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Cell No. : Cell Name
RCB0480 : NB69  update : 2023/06/30
CommentHuman cell line derived from Neuroblastoma. N-myc gene is not amplified. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishi, Yoshisuke
Originator Gilbert, F.
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 16 months
Tissue nervous system
Disease name neuroblastoma (stage III)
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1448
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 15% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin (1min.)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 65-94(50) : 82(2),83(1),84(7),85(2),87(1),89(2)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 15


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Reference
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196  
243  Feder MK, Gilbert F.   Clonal evolution in a human neuroblastoma  J Natl Cancer Inst  1983  70(6):1051-6  PubMed ID: 6574274  
1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592   DOI: 10.1016/0165-4608(82)90105-4

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User's Publication
21181  Liu C, Kudo T, Ye X, Gascoigne K.  Cell-to-cell variability in Myc dynamics drives transcriptional heterogeneity in cancer cells.  Cell Rep  2023  42(4):112401  PubMed ID: 37060565   DOI: 10.1016/j.celrep.2023.112401
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826   DOI: 10.1038/s41586-019-1103-9
11593  Hirano T, Nagasaki-Maeoka E, Ishizuka Y, Takatori A, Watanabe Y, Hoshi R, Yoshizawa S, Kawashima H, Uekusa S, Sugito K, Uehara S, Fukuda N, Nagase H, Takayama T, Soma M, Koshinaga T, Fujiwara K.  Forced expression of NR4A3 induced the differentiation of human neuroblastoma-derived NB1 cells.  Med. Oncol.  2019    PubMed ID: 31183633   DOI: 10.1007/s12032-019-1289-6
12527  Yoda H, Inoue T, Shinozaki Y, Lin J, Watanabe T, Koshikawa N, Takatori A, Nagase H.  Direct Targeting of MYCN Gene Amplification by Site-Specific DNA Alkylation in Neuroblastoma  Cancer Res  2019  79(4):830-840.  PubMed ID: 30584073   DOI: 10.1158/0008-5472.CAN-18-1198
4309  Ishizuka Y, Koshinaga T, Hirano T, Nagasaki-Maeoka E, Watanabe Y, Hoshi R, Yoshizawa S, Sugito K, Kawashima H, Uekusa S, Fukuda N, Soma M, Fujiwara K.  NRP1 knockdown promotes the migration and invasion of human neuroblastoma-derived SK‑N‑AS cells via the activation of β1 integrin expression.  Int. J. Oncol.  2018    PubMed ID: 29750423   DOI: 10.3892/ijo.2018.4397
14739  Takagi M, Yoshida M, Nemoto Y, Tamaichi H, Tsuchida R, Seki M, Uryu K, Nishii R, Miyamoto S, Saito M, Hanada R, Kaneko H, Miyano S, Kataoka K, Yoshida K, Ohira M, Hayashi Y, Nakagawara A, Ogawa S, Mizutani S, Takita J.  Loss of DNA Damage Response in Neuroblastoma and Utility of a PARP Inhibitor  J Natl Cancer Inst  2017  109(11)  PubMed ID: 29059438   DOI: 10.1093/jnci/djx062
7960  Wang J, Oue T, Uehara S, Yamanaka H, Oji Y, Fukuzawa M.  The role of WT1 gene in neuroblastoma.  J. Pediatr. Surg.  2011  46:326-31  PubMed ID: 21292082   DOI: 10.1016/j.jpedsurg.2010.11.011
9586  Kazuko Yamazaki, Emiko Yamada, Yoshio Kanaji, Tetsuo Yanagisawa, Yoshiyuki Kato, Kanji Sato, Kazue Takano, Yuji Sakasegawa, Kiyotoshi Kaneko  Stimulation of Cellular Prion Protein Expression by TSH in Human Thyrocytes  Biochem Biophys Res Commun  2003  305(4):1034-9  PubMed ID: 12767934   DOI: 10.1016/s0006-291x(03)00801-5
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014   DOI: 10.1111/j.1349-7006.2002.tb02174.x
16916  Kitamuro T, Takahashi K, Totsune K, Nakayama M, Murakami O, Hida W, Shirato K, Shibahara S.  Differential expression of adrenomedullin and its receptor component, receptor activity modifying protein (RAMP) 2 during hypoxia in cultured human neuroblastoma cells  Peptides  2001  1795-801  PubMed ID: 11754965   DOI: 10.1016/s0196-9781(01)00520-4
9022  T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma  The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection  J Gen Virol  2000  81 (Pt 7), 1763-71  PubMed ID: 10859382   DOI: 10.1099/0022-1317-81-7-1763
2358  Kimura M, Matsuda Y, Yoshioka T, Okano Y  Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.  J Biol Chem  1999  274:7334-40  PubMed ID: 10066797   DOI: 10.1074/jbc.274.11.7334
2182  Kimura M, Kotani S, Hattori T, Sumi N, Yoshioka T, Todokoro K, Okano Y  Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.  J Biol Chem  1997  272:13766-71  PubMed ID: 9153231   DOI: 10.1074/jbc.272.21.13766
18109  Maeda T, Nishimura N, Nakamura H, Sano K.  p21 (WAF1/Cip1/Sdi1/Pic1) mRNA is expressed in neuroblastoma cell lines but not in Ewing's sarcoma and primitive neuroectodermal tumor cell lines  Acta Paediatr Jpn  1997  39(5):590-4  PubMed ID: 9363658   DOI: 10.1111/j.1442-200x.1997.tb03646.x
2431  Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley, R A, Tsuji A, Matsuda Y  Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.  Infect Immun  1996  64:3093-100  PubMed ID: 8757839   DOI: 10.1128/IAI.64.8.3093-3100.1996


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