細胞番号 : 細胞名
RCB0488 : 3Y1-B clone 1-6
update : 2022/03/16
|
| 細胞特性(Comment:英) | Standard clone of 3Y1 series. So called 3Y1 |
| 細胞特性(日) | 3Y1シリーズの標準株。従来RCB287を標準株としていたが，樹立者の要請によりこれに置換することとした。性質は変わらない。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | No restriction, but required to cite ref. (Int J Cancer 1975;15:694-706) and to send a reprint to originator. |
| 使用条件(日) | 特に制限を設けない。ただし、文献 (Int J Cancer 1975;15:694-706) を引用し、樹立者に別刷りを送付すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Kimura, Genki
|
| 樹立者 |
Kimura, Genki
|
| 寄託日 |
1990
|
| 別名 |
3Y1
|
| 元の細胞 |
3Y1-B clone 1
|
| 動物種 |
_rat
< Mammals
|
| 動物系統名 |
Fischer
|
| 性別 |
Female
|
| 齢 |
embryo
|
| 採取組織 |
embryo/fetus, whole
|
| 転移 |
No
|
| 細胞分類 |
semi-normal
|
| 初代培養日 |
1970
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_E147
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Adherent cells
|
| 培地 |
|
DMEM (low glucose) + 10% FBS
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
0.25% Trypsin
|
|
継代密度 |
|
1 : 8 split
|
| 継代・培地交換頻度 |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ |
|
(-)
|
| 動物種PCR |
|
OK
|
| アイソザイム検査 |
|
LD, NP, G6PD, MPI, PepB
|
| 染色体検査 |
|
40-45(50) : 40(3),41(4),42(10),43(23),44(9),45(1)
|
| 文献情報 |
Reference(英) |
5件
|
| Reference(日) |
4件
|
| 利用者成果(英) |
10件
|
| 利用者成果(日) |
0件
|
| Reference(英) |
|
1049
Matsuzaki A, Ohno K, Ohtsu M, Kimura G
Functional rescue of temperature-sensitive defects in the cell-cycle mutants of rat 3Y1 fibroblasts by the small t-antigen deletion mutant of simian virus 40.
Cell Struct Funct
1989
14:375-82
PubMed ID: 2553277
DOI: 10.1247/csf.14.375
|
|
1062
Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.
Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro
Biomedical Research
1988
9:181-197
DOI: 10.2220/biomedres.9.181
|
|
2046
Ohno K, Kimura G
Genetic analysis of control of proliferation in fibroblastic cells in culture. II. Alteration in proliferative and survival phenotypes in a set of temperature-sensitive mutants of rat 3Y1 cells after infection or transformation with simian virus 40.
Somat Cell Mol Genet
1984
10:29-36
PubMed ID: 6322358
DOI: 10.1007/BF01534470
|
|
483
Kimura G
Temperature-sensitive growth of cells transformed by ts-a mutant of polyoma virus.
Nature
1975
253:639-41
PubMed ID: 163442
DOI: 10.1038/253639b0
|
|
1048
Kimura G, Itagaki A, Summers J
Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives.
Int J Cancer
1975
15:694-706
PubMed ID: 166944
DOI: 10.1002/ijc.2910150419
|
| 利用者成果(英) |
|
7456
Okina Y, Takeuchi F, Yokomichi T, Takada Y, Kataoka T.
Cardenolide aglycones inhibit tumor necrosis factor α-induced expression of intercellular adhesion molecule-1 at the translation step by blocking Na⁺/K⁺-ATPase
Biol. Pharm. Bull.
2015
38:39-47
PubMed ID: 25744456
DOI: 10.1248/bpb.b14-00532
|
|
6446
Tsuji AB, Sugyo A, Sudo H, Sagara M, Ishikawa A, Ohtsuki M, Kimura T, Ogiu T, Miyagishi M, Taira K, Imai T, Harada YN.
Defective repair of radiation-induced DNA damage is complemented by a CHORI-230-65K18 BAC clone on rat chromosome 4.
Genomics
2006
87(2):236-42
PubMed ID: 16309880
DOI: 10.1016/j.ygeno.2005.09.020
|
|
2649
Itano, Naoki, Atsumi, Fukiko, Sawai, Takahiro, Yamada, Yoichi, Miyaishi, Osamu, Senga, Takeshi, Hamaguchi, Michinari, Kimata, Koji
Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration.
Proc Natl Acad Sci U S A
2002
99:3609-14
PubMed ID: 11891291
DOI: 10.1073/pnas.052026799
|
|
3785
Aoto H, Sasaki H, Ishino M, Sasaki T.
Nuclear translocation of cell adhesion kinase beta/proline-rich tyrosine kinase 2.
Cell Struct Funct
2002
27(1):47-61
PubMed ID: 11937718
DOI: 10.1247/csf.27.47
|
|
2364
Nomura R, Fujimoto T
Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization.
Mol Biol Cell
1999
10:975-86
PubMed ID: 10198051
DOI: 10.1091/mbc.10.4.975
|
|
1942
Takano M, Yokoyama K, Yayama K, Okamoto H
Rat fibroblasts synthesize T-kininogen in response to cyclic-AMP, prostaglandin E2 and cytokines.
Biochim Biophys Acta
1995
1268:107-14
PubMed ID: 7626656
DOI: 10.1016/0167-4889(95)00048-w
|
|
17963
Sasaki H, Nagura K, Ishino M, Tobioka H, Kotani K, Sasaki T.
Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily
J Biol Chem
1995
270(36):21206-19
PubMed ID: 7673154
DOI: 10.1074/jbc.270.36.21206
|
|
17969
Okamoto T, Isoda H, Kubota N, Takahata K, Takahashi T, Kishi T, Nakamura TY, Muromachi Y, Matsui Y, Goshima K.
Melittin cardiotoxicity in cultured mouse cardiac myocytes and its correlation with calcium overload
Toxicol Appl Pharmacol
1995
133(1):150-63
PubMed ID: 7597704
DOI: 10.1006/taap.1995.1136
|
|
17974
Nakamura TY, Yamamoto I, Nishitani H, Matozaki T, Suzuki T, Wakabayashi S, Shigekawa M, Goshima K.
Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration
J Cell Biol
1995
131(2):509-24
PubMed ID: 7593175
DOI: 10.1083/jcb.131.2.509
|
|
1623
Matsuda M, Tanaka S, Nagata S, Kojima A, Kurata T, Shibuya M
Two species of human CRK cDNA encode proteins with distinct biological activities.
Mol Cell Biol
1992
12:3482-9
PubMed ID: 1630456
DOI: 10.1128/mcb.12.8.3482-3489.1992
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト