| Cell No. : Cell Name RCB0478 : NB16
						
						 update : 2024/11/12
 | 
						
						
						
							
								| Comment | Human cell line derived from Neuroblastoma. N-myc gene is amplified. | 
							
								| Comment from the depositor |  | 
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
								| Remarks |  | 
							
							
								| Order Form | Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										  | 
							
								| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | 
						
						
					
						
						
							
							|  | Basic information | Depositor | Nishi, Yoshisuke | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Originator | Gilbert, F. | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of deposit | 1990 | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Animal | _human
								
								
								
							
								 
								
								
								 	
								 		< Mammals | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Genus | Homo | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Species | sapiens | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Gender | Female | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Age at sampling | 35 months | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Tissue | bone marrow | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Disease name | neuroblastoma (stage IV) | 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Classification | cancer | 
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | History | Latt, S. A. | 
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							 | Lifespan | infinite | 
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | other | 
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Cellosaurus(Expasy) | CVCL_B323 | 
					
									
								
							
						
					
					
					
					
					
					
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
					
						
						
							
							
								|  |  | deposit info | lot info | 
											
												|  | Medium | Medium List | 
										
									
								
						
						
						
						
						
					
						
						
						
						
							 | Culture medium |  | RPMI1640 + 15% FBS | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Antibiotics |  | Free | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							 | Passage method |  | pipetting | Culture information | Passage ratio |  | 1 : 2-4 split | 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| SC frequency |  | Subculture : once/week, Medium Renewal : 2 times/week | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Temperature |  | 37
							
							
						
						
							℃ | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| CO2 concentration |  | 5
							
							
						
						
							% | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freeze medium |  | Medium + 10% DMSO | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freezing method |  | Slow freezing | 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Virus (HIV) |  | (-) | 
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Isozyme |  | LD, NP | 
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Chromosome mode |  | 45-52(50) : 45(2),46(45),47(2),52(1) | 
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
			
					
			
						
						
						
							
								| Reference information | Reference | 3 | 
							
								| User's Publication | 5 | 
						
						
				
			
				
				
				
				
				
					
						| Reference | 
						
							
							| 242 
								alent A, Le Roux G, Barrois M, Terrier-Lacombe MJ, Valteau-Couanet D, Léon B, Spengler B, Lenoir G, Bénard J, Bernheim A.  
								MYCN gene overrepresentation detected in primary neuroblastoma tumour cells without amplification 
								J Pathol 
								2002 
								198(4):495-501 
								
									
										PubMed ID: 12434419
									
								 
								
									
										DOI: 10.1002/path.1244 | 
						
							
							| 1287 
								Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J 
								Human neuroblastomas and abnormalities of chromosomes 1 and 17. 
								Cancer Res 
								1984 
								44:5444-9 
								
									
										PubMed ID: 6488196 | 
						
							
							| 1127 
								Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H 
								Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines. 
								Cancer Genet Cytogenet 
								1982 
								7:33-42 
								
									
										PubMed ID: 7139592
									
								 
								
									
										DOI: 10.1016/0165-4608(82)90105-4 | 
						
					
				
					
						| User's Publication | 
						
							
							| 16817 
								Kasahara Y, Shin C, Kubo N, Mihara K, Iwabuchi H, Takachi T, Imamura M, Saitoh A, Imai C. 
								Development and characterisation of NKp44-based chimeric antigen receptors that confer T cells with NK cell-like specificity 
								Clin Transl Immunology 
								2020 
								9(7):e1147 
								
									
										PubMed ID: 32670576
									
								 
								
									
										DOI: 10.1002/cti2.1147 | 
						
							
							| 4189 
								Hashizume H, Iijima K, Yamashita K, Kimura T, Wada SI, Sawa R, Igarashi M. 
								Valgamicin C, a novel cyclic depsipeptide containing the unusual amino acid cleonine, and related valgamicins A, T and V produced by Amycolatopsis sp. ML1-hF4. 
								J. Antibiot. 
								2017 
								 
								
									
										PubMed ID: 29138482
									
								 
								
									
										DOI: 10.1038/ja.2017.135 | 
						
							
							| 18785 
								Sugito K, Kawashima H, Yoshizawa S, Uekusa S, Hoshi R, Furuya T, Kaneda H, Hosoda T, Konuma N, Masuko T, Ohashi K, Ikeda T, Koshinaga T, Tomita R, Shinojima Y, Fujiwara K, Watanabe T, Held WA, Nagase H. 
								Non-promoter DNA hypermethylation of Zygote Arrest 1 (ZAR1) in neuroblastomas 
								J Pediatr Surg 
								2013 
								48(4):782-8 
								
									
										PubMed ID: 23583134
									
								 
								
									
										DOI: 10.1016/j.jpedsurg.2012.08.008 | 
						
							
							| 2214 
								Katayama M, Iwamatsu A, Masutani H, Furuke K, Takeda K, Wada H, Masuda T, Ishii K 
								Expression of neural cell adhesion molecule L1 in human lung cancer cell lines. 
								Cell Struct Funct 
								1997 
								22:511-6 
								
									
										PubMed ID: 9431455
									
								 
								
									
										DOI: 10.1247/csf.22.511 | 
						
							
							| 18109 
								Maeda T, Nishimura N, Nakamura H, Sano K. 
								p21 (WAF1/Cip1/Sdi1/Pic1) mRNA is expressed in neuroblastoma cell lines but not in Ewing's sarcoma and primitive neuroectodermal tumor cell lines 
								Acta Paediatr Jpn 
								1997 
								39(5):590-4 
								
									
										PubMed ID: 9363658
									
								 
								
									
										DOI: 10.1111/j.1442-200x.1997.tb03646.x |