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Cell No. : Cell Name
RCB0471 : CYNOM-K1  update : 2022/12/07
CommentCITES. Cynomolgus monkey embryo skin, early passage.Monkey cell lines require import and export permits according to the Convention on International Trade in Endangered Species (CITES).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mukai,R. & Ohno,T.
Originator Kurita, Kaoru
Year of deposit 1990
Animal _monkey < Mammals
Strain name cynomolgus
Genus Macaca
Species fascicularis
Age at sampling 80 days embryo
Tissue embryo/fetus, skin
Classification normal
Year of origin 1990
Lifespan unknown
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4157
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 35-84(50) : /38(1),39(1),40(5),41(13),42(25),43(2)/
Reference information Reference 6
User's Publication 1

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867  Sii-Felice K, Castillo Padilla J, Relouzat F,  Enhanced Transduction of Macaca fascicularis Hematopoietic Cells with Chimeric Lentiviral Vectors  Hum Gene Ther  2019  30(10):1306-1323  PubMed ID: 30848170   DOI: 10.1089/hum.2018.179
868  Dotti S, Lombardo T, Villa R, Cacciamali A, Zanotti C, Andreani NA, Cinotti S, Ferrari M.  Transformation and Tumorigenicity Testing of Simian Cell Lines and Evaluation of Poliovirus Replication  PLoS One  2017  12(1):e0169391  PubMed ID: 28046048   DOI: 10.1371/journal.pone.0169391
866  Cordy JC, Morley PJ, Wright TJ, Birchler MA, Lewis AP, Emmins R, Chen YZ, Powley WM, Bareille PJ, Wilson R, Tonkyn J, Bayliffe AI, Lazaar AL.  Specificity of human anti-variable heavy (VH ) chain autoantibodies and impact on the design and clinical testing of a VH domain antibody antagonist of tumour necrosis factor-α receptor 1  Clin Exp Immunol  2015  182(2):139-48  PubMed ID: 26178412   DOI: 10.1111/cei.12680
863  Cagliani R, Forni D, Biasin M, Comabella M, Guerini FR, Riva S, Pozzoli U, Agliardi C, Caputo D, Malhotra S, Montalban X, Bresolin N, Clerici M, Sironi M.  Ancient and recent selective pressures shaped genetic diversity at AIM2-like nucleic acid sensors  Genome Biol Evol  2014  6(4):830-45  PubMed ID: 24682156   DOI: 10.1093/gbe/evu066
865  Casals F, Ferrer-Admetlla A, Chillarón J, Torrents D, Palacín M, Bertranpetit J.  Is there selection for the pace of successive inactivation of the arpAT gene in primates?  J Mol Evol  2008  67(1):23-8  PubMed ID: 18566733   DOI: 10.1007/s00239-008-9120-6
864  Takeuchi Y, Patience C, Magre S, Weiss RA, Banerjee PT, Le Tissier P, Stoye JP.  Host range and interference studies of three classes of pig endogenous retrovirus  J Virol  1998  72(12):9986-91  PubMed ID: 9811736   DOI: 10.1128/JVI.72.12.9986-9991.1998

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User's Publication
9828  Tabata K, Sugano E, Murakami F, Yamashita T, Ozaki T, Tomita H.  Improved transduction efficiencies of adeno-associated virus vectors by synthetic cell-permeable peptides.  Biochem. Biophys. Res. Commun.  2016  478:1732-8  PubMed ID: 27614311   DOI: 10.1016/j.bbrc.2016.09.014

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