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Cell No. : Cell Name
RCB0441 : SCCKN  update : 2022/03/15
CommentHighly sensitive to bleomycin
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Urade, Masahiro
Originator Urade, M. & Mima, T.
Year of deposit 1989
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 75 years
Tissue tongue
Disease name squamous carcinoma
Metastastatic ability Yes
Metastatsd tissue subcutaneous tissue
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5362
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 51-136(50) : /66(4),67(2),68(6),69(6),70(8),71(6)/
STR(human) OK
Reference information Reference 2
User's Publication 18


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Reference
1465  Urade M, Ogura T, Mima T, Matsuya T  Establishment of human squamous carcinoma cell lines highly and minimally sensitive to bleomycin and analysis of factors involved in the sensitivity.  Cancer  1992  69:2589-97  PubMed ID: 1373668  
1464  Urade, M., Ogura, T., Mima, T., Matsuya, T.  Acquisition of bleomycin (BLM) resistance in human squamous cell carcinoma transplantable in nude mice  The Cancer Journal  1990  3:192-196   

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User's Publication
20057  Kitano H, Masaoka Y, Mamiya A, Fujiwara Y, Miki T, Hidai C.  Combination Cancer Therapy of a Del1 Fragment and Cisplatin Enhanced Therapeutic Efficiency In Vivo.  In Vivo  2021  35(2):779-791  PubMed ID: 33622870   DOI: 10.21873/invivo.12318
16975  Hotta H, Hamamura K, Shibuya H, Ohmi Y, Furukawa K, Furukawa K.  Lewis y Expressed in Oral Squamous Cell Carcinoma Attenuates Malignant Properties via Down-regulation of EGF Signaling  Anticancer Res  2021  41(4):1821-1830  PubMed ID: 33813387   DOI: 10.21873/anticanres.14948
6954  Hayashi H, Chiba T, Mihara-Tomiyama N, Negishi T, Kodama Y, Sakashita H, Imai K.  Domain structures of mucosa-associated lymphoid tissue lymphoma translocation 1 protein for nuclear localization in oral carcinoma cells and the proliferation inhibition.  Biochem Biophys Res Commun  2020  522(3):799-804  PubMed ID: 31791579   DOI: 10.1016/j.bbrc.2019.11.171
14346  Kitano H, Mamiya A, Ishikawa T, Fujiwara Y, Masaoka Y, Miki T, Hidai C.  An Epidermal Growth Factor Motif of Developmental Endothelial Locus 1 Protein Inhibits Efficient Angiogenesis in Explanted Squamous Cell Carcinoma In Vivo  Rev Invest Clin  2020  73(1):039-051.  PubMed ID: 33052897   DOI: 10.24875/RIC.20000375
11241  Oka T, Mizuno H, Sakata M, Fujita H, Yoshino T, Yamano Y, Utsumi K, Masujima T, Utsunomiya A.  Metabolic abnormalities in adult T-cell leukemia/lymphoma and induction of specific leukemic cell death using photodynamic therapy.  Sci Rep  2018    PubMed ID: 30297858   DOI: 10.1038/s41598-018-33175-7
10192  Hiroaki Yazama, Kazuyuki Kitatani, Kazunori Fujiwara, Misaki Kato, Mayumi Hashimoto-Nishimura, Katsuyuki Kawamoto, Kensaku Hasegawa, Hiroya Kitano, Alicja Bielawska, Jacek Bielawski, Toshiro Okazaki  Dietary Glucosylceramides Suppress Tumor Growth in a Mouse Xenograft Model of Head and Neck Squamous Cell Carcinoma by the Inhibition of Angiogenesis Through an Increase in Ceramide  Int J Clin Oncol  2015  20(3):438-46  PubMed ID: 25080062   DOI: 10.1007/s10147-014-0734-y
6078  Hotta H, Hamamura K, Yamashita K, Shibuya H, Tokuda N, Hashimoto N, Furukawa K, Yamamoto N, Hattori H, Toyokuni S, Ueda M, Furukawa K.  Lewis y antigen is expressed in oral squamous cell carcinoma cell lines and tissues, but disappears in the invasive regions leading to the enhanced malignant properties irrespective of sialyl-Lewis x.  Glycoconj J  2013  30(6):585-97  PubMed ID: 23242548   DOI: 10.1007/s10719-012-9458-2
15740  Hashimoto T, Soeno Y, Maeda G, Taya Y, Aoba T, Nasu M, Kawashiri S, Imai K.  Progression of oral squamous cell carcinoma accompanied with reduced E-cadherin expression but not cadherin switch  PLoS One  2012  7(10):e4789  PubMed ID: 23110125   DOI: 10.1371/journal.pone.0047899
7808  Maemoto S, Yumoto M, Ibata M, Torizuka S, Ozawa N, Tatsumi S, Hashido M, Morikawa M, Maeda G, Imai K.  Mutational analysis of HRAS and KRAS genes in oral carcinoma cell lines.  Odontology  2011  100:149-55  PubMed ID: 21607592   DOI: 10.1007/s10266-011-0032-3
6147  Yoichi Akita  Enhancement of the photodynamic effects onhuman oral squamous cell carcinoma cell lines bytreatment with calcipotriol  Journal of Dermatological Science Supplement  2006  2:S57—S64   
3335  Yoshino K, Iimura E, Saijo K, Iwase S, Fukami K, Ohno T, Obata Y, Nakamura Y.  Essential role for gene profiling analysis in the authentication of human cell lines.  Hum Cell  2006  19(1):43-8  PubMed ID: 16643607   DOI: 10.1111/j.1749-0774.2005.00007.x
16073  Hideki Watanabe, Yoshihiro Yamashita, Shuji Takada, Young Lim Choi, Shin-ichiro Fujiwara, Manabu Soda, Munehiro Enomoto, Kentaro Kurashina, Hisashi Hatanaka, Mikio Kusama, Hiroyuki Mano  Identification of Activated RRAS2 in Tongue Carcinoma Cell Line  Oral Medicine & Pathology  2006  11:121-126    DOI: 10.3353/omp.11.121
3428  Okamura, Hirohiko, Yoshida, Kaya, Morimoto, Hiroyuki, Haneji, Tatsuji  PTEN expression elicited by EGR-1 transcription factor in calyculin A-induced apoptotic cells.  J Cell Biochem  2005  94:117-25  PubMed ID: 15517593   DOI: 10.1002/jcb.20283
3108  Shibata, Masami, Kodani, Isamu, Osaki, Mitsuhiko, Araki, Kunio, Adachi, Hironobu, Ryoke, Kazuo, Ito, Hisao  Cyclo-oxygenase-1 and -2 expression in human oral mucosa, dysplasias and squamous cell carcinomas and their pathological significance.  Oral Oncol  2005  41:304-12  PubMed ID: 15743693   DOI: 10.1016/j.oraloncology.2004.09.009
3110  Kuratare I. et al.  Cell death induction of thymidine kinase gene transfer followed by ganciclovir treatment in oral squamous cell carcinoma cell lines.  Yonago Acta Med.  2005  48:63-74   
2906  Nishimaki, Haruaki, Kasai, Kenji, Kozaki, Ken ichi, Takeo, Tomohiro, Ikeda, Hiroshi, Saga, Shinsuke, Nitta, Masakazu, Itoh, Gen  A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.  Biochem Biophys Res Commun  2004  314:313-20  PubMed ID: 14733907   DOI: 10.1016/j.bbrc.2003.12.097
2883  Miyazawa, Jun, Mitoro, Akira, Kawashiri, Shuichi, Chada, Kiran K, Imai, Kazushi  Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.  Cancer Res  2004  64:2024-9  PubMed ID: 15026339   DOI: 10.1158/0008-5472.can-03-1855
15593  Gunduz M, Ouchida M, Fukushima K, Ito S, Jitsumori Y, Nakashima T, Nagai N, Nishizaki K, Shimizu K.  Allelic loss and reduced expression of the ING3, a candidate tumor suppressor gene at 7q31, in human head and neck cancers  Oncogene  2002  21(28):4462-70  PubMed ID: 12080476   DOI: 10.1038/sj.onc.1205540



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