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Cell No. : Cell Name
RCB0482 : MC-NB-1  update : 2022/04/15
CommentHuman cell line derived from Neuroblastoma. Possess N-myc gene amplicon. Application consideration
Comment from the depositor
Terms and conditionsNon-profit institutions only.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishi, Yoshisuke
Originator James T. Casper
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 2 years
Tissue bone marrow
Disease name neuroblastoma
Metastastatic ability Yes
Metastatsd tissue bone marrow, liver
Classification cancer
History Latt, S. A.
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_E058
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 15% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD
Chromosome mode 42-89(50) : 42(2),43(3),44(8),45(19),46(17),89(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
1124  Casper, J T, Trent, J M, Harb J, Piaskowski V, Helmsworth M, Finlan J, Von Hoff, D D  Distinguishing characteristics of a new neuroblastoma cell line.  Cancer Genet Cytogenet  1983  10:177-86  PubMed ID: 6616438   DOI: 10.1016/0165-4608(83)90122-x

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