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Cell No. : Cell Name
RCB0480 : NB69  update : 2020/09/24
CommentNeuroblastoma. N-myc gene unamplified tumor. Cell growth is slow. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Nishi, Yoshisuke
Originator Gilbert, F.
Year of deposit 1990
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 16 months
Tissue nervous system
Case history neuroblastoma (stage III)
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 15% FBS
Antibiotics Free
Passage method pipetting or 0.25% trypsin (1min.)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 65-94(50) : 82(2),83(1),84(7),85(2),87(1),89(2)/
deposit info
lot info
Reference information Reference 2
User's Publication 10

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1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196
1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592

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User's Publication
11511  Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.  Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.  Nature  2019    PubMed ID: 30971826
11593  Hirano T, Nagasaki-Maeoka E, Ishizuka Y, Takatori A, Watanabe Y, Hoshi R, Yoshizawa S, Kawashima H, Uekusa S, Sugito K, Uehara S, Fukuda N, Nagase H, Takayama T, Soma M, Koshinaga T, Fujiwara K.  Forced expression of NR4A3 induced the differentiation of human neuroblastoma-derived NB1 cells.  Med. Oncol.  2019    PubMed ID: 31183633
4309  Ishizuka Y, Koshinaga T, Hirano T, Nagasaki-Maeoka E, Watanabe Y, Hoshi R, Yoshizawa S, Sugito K, Kawashima H, Uekusa S, Fukuda N, Soma M, Fujiwara K.  NRP1 knockdown promotes the migration and invasion of human neuroblastoma-derived SK‑N‑AS cells via the activation of β1 integrin expression.  Int. J. Oncol.  2018    PubMed ID: 29750423
7960  Wang J, Oue T, Uehara S, Yamanaka H, Oji Y, Fukuzawa M.  The role of WT1 gene in neuroblastoma.  J. Pediatr. Surg.  2011  46:326-31  PubMed ID: 21292082
9586  Kazuko Yamazaki, Emiko Yamada, Yoshio Kanaji, Tetsuo Yanagisawa, Yoshiyuki Kato, Kanji Sato, Kazue Takano, Yuji Sakasegawa, Kiyotoshi Kaneko  Stimulation of Cellular Prion Protein Expression by TSH in Human Thyrocytes  Biochem Biophys Res Commun  2003  305(4):1034-9  PubMed ID: 12767934
2758  Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao  Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.  Jpn J Cancer Res  2002  93:313-9  PubMed ID: 11927014
9022  T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma  The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection  J Gen Virol  2000  81 (Pt 7), 1763-71  PubMed ID: 10859382
2358  Kimura M, Matsuda Y, Yoshioka T, Okano Y  Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.  J Biol Chem  1999  274:7334-40  PubMed ID: 10066797
2182  Kimura M, Kotani S, Hattori T, Sumi N, Yoshioka T, Todokoro K, Okano Y  Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.  J Biol Chem  1997  272:13766-71  PubMed ID: 9153231
2431  Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley, R A, Tsuji A, Matsuda Y  Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.  Infect Immun  1996  64:3093-100  PubMed ID: 8757839

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