Cell No. : Cell Name RCB0471 : CYNOM-K1  update : 2024/11/14
Comment			CITES. Cynomolgus monkey embryo skin, early passage.Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Mukai,R. & Ohno,T.
		Originator	Kurita, Kaoru
		Year of deposit	1990
		Animal	_monkey < Mammals
		Strain name	cynomolgus
		Genus	Macaca
		Species	fascicularis
		Age at sampling	80 days embryo
		Tissue	embryo/fetus, skin
		Classification	normal
		Year of origin	1990
		Lifespan	unknown
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_4157
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM ALPHA + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 1-2 times/week, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		35-84(50) : /38(1),39(1),40(5),41(13),42(25),43(2)/
Reference information		Reference	6
		User's Publication	1

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Reference

867  Sii-Felice K, Castillo Padilla J, Relouzat F,  Enhanced Transduction of Macaca fascicularis Hematopoietic Cells with Chimeric Lentiviral Vectors  Hum Gene Ther  2019  30(10):1306-1323  PubMed ID: 30848170   DOI: 10.1089/hum.2018.179

868  Dotti S, Lombardo T, Villa R, Cacciamali A, Zanotti C, Andreani NA, Cinotti S, Ferrari M.  Transformation and Tumorigenicity Testing of Simian Cell Lines and Evaluation of Poliovirus Replication  PLoS One  2017  12(1):e0169391  PubMed ID: 28046048   DOI: 10.1371/journal.pone.0169391

866  Cordy JC, Morley PJ, Wright TJ, Birchler MA, Lewis AP, Emmins R, Chen YZ, Powley WM, Bareille PJ, Wilson R, Tonkyn J, Bayliffe AI, Lazaar AL.  Specificity of human anti-variable heavy (VH ) chain autoantibodies and impact on the design and clinical testing of a VH domain antibody antagonist of tumour necrosis factor-α receptor 1  Clin Exp Immunol  2015  182(2):139-48  PubMed ID: 26178412   DOI: 10.1111/cei.12680

863  Cagliani R, Forni D, Biasin M, Comabella M, Guerini FR, Riva S, Pozzoli U, Agliardi C, Caputo D, Malhotra S, Montalban X, Bresolin N, Clerici M, Sironi M.  Ancient and recent selective pressures shaped genetic diversity at AIM2-like nucleic acid sensors  Genome Biol Evol  2014  6(4):830-45  PubMed ID: 24682156   DOI: 10.1093/gbe/evu066

865  Casals F, Ferrer-Admetlla A, Chillarón J, Torrents D, Palacín M, Bertranpetit J.  Is there selection for the pace of successive inactivation of the arpAT gene in primates?  J Mol Evol  2008  67(1):23-8  PubMed ID: 18566733   DOI: 10.1007/s00239-008-9120-6

864  Takeuchi Y, Patience C, Magre S, Weiss RA, Banerjee PT, Le Tissier P, Stoye JP.  Host range and interference studies of three classes of pig endogenous retrovirus  J Virol  1998  72(12):9986-91  PubMed ID: 9811736   DOI: 10.1128/JVI.72.12.9986-9991.1998

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User's Publication

9828  Tabata K, Sugano E, Murakami F, Yamashita T, Ozaki T, Tomita H.  Improved transduction efficiencies of adeno-associated virus vectors by synthetic cell-permeable peptides.  Biochem. Biophys. Res. Commun.  2016  478:1732-8  PubMed ID: 27614311   DOI: 10.1016/j.bbrc.2016.09.014