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Cell No. : Cell Name
RCB0476 : Had-1  update : 2022/03/15
CommentA subline of FM3A (mouse breast cancer cell line). Resistant to Newcastle virus and Sendai virus. Dye by GS-II lectin. The revertant cells (parent cell-like cells) dye by WGA lectin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawakita, Masao
Originator Kawakita, Masao
Year of deposit 1990
Original cell FM3A
Animal mouse < Mammals
Strain name C3H/HeN
Gender Female
Tissue mammary
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_J854
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives ES + 2% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, G6PD
Reference information Reference 2
User's Publication 0


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Reference
1196  Hara T, Hattori S, Kawakita M  Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease virus receptors.  J Virol  1989  63:182-8  PubMed ID: 2535724  
1119  Hara T, Endo T, Furukawa K, Kawakita M, Kobata A  Elucidation of the Phenotypic Change on the Surface of Had-1 Cell, a Mutant Cell Line of Mouse FM3A Carcinoma Cells Selected by Resistance to Newcastle Disease Virus Infection.  J Biochem (Tokyo)  1989  106:236-247  PubMed ID: 2808320   DOI: 10.1093/oxfordjournals.jbchem.a122838

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