Cell No. : Cell Name RCB0438 : LC-1/sq-SF  update : 2022/10/13
Comment			Human cell line derived from lung cancer. Squamous cell carcinoma. A subline of LC-1/sq (RCB0455) able to be cultured in serum-free conditions. LC-1/sq-SF (RCB0438), LC-1F (RCB0439), and LC-1/sq (RCB0455) are derived from the same patient. Application consideration
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Itoh, Hiroshi
		Originator	Kataoka, Hiroaki
		Year of deposit	1989
		Original cell	LC-1/sq
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	69 years
		Tissue	lung
		Classification	cancer
		Year of origin	1986
		History	H. Kataoka
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_5480
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent/Suspension cells
		Culture medium		(HamF12 : RPMI1640 = 1 : 1) + 25mM HEPES + 5x10 -10M T3 + 10 -9M Retinoic acid + 5μg/ml Insulin + 5μg/ml Transferrin + 5ng/ml Selenium
		Antibiotics		Free
		Passage method		dilution, pipetting
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : once/2 weeks, Medium Renewal : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium : 2 x FM1 = 1 : 1
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Virus (HIV)		(-)
		Isozyme		LD, NP, G6PD
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

Top

Reference

1779  Itoh H, Kataoka H, Koita H, Nabeshima K, Inoue T, Kangawa K, Koono M  Establishment of a new human cancer cell line secreting protease nexin-II/amyloid beta protein precursor derived from squamous-cell carcinoma of lung.  Int J Cancer  1991  49:436-43  PubMed ID: 1917142   DOI: 10.1002/ijc.2910490322

Top

User's Publication

14789  Chang A, Liu L, Ashby JM, Wu D, Chen Y, O'Neill SS, Huang S, Wang J, Wang G, Cheng D, Tan X, Petty WJ, Pasche BC, Xiang R, Zhang W, Sun P.  Recruitment of KMT2C/MLL3 to DNA Damage Sites Mediates DNA Damage Responses and Regulates PARP Inhibitor Sensitivity in Cancer  Cancer Res  2021  81(12):3358-3373  PubMed ID: 33853832   DOI: 10.1158/0008-5472.CAN-21-0688

6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x