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Cell No. : Cell Name
RCB0488 : 3Y1-B clone 1-6  update : 2024/05/21
CommentStandard clone of 3Y1 series. So called 3Y1
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Int J Cancer 1975;15:694-706) and to send a reprint to originator.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1990
Another name 3Y1
Original cell 3Y1-B clone 1
Animal _rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Metastatic ability No
Classification semi-normal
Year of origin 1970
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E147
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, MPI, PepB
Chromosome mode 40-45(50) : 40(3),41(4),42(10),43(23),44(9),45(1)
Reference information Reference 5
User's Publication 10

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1049  Matsuzaki A, Ohno K, Ohtsu M, Kimura G  Functional rescue of temperature-sensitive defects in the cell-cycle mutants of rat 3Y1 fibroblasts by the small t-antigen deletion mutant of simian virus 40.  Cell Struct Funct  1989  14:375-82  PubMed ID: 2553277   DOI: 10.1247/csf.14.375
1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181
2046  Ohno K, Kimura G  Genetic analysis of control of proliferation in fibroblastic cells in culture. II. Alteration in proliferative and survival phenotypes in a set of temperature-sensitive mutants of rat 3Y1 cells after infection or transformation with simian virus 40.  Somat Cell Mol Genet  1984  10:29-36  PubMed ID: 6322358   DOI: 10.1007/BF01534470
483  Kimura G  Temperature-sensitive growth of cells transformed by ts-a mutant of polyoma virus.  Nature  1975  253:639-41  PubMed ID: 163442   DOI: 10.1038/253639b0
1048  Kimura G, Itagaki A, Summers J  Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives.  Int J Cancer  1975  15:694-706  PubMed ID: 166944   DOI: 10.1002/ijc.2910150419

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User's Publication
7456  Okina Y, Takeuchi F, Yokomichi T, Takada Y, Kataoka T.  Cardenolide aglycones inhibit tumor necrosis factor α-induced expression of intercellular adhesion molecule-1 at the translation step by blocking Na⁺/K⁺-ATPase  Biol. Pharm. Bull.  2015  38:39-47  PubMed ID: 25744456   DOI: 10.1248/bpb.b14-00532
6446  Tsuji AB, Sugyo A, Sudo H, Sagara M, Ishikawa A, Ohtsuki M, Kimura T, Ogiu T, Miyagishi M, Taira K, Imai T, Harada YN.  Defective repair of radiation-induced DNA damage is complemented by a CHORI-230-65K18 BAC clone on rat chromosome 4.  Genomics  2006  87(2):236-42  PubMed ID: 16309880   DOI: 10.1016/j.ygeno.2005.09.020
2649  Itano, Naoki, Atsumi, Fukiko, Sawai, Takahiro, Yamada, Yoichi, Miyaishi, Osamu, Senga, Takeshi, Hamaguchi, Michinari, Kimata, Koji  Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration.  Proc Natl Acad Sci U S A  2002  99:3609-14  PubMed ID: 11891291   DOI: 10.1073/pnas.052026799
3785  Aoto H, Sasaki H, Ishino M, Sasaki T.  Nuclear translocation of cell adhesion kinase beta/proline-rich tyrosine kinase 2.  Cell Struct Funct  2002  27(1):47-61  PubMed ID: 11937718   DOI: 10.1247/csf.27.47
2364  Nomura R, Fujimoto T  Tyrosine-phosphorylated caveolin-1: immunolocalization and molecular characterization.  Mol Biol Cell  1999  10:975-86  PubMed ID: 10198051   DOI: 10.1091/mbc.10.4.975
1942  Takano M, Yokoyama K, Yayama K, Okamoto H  Rat fibroblasts synthesize T-kininogen in response to cyclic-AMP, prostaglandin E2 and cytokines.  Biochim Biophys Acta  1995  1268:107-14  PubMed ID: 7626656   DOI: 10.1016/0167-4889(95)00048-w
17963  Sasaki H, Nagura K, Ishino M, Tobioka H, Kotani K, Sasaki T.  Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily  J Biol Chem  1995  270(36):21206-19  PubMed ID: 7673154   DOI: 10.1074/jbc.270.36.21206
17969  Okamoto T, Isoda H, Kubota N, Takahata K, Takahashi T, Kishi T, Nakamura TY, Muromachi Y, Matsui Y, Goshima K.  Melittin cardiotoxicity in cultured mouse cardiac myocytes and its correlation with calcium overload  Toxicol Appl Pharmacol  1995  133(1):150-63  PubMed ID: 7597704   DOI: 10.1006/taap.1995.1136
17974  Nakamura TY, Yamamoto I, Nishitani H, Matozaki T, Suzuki T, Wakabayashi S, Shigekawa M, Goshima K.  Detachment of cultured cells from the substratum induced by the neutrophil-derived oxidant NH2Cl: synergistic role of phosphotyrosine and intracellular Ca2+ concentration  J Cell Biol  1995  131(2):509-24  PubMed ID: 7593175   DOI: 10.1083/jcb.131.2.509
1623  Matsuda M, Tanaka S, Nagata S, Kojima A, Kurata T, Shibuya M  Two species of human CRK cDNA encode proteins with distinct biological activities.  Mol Cell Biol  1992  12:3482-9  PubMed ID: 1630456   DOI: 10.1128/mcb.12.8.3482-3489.1992

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