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Cell No. : Cell Name
RCB0452 : HTC/C3  update : 2018/07/06
CommentSecretes IL-1,TGFα,KGM-CSF but not thyroglobulin
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Thyroid Group Mori Sugawa Clinic

606 Kikkoya-machi, Nakagyou-ku, Kyoto 604-8105 Japan
Dr.Sugawa, Hideo
Fax. +81-75-255-2002
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sugawa, Hideo
Originator Sugawa, H. & Inoue, D.
Year of deposit 1989
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 44 years
Tissue thyroid
Case history thryroid carcinoma
Metastastatic ability Yes
Classification cancer
Year of origin 1986
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 60-243(50) : /62(2),63(3),64(2),66(1),69(3),102(3)/
Reference information Reference 1
User's Publication 3


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Reference
3683  Enomoto T, Sugawa H, Inoue D, Miyamoto M, Kosugi S, Takahashi T, Kitamura N, Yamamoto I, Konishi J, Mori T, et al.  Establishment of a human undifferentiated thyroid cancer cell line producing several growth factors and cytokines.  Cancer  1990  65(9):1971-9  PubMed ID: 2196988

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User's Publication
11303  Cetti E, Di Marco T, Mauro G, Mazzoni M, Lecis D, Minna E, Gioiosa L, Brich S, Pagliardini S, Borrello MG, Pruneri G, Anania MC, Greco A.  Mitosis perturbation by MASTL depletion impairs the viability of thyroid tumor cells.  Cancer Lett.  2019    PubMed ID: 30445205
7264  Varinelli L, Caccia D, Volpi CC, Caccia C, De Bortoli M, Taverna E, Gualeni AV, Leoni V, Gloghini A, Manenti G, Bongarzone I.  4-IPP, a selective MIF inhibitor, causes mitotic catastrophe in thyroid carcinomas.  Endocr. Relat. Cancer  2015  22:759-75  PubMed ID: 26206776
1668  Yoshimoto K, Iwahana H, Fukuda A, Sano T, Saito S, Itakura M  Role of p53 mutations in endocrine tumorigenesis: mutation detection by polymerase chain reaction-single strand conformation polymorphism.  Cancer Res  1992  52:5061-4  PubMed ID: 1516062



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