Cell No. : Cell Name
RCB0480 : NB69
update : 2021/02/26
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| Comment | Human cell line derived from Neuroblastoma. N-myc gene is not amplified. Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Nishi, Yoshisuke
|
| Originator |
Gilbert, F.
|
| Year of deposit |
1990
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
16 months
|
| Tissue |
nervous system
|
| Disease name |
neuroblastoma (stage III)
|
| Classification |
cancer
|
| History |
Latt, S. A.
|
|
Lifespan |
infinite
|
| Morphology |
other
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
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| Medium and additives |
|
RPMI1640 + 15% FBS
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| Antibiotics |
|
Free
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|
Passage method |
|
pipetting or 0.25% trypsin (1min.)
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Culture information
|
Passage ratio |
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1 : 4 split
|
| SC frequency |
|
Subculture : once/10 days, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
|
| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
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| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
65-94(50) : 82(2),83(1),84(7),85(2),87(1),89(2)/
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
11
|
| User's Publication |
|
11511
Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature
2019
PubMed ID: 30971826
|
|
11593
Hirano T, Nagasaki-Maeoka E, Ishizuka Y, Takatori A, Watanabe Y, Hoshi R, Yoshizawa S, Kawashima H, Uekusa S, Sugito K, Uehara S, Fukuda N, Nagase H, Takayama T, Soma M, Koshinaga T, Fujiwara K.
Forced expression of NR4A3 induced the differentiation of human neuroblastoma-derived NB1 cells.
Med. Oncol.
2019
PubMed ID: 31183633
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12527
Yoda H, Inoue T, Shinozaki Y, Lin J, Watanabe T, Koshikawa N, Takatori A, Nagase H.
Direct Targeting of MYCN Gene Amplification by Site-Specific DNA Alkylation in Neuroblastoma
Cancer Res
2019
79(4):830-840.
PubMed ID: 30584073
|
|
4309
Ishizuka Y, Koshinaga T, Hirano T, Nagasaki-Maeoka E, Watanabe Y, Hoshi R, Yoshizawa S, Sugito K, Kawashima H, Uekusa S, Fukuda N, Soma M, Fujiwara K.
NRP1 knockdown promotes the migration and invasion of human neuroblastoma-derived SK‑N‑AS cells via the activation of β1 integrin expression.
Int. J. Oncol.
2018
PubMed ID: 29750423
|
|
7960
Wang J, Oue T, Uehara S, Yamanaka H, Oji Y, Fukuzawa M.
The role of WT1 gene in neuroblastoma.
J. Pediatr. Surg.
2011
46:326-31
PubMed ID: 21292082
|
|
9586
Kazuko Yamazaki, Emiko Yamada, Yoshio Kanaji, Tetsuo Yanagisawa, Yoshiyuki Kato, Kanji Sato, Kazue Takano, Yuji Sakasegawa, Kiyotoshi Kaneko
Stimulation of Cellular Prion Protein Expression by TSH in Human Thyrocytes
Biochem Biophys Res Commun
2003
305(4):1034-9
PubMed ID: 12767934
|
|
2758
Harada, Hideki, Saijo, Kaoru, Watanabe, Satoru, Tsuboi, Koji, Nose, Tadao, Ishiwata, Isamu, Ohno, Tadao
Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT.
Jpn J Cancer Res
2002
93:313-9
PubMed ID: 11927014
|
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9022
T Suzutani, M Nagamine, T Shibaki, M Ogasawara, I Yoshida, T Daikoku, Y Nishiyama, M Azuma
The Role of the UL41 Gene of Herpes Simplex Virus Type 1 in Evasion of Non-Specific Host Defence Mechanisms During Primary Infection
J Gen Virol
2000
81 (Pt 7), 1763-71
PubMed ID: 10859382
|
|
2358
Kimura M, Matsuda Y, Yoshioka T, Okano Y
Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
J Biol Chem
1999
274:7334-40
PubMed ID: 10066797
|
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2182
Kimura M, Kotani S, Hattori T, Sumi N, Yoshioka T, Todokoro K, Okano Y
Cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, Aik, related to Aurora of Drosophila and yeast Ipl1.
J Biol Chem
1997
272:13766-71
PubMed ID: 9153231
|
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2431
Nagamune H, Ohnishi C, Katsuura A, Fushitani K, Whiley, R A, Tsuji A, Matsuda Y
Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.
Infect Immun
1996
64:3093-100
PubMed ID: 8757839
|
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