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Cell No. : Cell Name
RCB0439 : LC-1F  update : 2024/01/17
CommentHuman cell line derived from lung cancer. Squamous cell carcinoma. Floating cells (non-adherent cells). LC-1/sq-SF (RCB0438), LC-1F (RCB0439), and LC-1/sq (RCB0455) are derived from the same patient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Itoh, Hiroshi
Originator Kataoka, Hiroaki
Year of deposit 1989
Original cell LC-1/sq
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 69 years
Tissue lung
Classification cancer
Year of origin 1986
History H. Kataoka
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1372
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium (HamF12 : RPMI1640 = 1 : 1) + 25mM HEPES + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 73-181(50) : /83(5),84(1),85(11),86(5),87(3),88(2)/
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 9

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1779  Itoh H, Kataoka H, Koita H, Nabeshima K, Inoue T, Kangawa K, Koono M  Establishment of a new human cancer cell line secreting protease nexin-II/amyloid beta protein precursor derived from squamous-cell carcinoma of lung.  Int J Cancer  1991  49:436-43  PubMed ID: 1917142   DOI: 10.1002/ijc.2910490322

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User's Publication
13332  Hase H, Aoki M, Matsumoto K, Nakai S, Nagata T, Takeda A, Ueda K, Minami K, Kitae K, Jingushi K, Ueda Y, Yamamoto M, Furukawa T, Sato M, Tsujikawa K.  Cancer type‑SLCO1B3 promotes epithelial‑mesenchymal transition resulting in the tumour progression of non‑small cell lung cancer.  Oncol Rep  2021    PubMed ID: 33155667   DOI: 10.3892/or.2020.7839
13773  Saigusa D, Motoike IN, Saito S, Zorzi M, Aoki Y, Kitamura H, Suzuki M, Katsuoka F, Ishii H, Kinoshita K, Motohashi H, Yamamoto M.  Impacts of NRF2 activation in non-small-cell lung cancer cell lines on extracellular metabolites  Cancer Sci  2020  111(2):667-678  PubMed ID: 31828882   DOI: 10.1111/cas.14278
19029  Kanamori-Katayama M, Kaiho A, Ishizu Y, Okamura-Oho Y, Hino O, Abe M, Kishimoto T, Sekihara H, Nakamura Y, Suzuki H, Forrest AR, Hayashizaki Y.  LRRN4 and UPK3B are markers of primary mesothelial cells  PLoS One  2011  6(10):e25391  PubMed ID: 21984916   DOI: 10.1371/journal.pone.0025391
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
18072  Cheng CM, Tsuneyama K, Matsui K, Takahashi H, Ishizawa S, Takano Y.  Cytoplasmic expression of c-erbB2 in non-small cell lung cancers  Virchows Arch  2005  446(6):596-603  PubMed ID: 15902485   DOI: 10.1007/s00428-005-1258-7
16489  Seike M, Kondo T, Fujii K, Okano T, Yamada T, Matsuno Y, Gemma A, Kudoh S, Hirohashi S.  Proteomic signatures for histological types of lung cancer  Proteomics  2005  5(11):2939-48  PubMed ID: 15996008   DOI: 10.1002/pmic.200401166
3338  Seike, Masahiro, Kondo, Tadashi, Fujii, Kazuyasu, Yamada, Tesshi, Gemma, Akihiko, Kudoh, Shoji, Hirohashi, Setsuo  Proteomic signature of human cancer cells.  Proteomics  2004  4:2776-88  PubMed ID: 15352251   DOI: 10.1002/pmic.200300795
16491  Nakanishi H, Suda T, Katoh M, Watanabe A, Igishi T, Kodani M, Matsumoto S, Nakamoto M, Shigeoka Y, Okabe T, Oshimura M, Shimizu E.  Loss of imprinting of PEG1/MEST in lung cancer cell lines  Oncol Rep  2004  12(6):1273-8  PubMed ID: 15547750  
3134  Yoshimasu, T. et al  Integrins α2β1,α5β1 and αvβ5 are related to tumor growth and metastasis of non-small cell cancer   Japanese Journal of Lung Cancer  2001  41:111-115    DOI: 10.2482/haigan.41.111

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