Cell No. : Cell Name
RCB0478 : NB16
update : 2024/11/12
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Comment | Human cell line derived from Neuroblastoma. N-myc gene is amplified. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nishi, Yoshisuke
|
Originator |
Gilbert, F.
|
Year of deposit |
1990
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Gender |
Female
|
Age at sampling |
35 months
|
Tissue |
bone marrow
|
Disease name |
neuroblastoma (stage IV)
|
Classification |
cancer
|
History |
Latt, S. A.
|
|
Lifespan |
infinite
|
Morphology |
other
|
Cellosaurus(Expasy) |
CVCL_B323
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture medium |
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RPMI1640 + 15% FBS
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Antibiotics |
|
Free
|
|
Passage method |
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pipetting
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Culture information
|
Passage ratio |
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1 : 2-4 split
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SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
|
5
%
|
Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Virus (HIV) |
|
(-)
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Isozyme |
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LD, NP
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Chromosome mode |
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45-52(50) : 45(2),46(45),47(2),52(1)
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Reference information |
Reference |
3
|
User's Publication |
5
|
Reference |
242
alent A, Le Roux G, Barrois M, Terrier-Lacombe MJ, Valteau-Couanet D, Léon B, Spengler B, Lenoir G, Bénard J, Bernheim A.
MYCN gene overrepresentation detected in primary neuroblastoma tumour cells without amplification
J Pathol
2002
198(4):495-501
PubMed ID: 12434419
DOI: 10.1002/path.1244
|
1287
Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J
Human neuroblastomas and abnormalities of chromosomes 1 and 17.
Cancer Res
1984
44:5444-9
PubMed ID: 6488196
|
1127
Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H
Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.
Cancer Genet Cytogenet
1982
7:33-42
PubMed ID: 7139592
DOI: 10.1016/0165-4608(82)90105-4
|
User's Publication |
16817
Kasahara Y, Shin C, Kubo N, Mihara K, Iwabuchi H, Takachi T, Imamura M, Saitoh A, Imai C.
Development and characterisation of NKp44-based chimeric antigen receptors that confer T cells with NK cell-like specificity
Clin Transl Immunology
2020
9(7):e1147
PubMed ID: 32670576
DOI: 10.1002/cti2.1147
|
4189
Hashizume H, Iijima K, Yamashita K, Kimura T, Wada SI, Sawa R, Igarashi M.
Valgamicin C, a novel cyclic depsipeptide containing the unusual amino acid cleonine, and related valgamicins A, T and V produced by Amycolatopsis sp. ML1-hF4.
J. Antibiot.
2017
PubMed ID: 29138482
DOI: 10.1038/ja.2017.135
|
18785
Sugito K, Kawashima H, Yoshizawa S, Uekusa S, Hoshi R, Furuya T, Kaneda H, Hosoda T, Konuma N, Masuko T, Ohashi K, Ikeda T, Koshinaga T, Tomita R, Shinojima Y, Fujiwara K, Watanabe T, Held WA, Nagase H.
Non-promoter DNA hypermethylation of Zygote Arrest 1 (ZAR1) in neuroblastomas
J Pediatr Surg
2013
48(4):782-8
PubMed ID: 23583134
DOI: 10.1016/j.jpedsurg.2012.08.008
|
2214
Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K
Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.
Cell Struct Funct
1997
22:511-6
PubMed ID: 9431455
DOI: 10.1247/csf.22.511
|
18109
Maeda T, Nishimura N, Nakamura H, Sano K.
p21 (WAF1/Cip1/Sdi1/Pic1) mRNA is expressed in neuroblastoma cell lines but not in Ewing's sarcoma and primitive neuroectodermal tumor cell lines
Acta Paediatr Jpn
1997
39(5):590-4
PubMed ID: 9363658
DOI: 10.1111/j.1442-200x.1997.tb03646.x
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