Cell No. : Cell Name RCB0485 : LA-N-5  update : 2022/07/08
Comment			Human cell line derived from Neuroblastoma. N-myc gene is amplified.
Comment from the depositor
Terms and conditions			Non-profit institutions only.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Nishi, Yoshisuke
		Originator	Seeger, Robert C.
		Year of deposit	1990
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	neuroblastoma
		Disease name	neuroblastoma
		Classification	cancer
		History	S. A. Latt
		Memo_1	Mycoplasma was eliminated with MC-210.
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_0389
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Medium and additives		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		pipetting or 0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		44-92(50) : 44(2),45(5),46(39),47(2),79(1),92(1)
		STR(human)		OK
Reference information		Reference	2
		User's Publication	6

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Reference

4699  Cohen PS, Seeger RC, Triche TJ, Israel MA.  Detection of N-myc gene expression in neuroblastoma tumors by in situ hybridization.  Am J Pathol  1988  131(3):391-7  PubMed ID: 2454580

4698  Slamon DJ, Boone TC, Seeger RC, Keith DE, Chazin V, Lee HC, Souza LM.  Identification and characterization of the protein encoded by the human N-myc oncogene.  Science  1986  232(4751):768-72  PubMed ID: 3008339   DOI: 10.1126/science.3008339

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User's Publication

13510  Takahashi Y, Hayakawa A, Sano R, Fukuda H, Harada M, Kubo R, Okawa T, Kominato Y.  Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19  Sci Rep  2021  11(1):3379  PubMed ID: 33564039   DOI: 10.1038/s41598-021-82970-2

12274  Yoichiro Takahashi, Akira Hayakawa, Rie Sano, Haruki Fukuda, Megumi Harada, Rieko Kubo, Takafumi Okawa, Yoshihiko Kominato  Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19  Research Square  2020  23 Sep    DOI: 10.21203/rs.3.rs-82283/v1

15663  Tanimoto T, Tazawa H, Ieda T, Nouso H, Tani M, Oyama T, Urata Y, Kagawa S, Noda T, Fujiwara T.  Elimination of MYCN-Amplified Neuroblastoma Cells by Telomerase-Targeted Oncolytic Virus via MYCN Suppression  Mol Ther Oncolytics  2020  18:14-2  PubMed ID: 32637577   DOI: 10.1016/j.omto.2020.05.015

10828  Saeki N, Saito A, Sugaya Y, Amemiya M, Ono H, Komatsuzaki R, Yanagihara K, Sasaki H.  Chromatin Immunoprecipitation and DNA Sequencing Identified a LIMS1/ILK Pathway Regulated by LMO1 in Neuroblastoma.  Cancer Genomics Proteomics  2018  15:165-174  PubMed ID: 29695398   DOI: 10.21873/cgp.20074

11204  Saeki N, Saito A, Sugaya Y, Amemiya M, Sasaki H.  Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma.  Cancer Genomics Proteomics  2018    PubMed ID: 30194082   DOI: 10.21873/cgp.20100

1969  Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K  Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.  Cancer Lett  1995  96:117-22  PubMed ID: 7553599   DOI: 10.1016/0304-3835(95)03921-i