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Cell No. : Cell Name
RCB0486 : CHP-126  update : 2018/07/31
CommentNeuroblastoma. N-myc gene amplification. Cell growth is slow. Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nishi, Yoshisuke
Originator Schlesinger, H. R.
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 14 months
Tissue neuroblastoma
Case history neuroblastoma (stage III)
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 36-92(50) : /42(2),44(4),45(8),46(29),47(3),50(1)/
Reference information Reference 4
User's Publication 1


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Reference
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196
1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592
4689  Balaban-Malenbaum G, Gilbert F.  Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line.  Science  1977  198(4318):739-41  PubMed ID: 71759
1215  Schlesinger, H R, Gerson, J M, Moorhead, P S, Maguire H, Hummeler K  Establishment and characterization of human neuroblastoma cell lines.  Cancer Res  1976  36:3094-100  PubMed ID: 10079

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User's Publication
2916  Abe, Masanobu, Ohira, Miki, Kaneda, Atsushi, Yagi, Yukiko, Yamamoto, Seiichiro, Kitano, Yoshihiro, Takato, Tsuyoshi, Nakagawara, Akira, Ushijima, Toshikazu  CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas.  Cancer Res  2005  65:828-34  PubMed ID: 15705880



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