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Cell No. : Cell Name
RCB0449 : Anr4  update : 2022/03/15
CommentEJ-ras oncogene transformed ARLJ301-3
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Furukawa, Kazunori
Originator Sato, S.
Year of deposit 1989
Original cell ARLJ301-3
Animal _rat < Mammals
Strain name F344/DuCrj
Gender Male
Age at sampling 10 weeks
Tissue liver
Classification transformed
Recombinant recombinant
Exogene EJ-ras
Year of origin 1989
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_4105
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium William's E + 10% FBS (heat inactivated)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 0
User's Publication 4

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User's Publication
17949  Kageyama Y, Yamazaki Y, Afify AS, Ogawa Y, Okada T, Okuno H.  Stereoselective hydrolysis of xenobiotic esters by different cell lines from rat liver and hepatoma and its application to chiral prodrugs for designated growth suppression of cancer cells  Chirality  1995  7(4):297-304  PubMed ID: 7640174   DOI: 10.1002/chir.530070418
17956  Yamazaki Y, Ogawa Y, Afify AS, Kageyama Y, Okada T, Okuno H, Yoshii Y, Nose T.  Difference between cancer cells and the corresponding normal tissue in view of stereoselective hydrolysis of synthetic esters  Biochim Biophys Acta  1995  1243(3):300-8  PubMed ID: 7727503   DOI: 10.1016/0304-4165(94)00153-o
1635  Afify AS, Yamazaki Y, Kageyama Y, Yusa S, Ogawa Y, Okada T, Okuno H.  Different expression of esterase variants in rat hepatic and hepatoma-derived cell lines detected by electrophoresis  Z Naturforsch C J Biosci  1995  ;50(9-10):664-8  PubMed ID: 8579684   DOI: 10.1515/znc-1995-9-1011
17216  Yoshikatsu Ogawa, Yoshimitsu Yamazaki, Hiroaki Okuno  Stereoselectivity in the hydrolysis of synthetic esters by cultured cancer cells and normal tissue extracts of rat  Bioorganic & Medicinal Chemistry Letters  1994  4:757-760    DOI: 10.1016/s0960-894x(01)80196-7

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