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Cell No. : Cell Name
RCB0485 : LA-N-5  update : 2019/01/18
CommentNeuroblastoma. N-myc gene amplification.
Comment from the depositor
Terms and conditionsNon-profit institutions only.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nishi, Yoshisuke
Originator Seeger, Robert C.
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Tissue neuroblastoma
Case history neuroblastoma
Classification cancer
History S. A. Latt
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(2),45(5),46(39),47(2),79(1),92(1)
Reference information Reference 2
User's Publication 4


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Reference
4699  Cohen PS, Seeger RC, Triche TJ, Israel MA.  Detection of N-myc gene expression in neuroblastoma tumors by in situ hybridization.  Am J Pathol  1988  131(3):391-7  PubMed ID: 2454580
4698  Slamon DJ, Boone TC, Seeger RC, Keith DE, Chazin V, Lee HC, Souza LM.  Identification and characterization of the protein encoded by the human N-myc oncogene.  Science  1986  232(4751):768-72  PubMed ID: 3008339

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User's Publication
12274  Yoichiro Takahashi, Akira Hayakawa, Rie Sano, Haruki Fukuda, Megumi Harada, Rieko Kubo, Takafumi Okawa, Yoshihiko Kominato  Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19  Research Square  2020  23 Sep 
10828  Saeki N, Saito A, Sugaya Y, Amemiya M, Ono H, Komatsuzaki R, Yanagihara K, Sasaki H.  Chromatin Immunoprecipitation and DNA Sequencing Identified a LIMS1/ILK Pathway Regulated by LMO1 in Neuroblastoma.  Cancer Genomics Proteomics  2018  15:165-174  PubMed ID: 29695398
11204  Saeki N, Saito A, Sugaya Y, Amemiya M, Sasaki H.  Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma.  Cancer Genomics Proteomics  2018    PubMed ID: 30194082
1969  Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K  Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.  Cancer Lett  1995  96:117-22  PubMed ID: 7553599



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