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Cell No. : Cell Name
RCB0484 : LA-N-2  update : 2023/06/30
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified.
Comment from the depositor
Terms and conditionsNon-profit institutions only.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishi, Yoshisuke
Originator Seeger, Robert C.
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 3 years
Tissue nervous system
Disease name neuroblastoma (stage IV)
Metastatic ability Yes
Metastatic tissue abdominal mass
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1829
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD, AST, MPI
Chromosome mode 70-152(50) : /74(2),75(11),76(13),77(4),78(6),79(4)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 6


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Reference
4676  Spengler BA, Ross RA, Biedler JL.  Differential drug sensitivity of human neuroblastoma cells.  Cancer Treat Rep  1986  70(8):959-65  PubMed ID: 3731152  
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196  
1122  Seeger, R C, Rayner, S A, Banerjee A, Chung H, Laug, W E, Neustein, H B, Benedict, W F  Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.  Cancer Res  1977  37:1364-71  PubMed ID: 856461  
4694  West GJ, Uki J, Herschman HR, Seeger RC.  Adrenergic, cholinergic, and inactive human neuroblastoma cell lines with the action-potential Na+ ionophore.  Cancer Res  1977  37(5):1372-6  PubMed ID: 15722  

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User's Publication
8022  Yamada T, Inazu M, Tajima H, Matsumiya T.  Functional expression of choline transporter-like protein 1 (CTL1) in human neuroblastoma cells and its link to acetylcholine synthesis.  Neurochem. Int.  2011  58:354-65  PubMed ID: 21185344   DOI: 10.1016/j.neuint.2010.12.011
5014  Inoue K, Ito Y.  Neuroblastoma cell proliferation is sensitive to changes in levels of RUNX1 and RUNX3 protein.  Gene  2011  487(2):151-5  PubMed ID: 21640801   DOI: 10.1016/j.gene.2011.05.016
8802  Inoue K, Ito K, Osato M, Lee B, Bae SC, Ito Y.  The transcription factor Runx3 represses the neurotrophin receptor TrkB during lineage commitment of dorsal root ganglion neurons.  J. Biol. Chem.  2007  282:24175-84  PubMed ID: 17584746   DOI: 10.1074/jbc.M703746200
2214  Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K  Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.  Cell Struct Funct  1997  22:511-6  PubMed ID: 9431455   DOI: 10.1247/csf.22.511
17984  Nishimura E, Sasaki K, Maruyama K, Tsukada T, Yamaguchi K.  Decrease in neuron-restrictive silencer factor (NRSF) mRNA levels during differentiation of cultured neuroblastoma cells  Neurosci Lett  1996  211(2):101-4  PubMed ID: 8830854   DOI: 10.1016/0304-3940(96)12722-1
1969  Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K  Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.  Cancer Lett  1995  96:117-22  PubMed ID: 7553599   DOI: 10.1016/0304-3835(95)03921-i



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