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Cell No. : Cell Name
RCB0478 : NB16  update : 2023/06/30
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Yoshisuke
Originator Gilbert, F.
Year of deposit 1990
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 35 months
Tissue bone marrow
Disease name neuroblastoma (stage IV)
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_B323
deposit info
lot info
Medium Medium List
Culture medium RPMI1640 + 15% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-52(50) : 45(2),46(45),47(2),52(1)
Reference information Reference 3
User's Publication 5

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242  alent A, Le Roux G, Barrois M, Terrier-Lacombe MJ, Valteau-Couanet D, Léon B, Spengler B, Lenoir G, Bénard J, Bernheim A.   MYCN gene overrepresentation detected in primary neuroblastoma tumour cells without amplification  J Pathol  2002  198(4):495-501  PubMed ID: 12434419   DOI: 10.1002/path.1244
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196  
1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592   DOI: 10.1016/0165-4608(82)90105-4

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User's Publication
16817  Kasahara Y, Shin C, Kubo N, Mihara K, Iwabuchi H, Takachi T, Imamura M, Saitoh A, Imai C.  Development and characterisation of NKp44-based chimeric antigen receptors that confer T cells with NK cell-like specificity  Clin Transl Immunology  2020  9(7):e1147  PubMed ID: 32670576   DOI: 10.1002/cti2.1147
4189  Hashizume H, Iijima K, Yamashita K, Kimura T, Wada SI, Sawa R, Igarashi M.  Valgamicin C, a novel cyclic depsipeptide containing the unusual amino acid cleonine, and related valgamicins A, T and V produced by Amycolatopsis sp. ML1-hF4.  J. Antibiot.  2017    PubMed ID: 29138482   DOI: 10.1038/ja.2017.135
18785  Sugito K, Kawashima H, Yoshizawa S, Uekusa S, Hoshi R, Furuya T, Kaneda H, Hosoda T, Konuma N, Masuko T, Ohashi K, Ikeda T, Koshinaga T, Tomita R, Shinojima Y, Fujiwara K, Watanabe T, Held WA, Nagase H.  Non-promoter DNA hypermethylation of Zygote Arrest 1 (ZAR1) in neuroblastomas  J Pediatr Surg  2013  48(4):782-8  PubMed ID: 23583134   DOI: 10.1016/j.jpedsurg.2012.08.008
2214  Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K  Expression of neural cell adhesion molecule L1 in human lung cancer cell lines.  Cell Struct Funct  1997  22:511-6  PubMed ID: 9431455   DOI: 10.1247/csf.22.511
18109  Maeda T, Nishimura N, Nakamura H, Sano K.  p21 (WAF1/Cip1/Sdi1/Pic1) mRNA is expressed in neuroblastoma cell lines but not in Ewing's sarcoma and primitive neuroectodermal tumor cell lines  Acta Paediatr Jpn  1997  39(5):590-4  PubMed ID: 9363658   DOI: 10.1111/j.1442-200x.1997.tb03646.x

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