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細胞材料の再培養方法 ( HPS以外・ HPS )
細胞番号 : 細胞名
RCB0441 : SCCKN  update : 2024/08/14
細胞特性(Comment:英)Highly sensitive to bleomycin
細胞特性(日)舌癌。ブレオマイシン高感受性株である。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Urade, Masahiro
樹立者 Urade, M. & Mima, T.
寄託日 1989
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Male
齢 75 years
採取組織 tongue
病名 squamous carcinoma
転移 Yes
転移巣 subcutaneous tissue
細胞分類 cancer
初代培養日 1987
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_5362
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ/アコレプラズマ (-)
ウィルス（HIV) (-)
アイソザイム検査 LD, NP
染色体検査 51-136(50) : /66(4),67(2),68(6),69(6),70(8),71(6)/
個体識別検査 OK
文献情報 Reference(英) 7件
Reference(日) 3件
利用者成果(英) 26件
利用者成果(日) 0件

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Reference(英)
896  Yamamura M, Noguchi K, Nakano Y, Segawa E, Zushi Y, Takaoka K, Kishimoto H, Hashimoto-Tamaoki T, Urade M.  [Corrigendum] Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells  Int J Oncol  2016  49(5):2188  PubMed ID: 27666023   DOI: 10.3892/ijo.2016.3702
895  Yamamura M, Noguchi K, Nakano Y, Segawa E, Zushi Y, Takaoka K, Kishimoto H, Hashimoto-Tamaoki T, Urade M.  Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells.  Int J Oncol  2013  42(3):873-80  PubMed ID: 23292068   DOI: 10.3892/ijo.2013.1761.
894  Hiromoto T, Noguchi K, Yamamura M, Zushi Y, Segawa E, Takaoka K, Moridera K, Kishimoto H, Urade M.  Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma: relation to cell differentiation  Oncol Rep  2011  26(6):1415-21  PubMed ID: 21874256   DOI: 10.3892/or.2011.1429
196  Takahashi Y, Urade M, Kishimoto H, Arimoto T, Yanagisawa T, Yoshioka W.  Combined effect of bleomycin and SDB-ethylenediamine, a synthetic isoprenoid, on oral squamous carcinoma cell lines and transplantable nude mouse tumors  Gan To Kagaku Ryoho  1995  ;22(7):883-7  PubMed ID: 7540824  
195  Urade M, Ogura T, Uematsu T, Takahashi Y, Kishimoto H, Yoshioka W.  Induction of bleomycin resistance in a human oral squamous carcinoma cell line and characterisation of bleomycin-resistant and -sensitive clones  Eur J Cancer B Oral Oncol  1994  30B(6):409-14  PubMed ID: 7536509   DOI: 10.1016/0964-1955(94)90021-3
1465  Urade M, Ogura T, Mima T, Matsuya T  Establishment of human squamous carcinoma cell lines highly and minimally sensitive to bleomycin and analysis of factors involved in the sensitivity.  Cancer  1992  69:2589-97  PubMed ID: 1373668   DOI: 10.1002/1097-0142(19920515)69:10<2589::aid-cncr2820691032>3.0.co;2-y
1464  Urade, M., Ogura, T., Mima, T., Matsuya, T.  Acquisition of bleomycin (BLM) resistance in human squamous cell carcinoma transplantable in nude mice  The Cancer Journal  1990  3:192-196   

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Reference(日)
1146  浦出雅裕，小倉孝文，美馬孝至，松矢篤三  新たに分離された口腔偏平上皮癌細胞のブレオマイシン（ＢＬＭ）と多種抗癌剤に対する感受性    1989  393   
1149  Mima, M. et al  全身皮下に多発性転移をきたした舌偏平上皮癌の１例  日本口腔外科学会雑誌  1988  34:1976-1981    DOI: 10.5794/jjoms.34.1976
1148  Urade, M. et al  全身皮下に多発性転移を来たした舌癌の１症例と転移巣からの腫瘍細胞分離ＢＬＭ耐性獲得  頭頸部腫瘍  1987  14:380   

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利用者成果(英)
19730  Katase N, Kudo K, Ogawa K, Sakamoto Y, Nishimatsu SI, Yamauchi A, Fujita S.  DKK3/CKAP4 axis is associated with advanced stage and poorer prognosis in oral cancer  Oral Dis  2023  29(8):3193-3204  PubMed ID: 35708905   DOI: 10.1111/odi.14277
20057  Kitano H, Masaoka Y, Mamiya A, Fujiwara Y, Miki T, Hidai C.  Combination Cancer Therapy of a Del1 Fragment and Cisplatin Enhanced Therapeutic Efficiency In Vivo.  In Vivo  2021  35(2):779-791  PubMed ID: 33622870   DOI: 10.21873/invivo.12318
16975  Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K.  Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling  Anticancer Res  2021  41(4):1821-1830  PubMed ID: 33813387   DOI: 10.21873/anticanres.14948
6954  Hayashi H, Chiba T, Mihara-Tomiyama N, Negishi T, Kodama Y, Sakashita H, Imai K.  Domain structures of mucosa-associated lymphoid tissue lymphoma translocation 1 protein for nuclear localization in oral carcinoma cells and the proliferation inhibition.  Biochem Biophys Res Commun  2020  522(3):799-804  PubMed ID: 31791579   DOI: 10.1016/j.bbrc.2019.11.171
14346  Kitano H, Mamiya A, Ishikawa T, Fujiwara Y, Masaoka Y, Miki T, Hidai C.  An Epidermal Growth Factor Motif of Developmental Endothelial Locus 1 Protein Inhibits Efficient Angiogenesis in Explanted Squamous Cell Carcinoma In Vivo  Rev Invest Clin  2020  73(1):039-051.  PubMed ID: 33052897   DOI: 10.24875/RIC.20000375
11241  Oka T, Mizuno H, Sakata M, Fujita H, Yoshino T, Yamano Y, Utsumi K, Masujima T, Utsunomiya A.  Metabolic abnormalities in adult T-cell leukemia/lymphoma and induction of specific leukemic cell death using photodynamic therapy.  Sci Rep  2018    PubMed ID: 30297858   DOI: 10.1038/s41598-018-33175-7
10192  Hiroaki Yazama, Kazuyuki Kitatani, Kazunori Fujiwara, Misaki Kato, Mayumi Hashimoto-Nishimura, Katsuyuki Kawamoto, Kensaku Hasegawa, Hiroya Kitano, Alicja Bielawska, Jacek Bielawski, Toshiro Okazaki  Dietary Glucosylceramides Suppress Tumor Growth in a Mouse Xenograft Model of Head and Neck Squamous Cell Carcinoma by the Inhibition of Angiogenesis Through an Increase in Ceramide  Int J Clin Oncol  2015  20(3):438-46  PubMed ID: 25080062   DOI: 10.1007/s10147-014-0734-y
6078  Hotta H, Hamamura K, Yamashita K, Shibuya H, Tokuda N, Hashimoto N, Furukawa K, Yamamoto N, Hattori H, Toyokuni S, Ueda M, Furukawa K.  Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.  Glycoconj J  2013  30(6):585-97  PubMed ID: 23242548   DOI: 10.1007/s10719-012-9458-2
7808  Maemoto S, Yumoto M, Ibata M, Torizuka S, Ozawa N, Tatsumi S, Hashido M, Morikawa M, Maeda G, Imai K.  Mutational analysis of HRAS and KRAS genes in oral carcinoma cell lines.  Odontology  2012  100:149-55  PubMed ID: 21607592   DOI: 10.1007/s10266-011-0032-3
15740  Hashimoto T, Soeno Y, Maeda G, Taya Y, Aoba T, Nasu M, Kawashiri S, Imai K.  Progression of oral squamous cell carcinoma accompanied with reduced E-cadherin expression but not cadherin switch  PLoS One  2012  7(10):e4789  PubMed ID: 23110125   DOI: 10.1371/journal.pone.0047899
19127  Yoichi Akita, Ken-ichi Kozaki, Tomohiro Takeo, Motonobu Ohmura, Atsuko Nakagawa, Takeshi Yanagishita, Yoshiaki Kazaoka, Tadashige Nozaki, Kazuhisa Yokoo, Mitsuko Shinohara, Daisuke Watanabe, Tatsushi Kawai, Shiro Yamada, Yasuhiko Tamada, Kiyoshi Ohura, Yoshinari Matsumoto  Enhancement of the photodynamic effects on human oral squamous cell carcinoma cell lines by treatment with calcipotriol  Journal of Dermatological Science Supplement  2006  2:S57-S64    DOI: 10.1016/j.descs.2006.08.005
6147  Yoichi Akita  Enhancement of the photodynamic effects onhuman oral squamous cell carcinoma cell lines bytreatment with calcipotriol  Journal of Dermatological Science Supplement  2006  2:S57—S64   
3335  Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y.  Essential role for gene profiling analysis in the authentication of human cell lines.  Hum Cell  2006  19(1):43-8  PubMed ID: 16643607   DOI: 10.1111/j.1749-0774.2005.00007.x
16073  Hideki Watanabe, Yoshihiro Yamashita, Shuji Takada, Young Lim Choi, Shin-ichiro Fujiwara, Manabu Soda, Munehiro Enomoto, Kentaro Kurashina, Hisashi Hatanaka, Mikio Kusama, Hiroyuki Mano  Identification of Activated RRAS2 in Tongue Carcinoma Cell Line  Oral Medicine & Pathology  2006  11:121-126    DOI: 10.3353/omp.11.121
3428  Okamura, Hirohiko, Yoshida, Kaya, Morimoto, Hiroyuki, Haneji, Tatsuji  PTEN expression elicited by EGR-1 transcription factor in calyculin A-induced apoptotic cells.  J Cell Biochem  2005  94:117-25  PubMed ID: 15517593   DOI: 10.1002/jcb.20283
3108  Shibata, Masami, Kodani, Isamu, Osaki, Mitsuhiko, Araki, Kunio, Adachi, Hironobu, Ryoke, Kazuo, Ito, Hisao  Cyclo-oxygenase-1 and -2 expression in human oral mucosa, dysplasias and squamous cell carcinomas and their pathological significance.  Oral Oncol  2005  41:304-12  PubMed ID: 15743693   DOI: 10.1016/j.oraloncology.2004.09.009
3110  Kuratare I. et al.  Cell death induction of thymidine kinase gene transfer followed by ganciclovir treatment in oral squamous cell carcinoma cell lines.  Yonago Acta Med.  2005  48:63-74   
2906  Nishimaki, Haruaki, Kasai, Kenji, Kozaki, Ken ichi, Takeo, Tomohiro, Ikeda, Hiroshi, Saga, Shinsuke, Nitta, Masakazu, Itoh, Gen  A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.  Biochem Biophys Res Commun  2004  314:313-20  PubMed ID: 14733907   DOI: 10.1016/j.bbrc.2003.12.097
2883  Miyazawa, Jun, Mitoro, Akira, Kawashiri, Shuichi, Chada, Kiran K, Imai, Kazushi  Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.  Cancer Res  2004  64:2024-9  PubMed ID: 15026339   DOI: 10.1158/0008-5472.can-03-1855
19128  Uraguchi M, Morikawa M, Shirakawa M, Sanada K, Imai K.  Activation of WNT family expression and signaling in squamous cell carcinomas of the oral cavity  J Dent Res  2004  83(4):327-32  PubMed ID: 15044508   DOI: 10.1177/154405910408300411
17342  Okamura H, Yoshida K, Sasaki E, Morimoto H, Haneji T.  Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells  Int J Oncol  2004  25(4):1031-7  PubMed ID: 15375553  
19031  Nishimine M, Nakamura M, Mishima K, Kishi M, Kirita T, Sugimura M, Konishi N.  Id proteins are overexpressed in human oral squamous cell carcinomas  J Oral Pathol Med  2003  32(6):350-7  PubMed ID: 12787042   DOI: 10.1034/j.1600-0714.2003.00078.x
6642  Sasaki E, Okamoto Y, Yoshida K, Okamura H, Shimizu K, Nasu F, Morimoto H, Haneji T.  Transblot and cytochemical identification of avidin-interacting proteins in mitochondria of cultured cells.  Histochem Cell Biol  2003  120(4):327-33  PubMed ID: 14574588   DOI: 10.1007/s00418-003-0572-x
18744  Okamura H, Morimoto H, Fujita M, Nasu F, Sasakia E, Haneji T.  Suppression of Egr-1 expression in human oral squamous carcinoma cells by okadaic acid  Oral Oncol  2002  38(8):779-84  PubMed ID: 12570057   DOI: 10.1016/s1368-8375(02)00039-8
15593  Gunduz M, Ouchida M, Fukushima K, Ito S, Jitsumori Y, Nakashima T, Nagai N, Nishizaki K, Shimizu K.  Allelic loss and reduced expression of the ING3, a candidate tumor suppressor gene at 7q31, in human head and neck cancers  Oncogene  2002  21(28):4462-70  PubMed ID: 12080476   DOI: 10.1038/sj.onc.1205540
18003  Shrestha P, Sumitomo S, Lee CH, Nagahara K, Kamegai A, Yamanaka T, Takeuchi H, Kusakabe M, Mori M.  Tenascin: growth and adhesion modulation--extracellular matrix degrading function: an in vitro study  Eur J Cancer B Oral Oncol  1996  32B(2):106-13  PubMed ID: 8736172   DOI: 10.1016/0964-1955(95)00074-7

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