Cell No. : Cell Name
RCB0451 : Anr13-1
update : 2022/03/15
|
Comment | EJ-ras oncogene transformed ARLJ301-3 |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
Basic information
|
Depositor |
Furukawa, Kazunori
|
Originator |
Sato, S.
|
Year of deposit |
1989
|
Original cell |
ARLJ301-3
|
Animal |
_rat
< Mammals
|
Strain name |
F344/DuCrj
|
Gender |
Male
|
Age at sampling |
10 weeks
|
Tissue |
liver
|
Classification |
transformed
|
Recombinant |
recombinant
|
Exogene |
EJ-ras
|
Year of origin |
1983
|
|
Lifespan |
infinite
|
Morphology |
other
|
Cellosaurus(Expasy) |
CVCL_4575
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
William's E + 10% FBS (heat inactivated)
|
Antibiotics |
|
Free
|
|
Passage method |
|
pipetting
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma/Acholeplasma |
|
(-)
|
Animal PCR |
|
OK
|
Isozyme |
|
LD, NP
|
Reference information |
Reference |
0
|
User's Publication |
2
|
User's Publication |
17949
Kageyama Y, Yamazaki Y, Afify AS, Ogawa Y, Okada T, Okuno H.
Stereoselective hydrolysis of xenobiotic esters by different cell lines from rat liver and hepatoma and its application to chiral prodrugs for designated growth suppression of cancer cells
Chirality
1995
7(4):297-304
PubMed ID: 7640174
DOI: 10.1002/chir.530070418
|
1635
Afify AS, Yamazaki Y, Kageyama Y, Yusa S, Ogawa Y, Okada T, Okuno H.
Different expression of esterase variants in rat hepatic and hepatoma-derived cell lines detected by electrophoresis
Z Naturforsch C J Biosci
1995
;50(9-10):664-8
PubMed ID: 8579684
DOI: 10.1515/znc-1995-9-1011
|