Cell No. : Cell Name RCB0486 : CHP-126  update : 2023/06/30
Comment			Human cell line derived from Neuroblastoma. N-myc gene is amplified. Application consideration
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Nishi, Yoshisuke
		Originator	Schlesinger, H. R.
		Year of deposit	1990
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	14 months
		Tissue	neuroblastoma
		Disease name	neuroblastoma (stage III)
		Classification	cancer
		History	Latt, S. A.
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_1123
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent/Suspension cells
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		pipetting or 0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		36-92(50) : /42(2),44(4),45(8),46(29),47(3),50(1)/
		STR(human)		OK
Reference information		Reference	5
		User's Publication	1

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Reference

1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196

246  Schwab M, Alitalo K, Klempnauer KH, Varmus HE, Bishop JM, Gilbert F, Brodeur G, Goldstein M, Trent J.  Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour  Nature  1983  305(5931):245-8  PubMed ID: 6888561   DOI: 10.1038/305245a0

1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592   DOI: 10.1016/0165-4608(82)90105-4

4689  Balaban-Malenbaum G, Gilbert F.  Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line.  Science  1977  198(4318):739-41  PubMed ID: 71759   DOI: 10.1126/science.71759

1215  Schlesinger, H R, Gerson, J M, Moorhead, P S, Maguire H, Hummeler K  Establishment and characterization of human neuroblastoma cell lines.  Cancer Res  1976  36:3094-100  PubMed ID: 10079

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User's Publication

2916  Abe, Masanobu, Ohira, Miki, Kaneda, Atsushi, Yagi, Yukiko, Yamamoto, Seiichiro, Kitano, Yoshihiro, Takato, Tsuyoshi, Nakagawara, Akira, Ushijima, Toshikazu  CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas.  Cancer Res  2005  65:828-34  PubMed ID: 15705880