細胞番号 : 細胞名
RCB0475 : ILT-Mat
update : 2024/10/02
|
| 細胞特性(Comment:英) | BSL2.IL-2 dependent ATL cell line. Based on the information by depositor; this cell line possesses HTLV-1 pro-virus DNA. We don't know whether virus particles are produced or not. |
| 細胞特性(日) | BSL2. IL-2依存性ヒトATL細胞、HTLV-1 pro-virus DNA(+)。表面抗原はCD2, CD4, Ia, IL-2R陽性、CD3, CD8陰性。ウイルス産生の有無は不明です。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
BSL2誓約書C-0069.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Sugamura, Kazuo
|
| 樹立者 |
Sugamura, Kazuo
|
| 寄託日 |
1990
|
| 元の細胞 |
TKG0286
|
| 動物種 |
_human
< Mammals
|
| 属名 |
Homo
|
| 種名 |
sapiens
|
| 人種 |
Japanese
|
| 性別 |
Male
|
| 採取組織 |
blood
|
| 病名 |
ATL (acute T cell leukemia)
|
| 細胞分類 |
cancer
|
| 入手歴 |
Decontaminated myco. by originator
|
| メモ_1 |
Mycoplasma was eliminated with MC-210 by the depositor.
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
lymphocyte-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_A782
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Suspension cells
|
| 培養方法 |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| 培地 |
|
RPMI1640 + 10% FBS + 200U/ml human IL-2
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
dilution
|
|
継代密度 |
|
1 : 10 split
|
| 継代・培地交換頻度 |
|
Subculture : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ/アコレプラズマ |
|
(-)
|
| ウィルス（HIV) |
|
(-)
|
| ウィルス（HTLV-1) |
|
DNA (+)
|
| 個体識別検査 |
|
OK
|
| 文献情報 |
Reference(英) |
9件
|
| Reference(日) |
0件
|
| 利用者成果(英) |
4件
|
| 利用者成果(日) |
0件
|
| Reference(英) |
|
862
Chen S, Ishii N, Ine S, Ikeda S, Fujimura T, Ndhlovu LC, Soroosh P, Tada K, Harigae H, Kameoka J, Kasai N, Sasaki T, Sugamura K.
Regulatory T cell-like activity of Foxp3+ adult T cell leukemia cells
Int Immunol
2006
18(2):269-77
PubMed ID: 16361311
DOI: 10.1093/intimm/dxh366
|
|
228
Takeshita T, Arita T, Higuchi M, Asao H, Endo K, Kuroda H, Tanaka N, Murata K, Ishii N, Sugamura K.
STAM, signal transducing adaptor molecule, is associated with Janus kinases and involved in signaling for cell growth and c-myc induction
Immunity
1997
6(4):449-57.
PubMed ID: 9133424
DOI: 10.1016/s1074-7613(00)80288-5
|
|
234
Imada K, Takaori-Kondo A, Akagi T, Shimotohno K, Sugamura K, Hattori T, Yamabe H, Okuma M, Uchiyama T.
Tumorigenicity of human T-cell leukemia virus type I-infected cell lines in severe combined immunodeficient mice and characterization of the cells proliferating in vivo
Blood
1995
86(6):2350-7
PubMed ID: 7662981
|
|
229
Tanaka N, Asao H, Ohbo K, Ishii N, Takeshita T, Nakamura M, Sasaki H, Sugamura K.
Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor beta and gamma chains
Proc Natl Acad Sci U S A
1994
91(15):7271-5
PubMed ID: 8041779
DOI: 10.1073/pnas.91.15.7271
|
|
232
Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K.
A novel human tyrosine kinase gene inducible in T cells by interleukin 2
FEBS Lett
1993
324(1):1-5
PubMed ID: 8504851
DOI: 10.1016/0014-5793(93)81520-a
|
|
233
Takeshita T, Ohbo K, Nakamura M, Goto Y, Sugamura K.
Establishment of a cyclic adenosine monophosphate-dependent growing human T-cell line derived from an interleukin-2-dependent cell line
J Cell Physiol
1990
145(2):238-43
PubMed ID: 2174062
DOI: 10.1002/jcp.1041450207
|
|
1115
Takeshita T, Goto Y, Tada K, Nagata K, Asao H, Sugamura K
Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.
J Exp Med
1989
169:1323-32
PubMed ID: 2784485
DOI: 10.1084/jem.169.4.1323
|
|
231
Takeshita T, Goto Y, Nakamura M, Fujii M, Iwami M, Hinuma Y, Sugamura K.
Phorbol esters can persistently replace interleukin-2 (IL-2) for the growth of a human IL-2-dependent T-cell line
J Cell Physiol
1988
136(2):319-25
PubMed ID: 3261728
DOI: 10.1002/jcp.1041360215
|
|
230
Ishii T, Kohno M, Nakamura M, Hinuma Y, Sugamura K.
Characterization of interleukin 2-stimulated phosphorylation of 67 and 63 kDa proteins in human T-cells
Biochem J
1987
242(1):211-9
PubMed ID: 3496080
DOI: 10.1042/bj2420211
|
| 利用者成果(英) |
|
21989
Sudo H, Tonoyama Y, Ikebe E, Hasegawa H, Iha H, Ishida YI.
Proteomic analysis of adult T-cell leukemia/lymphoma: A biomarker identification strategy based on preparation and in-solution digestion methods of total proteins
Leuk Res
2024
138:107454
PubMed ID: 38452534
DOI: 10.1016/j.leukres.2024.107454
|
|
13937
Mitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguchi S, Norimine J.
Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
J Vet Med Sci
2021
83(1):134-141
PubMed ID: 33177289
DOI: 10.1292/jvms.20-0423
|
|
10693
Tatsuro Watanabe, Satoshi Yamashita, Hiroshi Ureshino, Kazuharu Kamachi, Yuki Kurahashi, Yuki Fukuda-Kurahashi, Nao Yoshida, Naoko Hattori, Hideaki Nakamura, Akemi Sato, Atsushi Kawaguchi, Naoko Sueoka-Aragane, Kensuke Kojima, Seiji Okada, Toshikazu Ushijima, Shinya Kimura, Eisaburo Sueoka
Targeting Aberrant DNA Hypermethylation as a Driver of ATL Leukemogenesis Using the New Oral Demethylating Agent OR-2100
Blood
2020
136(7):871-884
PubMed ID: 32391874
DOI: 10.1182/blood.2019003084
|
|
2639
Oka, Takashi, Ouchida, Mamoru, Koyama, Maho, Ogama, Yoichiro, Takada, Shinichi, Nakatani, Yoko, Tanaka, Takehiro, Yoshino, Tadashi, Hayashi, Kazuhiko, Ohara, Nobuya, Kondo, Eisaku, Takahashi, Kiyoshi, Tsuchiyama, Junjiro, Tanimoto, Mitsune, Shimizu, Kenji, Akagi, Tadaatsu
Gene silencing of the tyrosine phosphatase SHP1 gene by aberrant methylation in leukemias/lymphomas.
Cancer Res
2002
62:6390-4
PubMed ID: 12438221
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト