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Cell No. : Cell Name
RCB0475 : ILT-Mat  update : 2024/10/02
CommentBSL2.IL-2 dependent ATL cell line. Based on the information by depositor; this cell line possesses HTLV-1 pro-virus DNA. We don't know whether virus particles are produced or not.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugamura, Kazuo
Originator Sugamura, Kazuo
Year of deposit 1990
Original cell TKG0286
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue blood
Disease name ATL (acute T cell leukemia)
Classification cancer
History Decontaminated myco. by originator
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology lymphocyte-like
BSL BSL2
Cellosaurus(Expasy) CVCL_A782
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + 200U/ml human IL-2
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) DNA (+)
STR(human) OK
Reference information Reference 9
User's Publication 4


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Reference
862  Chen S, Ishii N, Ine S, Ikeda S, Fujimura T, Ndhlovu LC, Soroosh P, Tada K, Harigae H, Kameoka J, Kasai N, Sasaki T, Sugamura K.  Regulatory T cell-like activity of Foxp3+ adult T cell leukemia cells  Int Immunol  2006  18(2):269-77  PubMed ID: 16361311   DOI: 10.1093/intimm/dxh366
228  Takeshita T, Arita T, Higuchi M, Asao H, Endo K, Kuroda H, Tanaka N, Murata K, Ishii N, Sugamura K.  STAM, signal transducing adaptor molecule, is associated with Janus kinases and involved in signaling for cell growth and c-myc induction  Immunity  1997  6(4):449-57.  PubMed ID: 9133424   DOI: 10.1016/s1074-7613(00)80288-5
234  Imada K, Takaori-Kondo A, Akagi T, Shimotohno K, Sugamura K, Hattori T, Yamabe H, Okuma M, Uchiyama T.  Tumorigenicity of human T-cell leukemia virus type I-infected cell lines in severe combined immunodeficient mice and characterization of the cells proliferating in vivo  Blood  1995  86(6):2350-7  PubMed ID: 7662981  
229  Tanaka N, Asao H, Ohbo K, Ishii N, Takeshita T, Nakamura M, Sasaki H, Sugamura K.  Physical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor beta and gamma chains  Proc Natl Acad Sci U S A  1994  91(15):7271-5  PubMed ID: 8041779   DOI: 10.1073/pnas.91.15.7271
232  Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K.   A novel human tyrosine kinase gene inducible in T cells by interleukin 2  FEBS Lett  1993  324(1):1-5  PubMed ID: 8504851   DOI: 10.1016/0014-5793(93)81520-a
233  Takeshita T, Ohbo K, Nakamura M, Goto Y, Sugamura K.  Establishment of a cyclic adenosine monophosphate-dependent growing human T-cell line derived from an interleukin-2-dependent cell line  J Cell Physiol  1990  145(2):238-43  PubMed ID: 2174062   DOI: 10.1002/jcp.1041450207
1115  Takeshita T, Goto Y, Tada K, Nagata K, Asao H, Sugamura K  Monoclonal antibody defining a molecule possibly identical to the p75 subunit of interleukin 2 receptor.  J Exp Med  1989  169:1323-32  PubMed ID: 2784485   DOI: 10.1084/jem.169.4.1323
231  Takeshita T, Goto Y, Nakamura M, Fujii M, Iwami M, Hinuma Y, Sugamura K.  Phorbol esters can persistently replace interleukin-2 (IL-2) for the growth of a human IL-2-dependent T-cell line  J Cell Physiol  1988  136(2):319-25  PubMed ID: 3261728   DOI: 10.1002/jcp.1041360215
230  Ishii T, Kohno M, Nakamura M, Hinuma Y, Sugamura K.  Characterization of interleukin 2-stimulated phosphorylation of 67 and 63 kDa proteins in human T-cells  Biochem J  1987  242(1):211-9  PubMed ID: 3496080   DOI: 10.1042/bj2420211

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User's Publication
21989  Sudo H, Tonoyama Y, Ikebe E, Hasegawa H, Iha H, Ishida YI.  Proteomic analysis of adult T-cell leukemia/lymphoma: A biomarker identification strategy based on preparation and in-solution digestion methods of total proteins  Leuk Res  2024  138:107454  PubMed ID: 38452534   DOI: 10.1016/j.leukres.2024.107454
13937  Mitoma S, El-Khaiat HM, Uto T, Sato K, Sekiguchi S, Norimine J.  Characterization of bovine interleukin-2 stably expressed in HEK-293 cells  J Vet Med Sci  2021  83(1):134-141  PubMed ID: 33177289   DOI: 10.1292/jvms.20-0423
10693  Tatsuro Watanabe, Satoshi Yamashita, Hiroshi Ureshino, Kazuharu Kamachi, Yuki Kurahashi, Yuki Fukuda-Kurahashi, Nao Yoshida, Naoko Hattori, Hideaki Nakamura, Akemi Sato, Atsushi Kawaguchi, Naoko Sueoka-Aragane, Kensuke Kojima, Seiji Okada, Toshikazu Ushijima, Shinya Kimura, Eisaburo Sueoka  Targeting Aberrant DNA Hypermethylation as a Driver of ATL Leukemogenesis Using the New Oral Demethylating Agent OR-2100  Blood  2020  136(7):871-884  PubMed ID: 32391874   DOI: 10.1182/blood.2019003084
2639  Oka, Takashi, Ouchida, Mamoru, Koyama, Maho, Ogama, Yoichiro, Takada, Shinichi, Nakatani, Yoko, Tanaka, Takehiro, Yoshino, Tadashi, Hayashi, Kazuhiko, Ohara, Nobuya, Kondo, Eisaku, Takahashi, Kiyoshi, Tsuchiyama, Junjiro, Tanimoto, Mitsune, Shimizu, Kenji, Akagi, Tadaatsu  Gene silencing of the tyrosine phosphatase SHP1 gene by aberrant methylation in leukemias/lymphomas.  Cancer Res  2002  62:6390-4  PubMed ID: 12438221  


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