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Cell No. : Cell Name
RCB0474 : K562/MTX-2  update : 2022/03/15
CommentMethotrexate(MTX)-resistant K562 cell line
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Kanazawa University
Graduate School of Medical Science Angiogenesis and Vascular Development (Department of Pediatrics)
13-1 Takaramachi, Kanazawa, Ishikawa 920-8640 ,Japan
Dr.KOIZUMI Shoichi
Fax. +81-762-62-1866
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Koizumi, Shoichi
Originator Koizumi, Shoichi
Year of deposit 1990
Original cell K562
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue pleural effusion
Classification cancer
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_5144
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 2x10 -6M MTX
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 44-61(50) : 55(2),56(3),57(19),58(23),59(2),61(1)
STR(human) OK
Reference information Reference 7
User's Publication 0

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227  Koizumi S, Allegra CJ.   Enzyme studies of methotrexate-resistant human leukemic cell (K562) subclones  Leuk Res  1992  16(6-7):565-9  PubMed ID: 1378915   DOI: 10.1016/0145-2126(92)90003-p
1116  Koizumi S  Impairment of Methotrexate(MTX)-Polyglutamate Formation of MTX-resistant K562 Cell Lines.  Jpn J Cancer Res  1988  79:1230-1237  PubMed ID: 2465288   DOI: 10.1111/j.1349-7006.1988.tb01549.x
87  Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.  A multipotential leukemia cell line (K-562) of human origin  Proc Soc Exp Biol Med  1981  166(4):546-50  PubMed ID: 7194480   DOI: 10.3181/00379727-166-41106
4603  Lozzio BB, Lozzio CB.  Properties and usefulness of the original K-562 human myelogenous leukemia cell line.  Leuk Res  1979  3(6):363-70  PubMed ID: 95026   DOI: 10.1016/0145-2126(79)90033-x
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354   DOI: 10.1038/280164a0
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644   DOI: 10.1038/278364a0
4602  Lozzio CB, Lozzio BB.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood  1975  45(3):321-34  PubMed ID: 163658  

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