3338
6388  Ogino H, Nakayama R, Sakamoto H, Yoshida T, Sugimura T, Masutani M.  Analysis of poly(ADP-ribose) polymerase-1 (PARP1) gene alteration in human germ cell tumor cell lines.  Cancer Genet Cytogenet  2010  197(1):8-15  PubMed ID: 20113831   DOI: 10.1016/j.cancergencyto.2009.10.012
3374  Yanagisawa, Ayano, Endo, Chisato, Okawa, Katsuya, Shitara, Shingo, Kugoh, Hiroyuki, Kakitani, Makoto, Oshimura, Mitsuo, Tomizuka, Kazuma  Generation of chromosome-specific monoclonal antibodies using in vitro-differentiated transchromosomic mouse embryonic stem cells.  Stem Cells  2005  23:1479-88  PubMed ID: 16081665   DOI: 10.1634/stemcells.2004-0369
2888  Sekiguchi T, Iida H, Fukumura J, Nishimoto T  Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation.  Exp Cell Res  2004  300:213-22  PubMed ID: 15383328   DOI: 10.1016/j.yexcr.2004.07.005
6397  Shirakawa T, Gotoh A, Zhang Z, Kao C, Chung LW, Gardner TA.  Development of human chorionic gonadotropin subunit-beta promoter-based toxic gene therapy for testicular cancer.  Urology  2004  63(3):613-8  PubMed ID: 15028478   DOI: 10.1016/j.urology.2003.09.054
6389  Iwashita S, Koyama K, Nakamura Y.  VNTR sequence on human chromosome 11p15 that affects transcriptional activity.  J Hum Genet  2001  46(12):717-21  PubMed ID: 11776385   DOI: 10.1007/s100380170006
6392  Nadano D, Aoki C, Yoshinaka T, Irie S, Sato TA.  Electrophoretic characterization of ribosomal subunits and proteins in apoptosis: specific downregulation of S11 in staurosporine-treated human breast carcinoma cells.  Biochemistry.   2001  40(50):15184-93  PubMed ID: 11735401   DOI: 10.1021/bi0108397

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