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Cell No. : Cell Name
RCB0447 : ARLJ301-3  update : 2022/03/15
CommentDiploid rat liver epithelial cell line
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Furukawa, Kazunori
Originator Furukawa, Kazunori
Year of deposit 1989
Animal rat < Mammals
Strain name F344/DuCrj
Gender Male
Age at sampling 10 weeks
Tissue liver
Classification semi-normal
Year of origin 1983
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4107
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives William's E + 10% FBS (heat inactivated)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 39-84(50) : 39(1),41(7),42(36),81(2),83(1),84(3)
Reference information Reference 3
User's Publication 1

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1191  Furukawa, K., Yokoi, T., Kodama, T. & Mochizuki, Y.  Properties of a Newly Established Adult Rat Liver Epithlial Cell Strain and its Application to Transformation Assay to Detect Epigenetic Carcinogens.  Tumor Research  1987  22:87-102 
1193  Furukawa K, Shimada T, England P, Mochizuki Y, Williams, G M  Enrichment and characterization of clonogenic epithelial cells from adult rat liver and initiation of epithelial cell strains.  In Vitro Cell Dev Biol  1987  23:339-48  PubMed ID: 3294781
1192  Shimada T, Furukawa K, Kreiser, D M, Cawein A, Williams, G M  Induction of transformation by six classes of chemical carcinogens in adult rat liver epithelial cells.  Cancer Res  1983  43:5087-92  PubMed ID: 6616447

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User's Publication
2365  Takase-Yoden S, Watanabe R  Contribution of virus-receptor interaction to distinct viral proliferation of neuropathogenic and nonneuropathogenic murine leukemia viruses in rat glial cells.  J Virol  1999  73:4461-4  PubMed ID: 10196347

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