Cell No. : Cell Name
RCB0482 : MC-NB-1
update : 2023/06/30
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| Comment | Human cell line derived from Neuroblastoma. Possess N-myc gene amplicon. Application consideration |
| Comment from the depositor | |
| Terms and conditions | Non-profit institutions only. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nishi, Yoshisuke
|
| Originator |
James T. Casper
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
2 years
|
| Tissue |
bone marrow
|
| Disease name |
neuroblastoma
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
bone marrow, liver
|
| Classification |
cancer
|
| History |
Latt, S. A.
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_E058
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent/Suspension cells
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| Culture medium |
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RPMI1640 + 15% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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pipetting or 0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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(-)
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| Isozyme |
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LD, NP, G6PD
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| Chromosome mode |
|
42-89(50) : 42(2),43(3),44(8),45(19),46(17),89(1)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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