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Cell No. : Cell Name
RCB0440 : LC-2/ad  update : 2020/09/24
CommentAdenocarcinoma, moderately differentiated. Cell growth is slow. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Itoh, Hiroshi
Originator Kataoka, Hiroaki
Year of deposit 1989
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 51 years
Tissue lung, pleural fluid
Case history adenocarcinoma
Classification cancer
Year of origin 1986
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives (HamF12 : RPMI1640 = 1 : 1) + 25mM HEPES + 15% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2-3 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 99-206(50) : /101(4),102(2),103(11),104(14),105(7)/
Images
deposit info
lot info
Reference information Reference 1
User's Publication 22


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Reference
1780  Kataoka H, Itoh H, Seguchi K, Koono M  Establishment and characterization of a human lung adenocarcinoma cell line (LC-2/ad) producing α1-anititrypsin In Vitro  Acta Pathologica Japonica  1993  43:566-573  PubMed ID: 8291444

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User's Publication
12021  Wanna-Udom S, Terashima M, Lyu H, Ishimura A, Takino T, Sakari M, Tsukahara T, Suzuki T.  The m6A methyltransferase METTL3 contributes to Transforming Growth Factor-beta-induced epithelial-mesenchymal transition of lung cancer cells through the regulation of JUNB.  Biochem. Biophys. Res. Commun.  2020    PubMed ID: 31982139
12063  Kuchitsu Y, Nagashio R, Igawa S, Kusuhara S, Tsuchiya B, Ichinoe M, Satoh Y, Naoki K, Murakumo Y, Saegusa M, Sato Y.  TRAP1 is a predictive biomarker of platinum-based adjuvant chemotherapy benefits in patients with resected lung adenocarcinoma.  Biomed. Res.  2020    PubMed ID: 32092740
11833  Hiyoshi Y, Sato Y, Ichinoe M, Nagashio R, Hagiuda D, Kobayashi M, Kusuhara S, Igawa S, Shiomi K, Goshima N, Murakumo Y, Saegusa M, Satoh Y, Masuda N, Naoki K.  Prognostic significance of IMMT expression in surgically-resected lung adenocarcinoma.  Thorac Cancer  2019    PubMed ID: 31583841
11040  Koh Furugaki, Marie Mochizuki, Mirei Kohno, Sei Shu, Naoki Harada, Yasushi Yoshimura   Expression of C-terminal ALK, RET, or ROS1 in lung cancer cells with or without fusion  BMC Cancer  2019  19(1):301  PubMed ID: 30943926
11223  Terashima M, Ishimura A, Wanna-Udom S, Suzuki T.  MEG8 long noncoding RNA contributes to epigenetic progression of the epithelial-mesenchymal transition of lung and pancreatic cancer cells.  J. Biol. Chem.  2018    PubMed ID: 30262664
4183  Ohara K, Ohkuri T, Kumai T, Nagato T, Nozaki Y, Ishibashi K, Kosaka A, Nagata M, Harabuchi S, Ohara M, Oikawa K, Aoki N, Harabuchi Y, Celis E, Kobayashi H.  Targeting phosphorylated p53 to elicit tumor-reactive T helper responses against head and neck squamous cell carcinoma.  Oncoimmunology  2018    PubMed ID: 30510853
9949  Chang H, Sung JH, Moon SU, Kim HS, Kim JW, Lee JS.  EGF Induced RET Inhibitor Resistance in CCDC6-RET Lung Cancer Cells.  Yonsei Med. J.  2017  58:9-18  PubMed ID: 27873490
9950  Terashima M, Tange S, Ishimura A, Suzuki T.  MEG3 Long Noncoding RNA Contributes to the Epigenetic Regulation of Epithelial-Mesenchymal Transition in Lung Cancer Cell Lines.  J. Biol. Chem.  2017  292:82-99  PubMed ID: 27852821
10280  Seike M, Kim CH, Zou F, Noro R, Chiba M, Ishikawa A, Κunugi S, Kubota K, Gemma A.  AXL and GAS6 co-expression in lung adenocarcinoma as a prognostic classifier.  Oncol. Rep.  2017  37:3261-3269  PubMed ID: 28440492
10408  Terashima M, Ishimura A, Wanna-Udom S, Suzuki T.  Epigenetic regulation of epithelial-mesenchymal transition by KDM6A histone demethylase in lung cancer cells.  Biochem. Biophys. Res. Commun.  2017  490:1407-1413  PubMed ID: 28698146
10464  Reeser JW, Martin D, Miya J, Kautto EA, Lyon E, Zhu E, Wing MR, Smith A, Reeder M, Samorodnitsky E, Parks H, Naik KR, Gozgit J, Nowacki N, Davies KD, Varella-Garcia M, Yu L, Freud AG, Coleman J, Aisner DL, Roychowdhury S.  Validation of a Targeted RNA Sequencing Assay for Kinase Fusion Detection in Solid Tumors.  J Mol Diagn  2017  19:682-696  PubMed ID: 28802831
10922    Loss of IL-24 expression facilitates invasion in lung adenocarcinoma   Int J Clin Exp Pathol  2017  10:173-185 
9522  Nishijima N, Seike M, Soeno C, Chiba M, Miyanaga A, Noro R, Sugano T, Matsumoto M, Kubota K, Gemma A.  miR-200/ZEB axis regulates sensitivity to nintedanib in non-small cell lung cancer cells.  Int. J. Oncol.  2016  48:937-44  PubMed ID: 26783187
7452  Liu G, Wu Q, Liu G, Song X, Zhang J.  Psoriasin (S100A7) is a novel biomarker for lung squamous cell carcinoma in humans.  Cancer Cell Int.  2015  15:18  PubMed ID: 25745359
4866  Kodama T, Tsukaguchi T, Satoh Y, Yoshida M, Watanabe Y, Kondoh O, Sakamoto H.  Alectinib shows potent antitumor activity against RET-rearranged non-small cell lung cancer.  Mol Cancer Ther  2014  13(12):2910-8  PubMed ID: 25349307
7714  Huqun, Ishikawa R, Zhang J, Miyazawa H, Goto Y, Shimizu Y, Hagiwara K, Koyama N.  Enhancer of zeste homolog 2 is a novel prognostic biomarker in nonsmall cell lung cancer.  Cancer  2011  118:1599-606  PubMed ID: 21837672
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
2481  Okudela K, Yazawa T, Woo T, Sakaeda M, Ishii J, Mitsui H, Shimoyamada H, Sato H, Tajiri M, Ogawa N, Masuda M, Takahashi T, Sugimura H, Kitamura H.  Down-regulation of DUSP6 expression in lung cancer: its mechanism and potential role in carcinogenesis.  Am J Pathol  2009  175(2):867-81  PubMed ID: 19608870
3580  Ito T, Maki N, Hazeki O, Sasaki K, Nekooki M.  Extracellular and transmembrane region of a podocalyxin-like protein 1 fragment identified from colon cancer cell lines.  Cell Biol Int  2007  31(12):1518-24  PubMed ID: 17719804
3061  Nagai, Yoshiaki, Miyazawa, Hitoshi, Tanaka, Tomoaki, Udagawa, Kiyoshi, Kato, Motoyasu, Fukuyama, Shunichiro, Yokote, Akemi, Kobayashi, Kunihiko, Kanazawa, Minoru, Hagiwara, Koichi, Hagiwara K  Genetic heterogeneity of the epidermal growth factor receptor in non-small cell lung cancer cell lines revealed by a rapid and sensitive detection system, the peptide nucleic acid-locked nucleic acid PCR clamp.  Cancer Res  2005  65:7276-82  PubMed ID: 16105816
3338  Seike, Masahiro, Kondo, Tadashi, Fujii, Kazuyasu, Yamada, Tesshi, Gemma, Akihiko, Kudoh, Shoji, Hirohashi, Setsuo  Proteomic signature of human cancer cells.  Proteomics  2004  4:2776-88  PubMed ID: 15352251
2483  Niki T, Iba S, Tokunou M, Yamada T, Matsuno Y, Hirohashi S  Expression of vascular endothelial growth factors A, B, C, and D and their relationships to lymph node status in lung adenocarcinoma.  Clin Cancer Res  2000  6:2431-9  PubMed ID: 10873096



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