Cell No. : Cell Name
RCB0485 : LA-N-5
update : 2023/06/30
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| Comment | Human cell line derived from Neuroblastoma. N-myc gene is amplified. |
| Comment from the depositor | |
| Terms and conditions | Non-profit institutions only. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishi, Yoshisuke
|
| Originator |
Seeger, Robert C.
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
neuroblastoma
|
| Disease name |
neuroblastoma
|
| Classification |
cancer
|
| History |
S. A. Latt
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_0389
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
pipetting or 0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Virus (HIV) |
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(-)
|
| Isozyme |
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LD, NP
|
| Chromosome mode |
|
44-92(50) : 44(2),45(5),46(39),47(2),79(1),92(1)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
7
|
| User's Publication |
|
21471
Hasan MN, Hyodo T, Biswas M, Rahman ML, Mihara Y, Karnan S, Ota A, Tsuzuki S, Hosokawa Y, Konishi H.
Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene.
PLoS One
2023
18(11):e0294146
PubMed ID: 37943774
DOI: 10.1371/journal.pone.0294146
|
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13510
Takahashi Y, Hayakawa A, Sano R, Fukuda H, Harada M, Kubo R, Okawa T, Kominato Y.
Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19
Sci Rep
2021
11(1):3379
PubMed ID: 33564039
DOI: 10.1038/s41598-021-82970-2
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12274
Yoichiro Takahashi, Akira Hayakawa, Rie Sano, Haruki Fukuda, Megumi Harada, Rieko Kubo, Takafumi Okawa, Yoshihiko Kominato
Histone deacetylase inhibitors suppress ACE2 and ABO simultaneously, suggesting a preventive potential against COVID-19
Research Square
2020
23 Sep
DOI: 10.21203/rs.3.rs-82283/v1
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15663
Tanimoto T, Tazawa H, Ieda T, Nouso H, Tani M, Oyama T, Urata Y, Kagawa S, Noda T, Fujiwara T.
Elimination of MYCN-Amplified Neuroblastoma Cells by Telomerase-Targeted Oncolytic Virus via MYCN Suppression
Mol Ther Oncolytics
2020
18:14-2
PubMed ID: 32637577
DOI: 10.1016/j.omto.2020.05.015
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10828
Saeki N, Saito A, Sugaya Y, Amemiya M, Ono H, Komatsuzaki R, Yanagihara K, Sasaki H.
Chromatin Immunoprecipitation and DNA Sequencing Identified a LIMS1/ILK Pathway Regulated by LMO1 in Neuroblastoma.
Cancer Genomics Proteomics
2018
15:165-174
PubMed ID: 29695398
DOI: 10.21873/cgp.20074
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11204
Saeki N, Saito A, Sugaya Y, Amemiya M, Sasaki H.
Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma.
Cancer Genomics Proteomics
2018
PubMed ID: 30194082
DOI: 10.21873/cgp.20100
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1969
Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K
Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.
Cancer Lett
1995
96:117-22
PubMed ID: 7553599
DOI: 10.1016/0304-3835(95)03921-i
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