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Cell No. : Cell Name
RCB0485 : LA-N-5  update : 2023/06/30
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified.
Comment from the depositor
Terms and conditionsNon-profit institutions only.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishi, Yoshisuke
Originator Seeger, Robert C.
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue neuroblastoma
Disease name neuroblastoma
Classification cancer
History S. A. Latt
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_0389
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(2),45(5),46(39),47(2),79(1),92(1)
STR(human) OK
Reference information Reference 2
User's Publication 7


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Reference
4699  Cohen PS, Seeger RC, Triche TJ, Israel MA.  Detection of N-myc gene expression in neuroblastoma tumors by in situ hybridization.  Am J Pathol  1988  131(3):391-7  PubMed ID: 2454580  
4698  Slamon DJ, Boone TC, Seeger RC, Keith DE, Chazin V, Lee HC, Souza LM.  Identification and characterization of the protein encoded by the human N-myc oncogene.  Science  1986  232(4751):768-72  PubMed ID: 3008339   DOI: 10.1126/science.3008339

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User's Publication
21471  Hasan MN, Hyodo T, Biswas M, Rahman ML, Mihara Y, Karnan S, Ota A, Tsuzuki S, Hosokawa Y, Konishi H.  Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene.  PLoS One  2023  18(11):e0294146  PubMed ID: 37943774   DOI: 10.1371/journal.pone.0294146
13510  Takahashi Y, Hayakawa A, Sano R, Fukuda H, Harada M, Kubo R, Okawa T, Kominato Y.  Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19  Sci Rep  2021  11(1):3379  PubMed ID: 33564039   DOI: 10.1038/s41598-021-82970-2
12274  Yoichiro Takahashi, Akira Hayakawa, Rie Sano, Haruki Fukuda, Megumi Harada, Rieko Kubo, Takafumi Okawa, Yoshihiko Kominato  Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19  Research Square  2020  23 Sep    DOI: 10.21203/rs.3.rs-82283/v1
15663  Tanimoto T, Tazawa H, Ieda T, Nouso H, Tani M, Oyama T, Urata Y, Kagawa S, Noda T, Fujiwara T.  Elimination of MYCN-Amplified Neuroblastoma Cells by Telomerase-Targeted Oncolytic Virus via MYCN Suppression  Mol Ther Oncolytics  2020  18:14-2  PubMed ID: 32637577   DOI: 10.1016/j.omto.2020.05.015
10828  Saeki N, Saito A, Sugaya Y, Amemiya M, Ono H, Komatsuzaki R, Yanagihara K, Sasaki H.  Chromatin Immunoprecipitation and DNA Sequencing Identified a LIMS1/ILK Pathway Regulated by LMO1 in Neuroblastoma.  Cancer Genomics Proteomics  2018  15:165-174  PubMed ID: 29695398   DOI: 10.21873/cgp.20074
11204  Saeki N, Saito A, Sugaya Y, Amemiya M, Sasaki H.  Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma.  Cancer Genomics Proteomics  2018    PubMed ID: 30194082   DOI: 10.21873/cgp.20100
1969  Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K  Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.  Cancer Lett  1995  96:117-22  PubMed ID: 7553599   DOI: 10.1016/0304-3835(95)03921-i



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