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Cell No. : Cell Name
RCB0483 : LA-N-1  update : 2023/06/30
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified. Derived from metastatic tumor in bone marrow.
Comment from the depositor
Terms and conditionsNon-profit institutions only.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Yoshisuke
Originator Seeger, Robert C.
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 2 years
Tissue bone marrow
Disease name neuroblastoma (stage IV)
Metastatic ability Yes
Metastatic tissue bone marrow
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1827
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin (1min.) or pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 69-83(50) : /78(3),79(3),80(3),81(13),82(18),83(5)
STR(human) OK
deposit info
lot info
Reference information Reference 8
User's Publication 11

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4701  Chen RL1, Reynolds CP, Seeger RC.  Neutrophils are cytotoxic and growth-inhibiting for neuroblastoma cells with an anti-GD2 antibody but, without cytotoxicity, can be growth-stimulating.  Cancer Immunol Immunother.  2000  48(11):603-12  PubMed ID: 10663607   DOI: 10.1007/s002620050008
247  Uçar K, Seeger RC, Challita PM, Watanabe CT, Yen TL, Morgan JP, Amado R, Chou E, McCallister T, Barber JR, et al.   Sustained cytokine production and immunophenotypic changes in human neuroblastoma cell lines transduced with a human gamma interferon vector  Cancer Gene Ther  1995  2(3):171-81  PubMed ID: 8528960  
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196  
4697  Sidell N, Altman A, Haussler MR, Seeger RC.  Effects of retinoic acid (RA) on the growth and phenotypic expression of several human neuroblastoma cell lines.  Exp Cell Res  1983  148(1):21-30  PubMed ID: 6313408   DOI: 10.1016/0014-4827(83)90184-2
4695  Danon YL, Epstein MB, Siegel MM, Rucker N, Myers CP, Norman A, Benedict WF, Seeger RC.  Variability in DNA distributions of human neuroblastomas after cyclophosphamide.  Cancer Treat Rep  1980  64(12):1275-81  PubMed ID: 7471116  
4696  Siegel MM, Chung HS, Rucker N, Siegel SE, Seeger RC, Isaacs H Jr, Benedict WF.  In vitro and in vivo preclinical chemotherapy studies of human neuroblastoma.  Cancer Treat Rep  1980  64(8-9):975-9  PubMed ID: 7448832  
1122  Seeger, R C, Rayner, S A, Banerjee A, Chung H, Laug, W E, Neustein, H B, Benedict, W F  Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.  Cancer Res  1977  37:1364-71  PubMed ID: 856461  
4694  West GJ, Uki J, Herschman HR, Seeger RC.  Adrenergic, cholinergic, and inactive human neuroblastoma cell lines with the action-potential Na+ ionophore.  Cancer Res  1977  37(5):1372-6  PubMed ID: 15722  

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User's Publication
21143  Ma G, Tan C, Shan Y, Shao N, Wang F, Dimitrov DS, Wang L, Zhao Q.  An insulin growth factor-I/II-neutralizing monoclonal antibody in combination with epidermal growth factor receptor inhibitors potently inhibits tumor cell growth.  J Cancer  2022  13(6):1830-1836  PubMed ID: 35399718   DOI: 10.7150/jca.69064
10728  Kurita M, Takada T, Wakabayashi N, Asami S, Ono S, Uchiyama T, Suzuki T.  Antitumor Effect of Burchellin Derivatives Against Neuroblastoma.  Anticancer Res.  2018  38:855-862  PubMed ID: 29374712   DOI: 10.21873/anticanres.12294
14467  Tabata K, Nishimura Y, Takeda T, Kurita M, Uchiyama T, Suzuki T.  Sesquiterpene lactones derived from Saussurea lappa induce apoptosis and inhibit invasion and migration in neuroblastoma cells  J Pharmacol Sci  2015  127(4):397-403  PubMed ID: 25953266   DOI: 10.1016/j.jphs.2015.01.002
15018  So WW, Liu WN, Leung KN.  Omega-3 Polyunsaturated Fatty Acids Trigger Cell Cycle Arrest and Induce Apoptosis in Human Neuroblastoma LA-N-1 Cells  Nutrients  2015  7(8):6956-73  PubMed ID: 26295255   DOI: 10.3390/nu7085319
18238  Liao X, Leung KN.  Tryptanthrin induces growth inhibition and neuronal differentiation in the human neuroblastoma LA-N-1 cells  Chem Biol Interact  2013  203(2):512-21  PubMed ID: 23500671   DOI: 10.1016/j.cbi.2013.03.001
12725  Kitano T, Yoda H, Tabata K, Miura M, Toriyama M, Motohashi S, Suzuki T.  Vitamin K3 analogs induce selective tumor cytotoxicity in neuroblastoma  Biol Pharm Bull  2012  35(4):617-23  PubMed ID: 22466570   DOI: 10.1248/bpb.35.617
15082  Tabata K, Kim M, Makino M, Satoh M, Satoh Y, Suzuki T.  Phenolic diterpenes derived from Hyptis incana induce apoptosis and G(2)/M arrest of neuroblastoma cells  Anticancer Res  2012  32(11):4781-9  PubMed ID: 23155243  
7941  Hayama T, Tabata K, Uchiyama T, Fujimoto Y, Suzuki T.  Ferrearin C induces apoptosis via heme oxygenase-1 (HO-1) induction in neuroblastoma.  J Nat Med  2011  65:431-9  PubMed ID: 21327520   DOI: 10.1007/s11418-011-0514-1
9486  F H Lo, N K Mak, K N Leung  Studies on the Anti-Tumor Activities of the Soy Isoflavone Daidzein on Murine Neuroblastoma Cells  Biomed Pharmacother  2007  61(9):591-5  PubMed ID: 17905565   DOI: 10.1016/j.biopha.2007.08.021
17984  Nishimura E, Sasaki K, Maruyama K, Tsukada T, Yamaguchi K.  Decrease in neuron-restrictive silencer factor (NRSF) mRNA levels during differentiation of cultured neuroblastoma cells  Neurosci Lett  1996  211(2):101-4  PubMed ID: 8830854   DOI: 10.1016/0304-3940(96)12722-1
1969  Maruyama K, Tsukada T, Bandoh S, Sasaki K, Ohkura N, Yamaguchi K  Expression of NOR-1 and its closely related members of the steroid/thyroid hormone receptor superfamily in human neuroblastoma cell lines.  Cancer Lett  1995  96:117-22  PubMed ID: 7553599   DOI: 10.1016/0304-3835(95)03921-i

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