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Cell No. : Cell Name
RCB0476 : Had-1  update : 2024/01/17
CommentA subline of FM3A (mouse breast cancer cell line). Resistant to Newcastle virus and Sendai virus. Dye by GS-II lectin. The revertant cells (parent cell-like cells) dye by WGA lectin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawakita, Masao
Originator Kawakita, Masao
Year of deposit 1990
Original cell FM3A
Animal _mouse < Mammals
Strain name C3H/HeN
Gender Female
Tissue mammary
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J854
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium ES + 2% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Reference information Reference 6
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Reference
236  Ishida N, Yoshioka S, Iida M, Sudo K, Miura N, Aoki K, Kawakita M.  Indispensability of transmembrane domains of Golgi UDP-galactose transporter as revealed by analysis of genetic defects in UDP-galactose transporter-deficient murine had-1 mutant cell lines and construction of deletion mutants  J Biochem  1999  126(6):1107-17.  PubMed ID: 10578063   DOI: 10.1093/oxfordjournals.jbchem.a022556
235  Yoshioka S, Sun-Wada GH, Ishida N, Kawakita M.  Expression of the human UDP-galactose transporter in the Golgi membranes of murine Had-1 cells that lack the endogenous transporter  J Biochem  1997  122(4):691-5  PubMed ID: 9399569   DOI: 10.1093/oxfordjournals.jbchem.a021810
241  Miura N, Ishida N, Hoshino M, Yamauchi M, Hara T, Ayusawa D, Kawakita M.  Human UDP-galactose translocator: molecular cloning of a complementary DNA that complements the genetic defect of a mutant cell line deficient in UDP-galactose translocator  J Biochem  1996  120(2):236-41  PubMed ID: 8889805   DOI: 10.1093/oxfordjournals.jbchem.a021404
240  Taki T, Ogura K, Rokukawa C, Hara T, Kawakita M, Endo T, Kobata A, Handa S.  Had-1, a uridine 5'-diphosphogalactose transport-defective mutant of mouse mammary tumor cell FM3A: composition of glycolipids, cell growth inhibition by lactosylceramide, and loss of tumorigenicity  Cancer Res  1991  51(6):1701-7  PubMed ID: 1998961  
1196  Hara T, Hattori S, Kawakita M  Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease virus receptors.  J Virol  1989  63:182-8  PubMed ID: 2535724   DOI: 10.1128/JVI.63.1.182-188.1989
1119  Hara T, Endo T, Furukawa K, Kawakita M, Kobata A  Elucidation of the Phenotypic Change on the Surface of Had-1 Cell, a Mutant Cell Line of Mouse FM3A Carcinoma Cells Selected by Resistance to Newcastle Disease Virus Infection.  J Biochem (Tokyo)  1989  106:236-247  PubMed ID: 2808320   DOI: 10.1093/oxfordjournals.jbchem.a122838

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