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Cell No. : Cell Name
RCB0453 : SHOK  update : 2022/03/15
CommentContact-inhibited hamster embryonal cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Jun
Originator Azuma, T. & Suzuki, F.
Year of deposit 1989
Another name GHE-c8
Animal hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3164
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Chromosome mode 37-94(50) : /44(6),45(2),46(7),47(21),48(2),58(1)/
Images
deposit info
lot info
Reference information Reference 0
User's Publication 0


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