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Cell No. : Cell Name
RCB0481 : TNB1  update : 2022/04/15
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified. Another name is TGW (PMID: 2335222). Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Yoshisuke
Originator Tsuchida, Yoshiaki
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 20 months
Tissue neuroblastoma
Disease name neuroblastoma
Metastatic ability Yes
Metastatic tissue mediastinum
Classification cancer
History Latt, S. A. - Tsuchida, Y.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_A443
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 15% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 46-97(50) : 46(4),47(8),48(32),49(2),50(1),73(1)/
STR(human) OK
Reference information Reference 6
User's Publication 6

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4690  Tsuchida Y, Sekiguchi M, Kaneko Y, Kanda N.  Origin of human neuroblastoma cell lines TGW and TNB1.  FEBS Lett  1990  263(2):191  PubMed ID: 2335222   DOI: 10.1016/0014-5793(90)81370-4
4692  Tsuchida Y1, Yokomori K, Kanda N, Kaneko Y, Shimatake H, Hashizume K.  Human neuroblastoma cell line TNB1 and its biological characteristics  Hum Cell  1989  2(2):202-3  PubMed ID: 2577250  
4691  Tsuchida Y1, Kanda N, Shimatake H, Kaneko Y, Notomi T.  Clinical significance of gene amplification studied in human neuroblastoma xenografts: relationship with tumor growth rate, chemotherapeutic sensitivities and levels of neuron-specific enolase.  Exp Cell Biol  1988  56(5):277-84  PubMed ID: 3229554   DOI: 10.1159/000163491
245  Kanda N, Tsuchida Y, Hata J, Kohl NE, Alt FW, Latt SA, Utakoji T.  Amplification of IMR-32 clones 8, G21, and N-myc in human neuroblastoma xenografts  Cancer Res  1987  47(12):3291-5  PubMed ID: 3034408  
244  Kaneko Y, Tsuchida Y, Maseki N, Takasaki N, Sakurai M, Saito S.  Chromosome findings in human neuroblastomas xenografted in nude mice  Jpn J Cancer Res  1985  76(5):359-64  PubMed ID: 3924709  
1125  Tsuchida Y, Yokomori K, Iwanaka T, Saito S  Nude mouse xenograft study for treatment of neuroblastoma: effects of chemotherapeutic agents and surgery on tumor growth and cell kinetics.  J Pediatr Surg  1984  19:72-6  PubMed ID: 6583377   DOI: 10.1016/s0022-3468(84)80020-2

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User's Publication
20778  Chen L, Mu B, Li Y, Lu F, Mu P.  DRR1 promotes neuroblastoma cell differentiation by regulating CREB expression.  Pediatr Res  2022    PubMed ID: 35854089   DOI: 10.1038/s41390-022-02192-8
10685  Su Z, Kishida S, Tsubota S, Sakamoto K, Cao D, Kiyonari S, Ohira M, Kamijo T, Narita A, Xu Y, Takahashi Y, Kadomatsu K.  Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes.  Oncotarget  2017  8:106296-106310  PubMed ID: 29290949   DOI: 10.18632/oncotarget.22435
14685  Mu P, Akashi T, Lu F, Kishida S, Kadomatsu K.  A novel nuclear complex of DRR1, F-actin and COMMD1 involved in NF-κB degradation and cell growth suppression in neuroblastoma  Oncogene  2017  36(41):5745-5756.  PubMed ID: 28604741   DOI: 10.1038/onc.2017.181
15135  Nakaguro M, Kiyonari S, Kishida S, Cao D, Murakami-Tonami Y, Ichikawa H, Takeuchi I, Nakamura S, Kadomatsu K.  Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation  Cancer Sci  2015  106(3):237-43  PubMed ID: 25557119   DOI: 10.1111/cas.12598
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014   DOI: 10.1111/j.1349-7006.2002.tb02174.x
15591  Miyake I, Hakomori Y, Shinohara A, Gamou T, Saito M, Iwamatsu A, Sakai R.  Activation of anaplastic lymphoma kinase is responsible for hyperphosphorylation of ShcC in neuroblastoma cell lines  Oncogene  2002  21(38):5823-34  PubMed ID: 12185581   DOI: 10.1038/sj.onc.1205735

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