Cell No. : Cell Name
RCB0476 : Had-1
update : 2024/01/17
|
| Comment | A subline of FM3A (mouse breast cancer cell line). Resistant to Newcastle virus and Sendai virus. Dye by GS-II lectin. The revertant cells (parent cell-like cells) dye by WGA lectin. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Kawakita, Masao
|
| Originator |
Kawakita, Masao
|
| Year of deposit |
1990
|
| Original cell |
FM3A
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/HeN
|
| Gender |
Female
|
| Tissue |
mammary
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_J854
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
ES + 2% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP, G6PD
|
| Reference information |
Reference |
6
|
| User's Publication |
0
|
| Reference |
|
236
Ishida N, Yoshioka S, Iida M, Sudo K, Miura N, Aoki K, Kawakita M.
Indispensability of transmembrane domains of Golgi UDP-galactose transporter as revealed by analysis of genetic defects in UDP-galactose transporter-deficient murine had-1 mutant cell lines and construction of deletion mutants
J Biochem
1999
126(6):1107-17.
PubMed ID: 10578063
DOI: 10.1093/oxfordjournals.jbchem.a022556
|
|
235
Yoshioka S, Sun-Wada GH, Ishida N, Kawakita M.
Expression of the human UDP-galactose transporter in the Golgi membranes of murine Had-1 cells that lack the endogenous transporter
J Biochem
1997
122(4):691-5
PubMed ID: 9399569
DOI: 10.1093/oxfordjournals.jbchem.a021810
|
|
241
Miura N, Ishida N, Hoshino M, Yamauchi M, Hara T, Ayusawa D, Kawakita M.
Human UDP-galactose translocator: molecular cloning of a complementary DNA that complements the genetic defect of a mutant cell line deficient in UDP-galactose translocator
J Biochem
1996
120(2):236-41
PubMed ID: 8889805
DOI: 10.1093/oxfordjournals.jbchem.a021404
|
|
240
Taki T, Ogura K, Rokukawa C, Hara T, Kawakita M, Endo T, Kobata A, Handa S.
Had-1, a uridine 5'-diphosphogalactose transport-defective mutant of mouse mammary tumor cell FM3A: composition of glycolipids, cell growth inhibition by lactosylceramide, and loss of tumorigenicity
Cancer Res
1991
51(6):1701-7
PubMed ID: 1998961
|
|
1196
Hara T, Hattori S, Kawakita M
Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease virus receptors.
J Virol
1989
63:182-8
PubMed ID: 2535724
DOI: 10.1128/JVI.63.1.182-188.1989
|
|
1119
Hara T, Endo T, Furukawa K, Kawakita M, Kobata A
Elucidation of the Phenotypic Change on the Surface of Had-1 Cell, a Mutant Cell Line of Mouse FM3A Carcinoma Cells Selected by Resistance to Newcastle Disease Virus Infection.
J Biochem (Tokyo)
1989
106:236-247
PubMed ID: 2808320
DOI: 10.1093/oxfordjournals.jbchem.a122838
|
Back
Top
CELL BANK Website