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Cell No. : Cell Name
RCB0457 : BM-N  update : 2024/06/03
CommentUseful for recombinant BmNPV expression. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Horiuchi, Tadashi
Year of deposit 1990
Animal _silkworm < Insect
Genus Bombyx
Species mori
Gender Female
Tissue ovary
Classification other
History Maeda, Susumu (Univ. Calif.)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_Z633
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium TC-10 (or TC-100) + 10% FBS (heat inactivated)
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 132-363(50) : /291(2),292(2),294(1),295(2),298(3)/
deposit info
lot info
Reference information Reference 4
User's Publication 4

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4684  Sekine H, Fuse A, Tada A, Maeda S, Simizu B.  Expression of human papillomavirus type 6b E2 gene product with DNA-binding activity in insect (Bombyx mori) cells using a baculovirus expression vector.  Gene  1988  65(2):187-93  PubMed ID: 2842232   DOI: 10.1016/0378-1119(88)90455-6
4683  Marumoto Y, Sato Y, Fujiwara H, Sakano K, Saeki Y, Agata M, Furusawa M, Maeda S.  Hyperproduction of polyhedrin-IGF II fusion protein in silkworm larvae infected with recombinant Bombyx mori nuclear polyhedrosis virus.  J Gen Virol  1987  68 ( Pt 10):2599-606  PubMed ID: 3312489   DOI: 10.1099/0022-1317-68-10-2599
4681  Maeda S, Kawai T, Obinata M, Fujiwara H, Horiuchi T, Saeki Y, Sato Y, Furusawa M.  Production of human alpha-interferon in silkworm using a baculovirus vector.  Nature  1985  315(6020):592-4  PubMed ID: 2989694   DOI: 10.1038/315592a0
4682  Volkman LE1, Goldsmith PA.  Generalized Immunoassay for Autographa californica Nuclear Polyhedrosis Virus Infectivity In Vitro.  Appl Environ Microbiol  1982  44(1):227-33  PubMed ID: 16346059   DOI: 10.1128/aem.44.1.227-233.1982

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User's Publication
20867  Homareda H, Suga K, Yamamoto-Hijikata S, Eishi Y, Ushimaru M, Hara Y.  β subunit affects Na+ and K+ affinities of Na+/K+-ATPase: Na+ and K+ affinities of a hybrid Na+/K+-ATPase composed of insect α and mammalian β subunits.  Biochem Biophys Rep  2022  32:101347  PubMed ID: 36131851   DOI: 10.1016/j.bbrep.2022.101347
10566  Homareda H, Otsu M, Yamamoto S, Ushimaru M, Ito S, Fukutomi T, Jo T, Eishi Y, Hara Y.  A possible mechanism for low affinity of silkworm Na+/K+-ATPase for K.  J. Bioenerg. Biomembr.  2017  49:463-472  PubMed ID: 29047027   DOI: 10.1007/s10863-017-9729-5
7162  Kayukawa T, Nagamine K, Ito Y, Nishita Y, Ishikawa Y, Shinoda T.  Kruppel Homolog 1 Inhibits Insect Metamorphosis via Direct Transcriptional Repression of Broad-Complex, a Pupal Specifier Gene.  J. Biol. Chem.  2016  291:1751-62  PubMed ID: 26518872   DOI: 10.1074/jbc.M115.686121
8687  Yoshinori Nishita and Shigeharu Takiya  Differential responsiveness of BmBR-C promoters to ecdysone signals  J Insect Biotech Sericol.  2009  78:127-138    DOI: 10.11416/jibs.78.3_12

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