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Cell No. : Cell Name
RCB0477 : NB9  update : 2018/07/31
CommentNeuroblastoma ( Another name : CHP-212, PMID: 6871857 ) . N-myc gene amplification. Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Nishi, Yoshisuke
Originator Gilbert, F.
Year of deposit 1990
Another name CHP-212
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 22 months
Tissue nervous system
Case history neuroblastoma (stage IV)
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin (1min.) or pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP, AST, MPI
Chromosome mode 42-46(50) : 42(2),43(1),44(4),45(42),46(1)
Images
deposit info
lot info
Reference information Reference 2
User's Publication 2


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Reference
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196
1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592

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User's Publication
4310  Hayashi H, Kubo Y, Izumida M, Takahashi E, Kido H, Sato K, Yamaya M, Nishimura H, Nakayama K, Matsuyama T.  Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines.  Front Cell Infect Microbiol  2018    PubMed ID: 29629340
10457  Watanabe Y, Ishizuka Y, Hirano T, Nagasaki-Maeoka E, Hoshi R, Yoshizawa S, Uekusa S, Kawashima H, Sugito K, Shinohara K, Fukuda N, Nagase H, Soma M, Koshinaga T, Fujiwara K.  ZAR1 knockdown promotes the differentiation of human neuroblastoma cells by suppression of MYCN expression.  Med. Oncol.  2017  34:158  PubMed ID: 28791558



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