Cell No. : Cell Name
RCB0453 : SHOK
update : 2024/11/14
|
| Comment | Contact-inhibited hamster embryonal cell line |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Miyoshi, Jun
|
| Originator |
Azuma, T. & Suzuki, F.
|
| Year of deposit |
1989
|
| Another name |
GHE-c8
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Syrian
|
| Genus |
Mesocricetus
|
| Species |
auratus
|
| Age at sampling |
embryo
|
| Tissue |
embryo/fetus, whole
|
| Classification |
semi-normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_3164
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, MPI, PepB
|
| Chromosome mode |
|
37-94(50) : /44(6),45(2),46(7),47(21),48(2),58(1)/
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
9
|
| User's Publication |
0
|
| Reference |
|
211
Suzuki K, Miyoshi J, Watanabe M.
Function-dependent cooperation between oncogene activation and nonrandom chromosome change during tumorigenic conversion of Syrian hamster cells
Cancer Genet Cytogenet
1994
75(1):51-9
PubMed ID: 8039164
DOI: 10.1016/0165-4608(94)90215-1
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210
Sasai H, Higashi T, Nakamori S, Miyoshi J, Suzuki F, Nomura T, Kakunaga T.
Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours: detection of transforming genes in X-ray-related mouse tumours
Br J Cancer
1993
67(2):262-7
PubMed ID: 8431357
DOI: 10.1038/bjc.1993.50
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205
Suzuki K, Watanabe M, Miyoshi J.
Differences in effects of oncogenes on resistance of gamma rays, ultraviolet light, and heat shock
Radiat Res
1992
129(2):157-62
PubMed ID: 1734445
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208
Ohuchi T, Kurita Y, Sasai H, Miyoshi J, Nomura T, Toyoshima K.
Oncogenic activation of murine mos protein kinase by DNA rearrangement of its N-terminal coding region
Oncogene
1992
7(2):331-8
PubMed ID: 1532243
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203
Miyoshi J, Higashi T, Mukai H, Ohuchi T, Kakunaga T.
Structure and transforming potential of the human cot oncogene encoding a putative protein kinase
Mol Cell Biol
1991
11(8):4088-96
PubMed ID: 2072910
DOI: 10.1128/mcb.11.8.4088-4096.1991
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209
Aoki M, Akiyama T, Miyoshi J, Toyoshima K.
Identification and characterization of protein products of the cot oncogene with serine kinase activity
Oncogene
1991
6(9):1515-9
PubMed ID: 1833717
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212
Orita S, Higashi T, Kawasaki Y, Harada A, Igarashi H, Monden T, Morimoto H, Shimano T, Mori T, Miyoshi J.
A novel point mutation at codon 146 of the K-ras gene in a human colorectal cancer identified by the polymerase chain reaction
Virus Genes
1991
5(1):75-9
PubMed ID: 2017878
DOI: 10.1007/BF00571733
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204
Higashi T, Sasai H, Suzuki F, Miyoshi J, Ohuchi T, Takai S, Mori T, Kakunaga T.
Hamster cell line suitable for transfection assay of transforming genes
Proc Natl Acad Sci U S A
1990
87(7):2409-13
PubMed ID: 2181436
DOI: 10.1073/pnas.87.7.2409
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213
Nakamori S, Mukai H, Higashi T, Sasai H, Miyoshi J, Takai S, Mori T, Kakunaga T.
A simple and useful method for simultaneous screening of elevated levels of expression of a variety of oncogenes in malignant cells
Jpn J Cancer Res
1988
79(12):1311-7.
PubMed ID: 3148601
DOI: 10.1111/j.1349-7006.1988.tb01561.x
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