細胞番号 : 細胞名
RCB0480 : NB69
update : 2022/04/15
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| 細胞特性(Comment:英) | Human cell line derived from Neuroblastoma. N-myc gene is not amplified. Application consideration |
| 細胞特性(日) | ヒト神経芽細胞腫由来細胞株。N-myc遺伝子の増幅なし。培養開始にあたっての注意 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Nishi, Yoshisuke
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| 樹立者 |
Gilbert, F.
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| 寄託日 |
1990
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| 動物種 |
human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Male
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| 齢 |
16 months
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| 採取組織 |
nervous system
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| 病名 |
neuroblastoma (stage III)
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| 細胞分類 |
cancer
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| 入手歴 |
Latt, S. A.
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|
細胞寿命 |
infinite
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| 細胞形態 |
other
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| Cellosaurus(Expasy) |
CVCL_1448
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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RPMI1640 + 15% FBS
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| 抗生物質 |
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Free
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|
継代方法 |
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pipetting or 0.25% Trypsin (1min.)
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|
継代密度 |
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1 : 4 split
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| 継代・培地交換頻度 |
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Subculture : once/10 days, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
|
5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| ウィルス（HIV) |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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65-94(50) : 82(2),83(1),84(7),85(2),87(1),89(2)/
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
2件
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| Reference(日) |
0件
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| 利用者成果(英) |
14件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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21181
Liu C, Kudo T, Ye X, Gascoigne K.
Cell-to-cell variability in Myc dynamics drives transcriptional heterogeneity in cancer cells.
Cell Rep
2023
42(4):112401
PubMed ID: 37060565
DOI: 10.1016/j.celrep.2023.112401
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11511
Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature
2019
PubMed ID: 30971826
DOI: 10.1038/s41586-019-1103-9
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11593
Hirano T, Nagasaki-Maeoka E, Ishizuka Y, Takatori A, Watanabe Y, Hoshi R, Yoshizawa S, Kawashima H, Uekusa S, Sugito K, Uehara S, Fukuda N, Nagase H, Takayama T, Soma M, Koshinaga T, Fujiwara K.
Forced expression of NR4A3 induced the differentiation of human neuroblastoma-derived NB1 cells.
Med. Oncol.
2019
PubMed ID: 31183633
DOI: 10.1007/s12032-019-1289-6
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12527
Yoda H, Inoue T, Shinozaki Y, Lin J, Watanabe T, Koshikawa N, Takatori A, Nagase H.
Direct Targeting of MYCN Gene Amplification by Site-Specific DNA Alkylation in Neuroblastoma
Cancer Res
2019
79(4):830-840.
PubMed ID: 30584073
DOI: 10.1158/0008-5472.CAN-18-1198
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4309
Ishizuka Y, Koshinaga T, Hirano T, Nagasaki-Maeoka E, Watanabe Y, Hoshi R, Yoshizawa S, Sugito K, Kawashima H, Uekusa S, Fukuda N, Soma M, Fujiwara K.
NRP1 knockdown promotes the migration and invasion of human neuroblastoma-derived SK‑N‑AS cells via the activation of β1 integrin expression.
Int. J. Oncol.
2018
PubMed ID: 29750423
DOI: 10.3892/ijo.2018.4397
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14739
Takagi M, Yoshida M, Nemoto Y, Tamaichi H, Tsuchida R, Seki M, Uryu K, Nishii R, Miyamoto S, Saito M, Hanada R, Kaneko H, Miyano S, Kataoka K, Yoshida K, Ohira M, Hayashi Y, Nakagawara A, Ogawa S, Mizutani S, Takita J.
Loss of DNA Damage Response in Neuroblastoma and Utility of a PARP Inhibitor
J Natl Cancer Inst
2017
109(11)
PubMed ID: 29059438
DOI: 10.1093/jnci/djx062
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7960
Wang J, Oue T, Uehara S, Yamanaka H, Oji Y, Fukuzawa M.
The role of WT1 gene in neuroblastoma.
J. Pediatr. Surg.
2011
46:326-31
PubMed ID: 21292082
DOI: 10.1016/j.jpedsurg.2010.11.011
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9586
Kazuko Yamazaki, Emiko Yamada, Yoshio Kanaji, Tetsuo Yanagisawa, Yoshiyuki Kato, Kanji Sato, Kazue Takano, Yuji Sakasegawa, Kiyotoshi Kaneko
Stimulation of Cellular Prion Protein Expression by TSH in Human Thyrocytes
Biochem Biophys Res Commun
2003
305(4):1034-9
PubMed ID: 12767934
DOI: 10.1016/s0006-291x(03)00801-5
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2758
Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao
Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.
Jpn J Cancer Res
2002
93:313-9
PubMed ID: 11927014
DOI: 10.1111/j.1349-7006.2002.tb02174.x
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16916
Kitamuro T, Takahashi K, Totsune K, Nakayama M, Murakami O, Hida W, Shirato K, Shibahara S.
Differential expression of adrenomedullin and its receptor component, receptor activity modifying protein (RAMP) 2 during hypoxia in cultured human neuroblastoma cells
Peptides
2001
1795-801
PubMed ID: 11754965
DOI: 10.1016/s0196-9781(01)00520-4
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9022
T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma
The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection
J Gen Virol
2000
81 (Pt 7), 1763-71
PubMed ID: 10859382
DOI: 10.1099/0022-1317-81-7-1763
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2358
Kimura M, Matsuda Y, Yoshioka T, Okano Y
Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
J Biol Chem
1999
274:7334-40
PubMed ID: 10066797
DOI: 10.1074/jbc.274.11.7334
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2182
Kimura M, Kotani S, Hattori T, Sumi N, Yoshioka T, Todokoro K, Okano Y
Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.
J Biol Chem
1997
272:13766-71
PubMed ID: 9153231
DOI: 10.1074/jbc.272.21.13766
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2431
Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley, R A, Tsuji A, Matsuda Y
Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.
Infect Immun
1996
64:3093-100
PubMed ID: 8757839
DOI: 10.1128/IAI.64.8.3093-3100.1996
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