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Cell No. : Cell Name
RCB0471 : CYNOM-K1  update : 2022/08/08
CommentCynomolgus monkey embryo skin, early passage.Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mukai,R. & Ohno,T.
Originator Kurita, Kaoru
Year of deposit 1990
Animal monkey < Mammals
Strain name cynomolgus
Genus Macaca
Species fascicularis
Age at sampling 80 days embryo
Tissue embryo/fetus, skin
Classification normal
Year of origin 1990
Lifespan unknown
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4157
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 35-84(50) : /38(1),39(1),40(5),41(13),42(25),43(2)/
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
9828  Tabata K, Sugano E, Murakami F, Yamashita T, Ozaki T, Tomita H.  Improved transduction efficiencies of adeno-associated virus vectors by synthetic cell-permeable peptides.  Biochem. Biophys. Res. Commun.  2016  478:1732-8  PubMed ID: 27614311   DOI: 10.1016/j.bbrc.2016.09.014



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