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Cell No. : Cell Name
RCB0477 : NB9  update : 2023/09/12
CommentHuman cell line derived from Neuroblastoma. N-myc gene is amplified. Another name is CHP-212 (PMID: 6871857). Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nishi, Yoshisuke
Originator Gilbert, F.
Year of deposit 1990
Another name CHP-212
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 22 months
Tissue nervous system
Disease name neuroblastoma (stage IV)
Classification cancer
History Latt, S. A.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1125
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium RPMI1640 + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin (1min.) or pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, AST, MPI
Chromosome mode 42-46(50) : 42(2),43(1),44(4),45(42),46(1)
STR(human) OK
deposit info
lot info
Reference information Reference 5
User's Publication 7

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242  alent A, Le Roux G, Barrois M, Terrier-Lacombe MJ, Valteau-Couanet D, Léon B, Spengler B, Lenoir G, Bénard J, Bernheim A.   MYCN gene overrepresentation detected in primary neuroblastoma tumour cells without amplification  J Pathol  2002  198(4):495-501  PubMed ID: 12434419   DOI: 10.1002/path.1244
245  Kanda N, Tsuchida Y, Hata J, Kohl NE, Alt FW, Latt SA, Utakoji T.  Amplification of IMR-32 clones 8, G21, and N-myc in human neuroblastoma xenografts  Cancer Res  1987  47(12):3291-5  PubMed ID: 3034408  
1287  Gilbert F, Feder M, Balaban G, Brangman D, Lurie, D K, Podolsky R, Rinaldt V, Vinikoor N, Weisband J  Human neuroblastomas and abnormalities of chromosomes 1 and 17.  Cancer Res  1984  44:5444-9  PubMed ID: 6488196  
1286  U V Santer, M C Glick  Presence of fucosyl residues on the oligosaccharide antennae of membrane glycopeptides of human neuroblastoma cells  Cancer Res  1983  43(9):4159-66  PubMed ID: 6871857  
1127  Gilbert F, Balaban G, Moorhead P, Bianchi D, Schlesinger H  Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.  Cancer Genet Cytogenet  1982  7:33-42  PubMed ID: 7139592   DOI: 10.1016/0165-4608(82)90105-4

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User's Publication
17605  Hidaka A, Uekusa S, Hosokawa T, Kaneda H, Kazama T, Hagikura K, Uehara S, Koshinaga T, Matsumoto T.  Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells  Pediatr Surg Int  2022  39(1):58  PubMed ID: 36547710   DOI: 10.1007/s00383-022-05304-x
13941  Nagasaki-Maeoka E, Ikeda K, Takayama KI, Hirano T, Ishizuka Y, Koshinaga T, Tsukune N, Takayama T, Inoue S, Fujiwara K.  Polyethylene glycol derivative 9bw suppresses growth of neuroblastoma cells by inhibiting oxidative phosphorylation  Cancer Sci  2020  111(8):2943-2953  PubMed ID: 32495467   DOI: 10.1111/cas.14512
4310  Hayashi H, Kubo Y, Izumida M, Takahashi E, Kido H, Sato K, Yamaya M, Nishimura H, Nakayama K, Matsuyama T.  Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines.  Front Cell Infect Microbiol  2018    PubMed ID: 29629340   DOI: 10.3389/fcimb.2018.00091
10457  Watanabe Y, Ishizuka Y, Hirano T, Nagasaki-Maeoka E, Hoshi R, Yoshizawa S, Uekusa S, Kawashima H, Sugito K, Shinohara K, Fukuda N, Nagase H, Soma M, Koshinaga T, Fujiwara K.  ZAR1 knockdown promotes the differentiation of human neuroblastoma cells by suppression of MYCN expression.  Med. Oncol.  2017  34:158  PubMed ID: 28791558   DOI: 10.1007/s12032-017-0999-x
14441  Hoshi R, Watanabe Y, Ishizuka Y, Hirano T, Nagasaki-Maeoka E, Yoshizawa S, Uekusa S, Kawashima H, Ohashi K, Sugito K, Fukuda N, Nagase H, Soma M, Ozaki T, Koshinaga T, Fujiwara K.  Depletion of TFAP2E attenuates adriamycin-mediated apoptosis in human neuroblastoma cells  Oncol Rep  2017  37(4):2459-2464  PubMed ID: 28260105   DOI: 10.3892/or.2017.5477
14739  Takagi M, Yoshida M, Nemoto Y, Tamaichi H, Tsuchida R, Seki M, Uryu K, Nishii R, Miyamoto S, Saito M, Hanada R, Kaneko H, Miyano S, Kataoka K, Yoshida K, Ohira M, Hayashi Y, Nakagawara A, Ogawa S, Mizutani S, Takita J.  Loss of DNA Damage Response in Neuroblastoma and Utility of a PARP Inhibitor  J Natl Cancer Inst  2017  109(11)  PubMed ID: 29059438   DOI: 10.1093/jnci/djx062
18109  Maeda T, Nishimura N, Nakamura H, Sano K.  p21 (WAF1/Cip1/Sdi1/Pic1) mRNA is expressed in neuroblastoma cell lines but not in Ewing's sarcoma and primitive neuroectodermal tumor cell lines  Acta Paediatr Jpn  1997  39(5):590-4  PubMed ID: 9363658   DOI: 10.1111/j.1442-200x.1997.tb03646.x

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