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Cell No. : Cell Name
RCB0438 : LC-1/sq-SF  update : 2022/10/13
CommentHuman cell line derived from lung cancer. Squamous cell carcinoma. A subline of LC-1/sq (RCB0455) able to be cultured in serum-free conditions. LC-1/sq-SF (RCB0438), LC-1F (RCB0439), and LC-1/sq (RCB0455) are derived from the same patient. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Itoh, Hiroshi
Originator Kataoka, Hiroaki
Year of deposit 1989
Original cell LC-1/sq
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 69 years
Tissue lung
Classification cancer
Year of origin 1986
History H. Kataoka
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_5480
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium (HamF12 : RPMI1640 = 1 : 1) + 25mM HEPES + 5x10 -10M T3 + 10 -9M Retinoic acid + 5μg/ml Insulin + 5μg/ml Transferrin + 5ng/ml Selenium
Antibiotics Free
Passage method dilution, pipetting
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/2 weeks, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Isozyme LD, NP, G6PD
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 2

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1779  Itoh H, Kataoka H, Koita H, Nabeshima K, Inoue T, Kangawa K, Koono M  Establishment of a new human cancer cell line secreting protease nexin-II/amyloid beta protein precursor derived from squamous-cell carcinoma of lung.  Int J Cancer  1991  49:436-43  PubMed ID: 1917142   DOI: 10.1002/ijc.2910490322

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User's Publication
14789  Chang A, Liu L, Ashby JM, Wu D, Chen Y, O'Neill SS, Huang S, Wang J, Wang G, Cheng D, Tan X, Petty WJ, Pasche BC, Xiang R, Zhang W, Sun P.  Recruitment of KMT2C/MLL3 to DNA Damage Sites Mediates DNA Damage Responses and Regulates PARP Inhibitor Sensitivity in Cancer  Cancer Res  2021  81(12):3358-3373  PubMed ID: 33853832   DOI: 10.1158/0008-5472.CAN-21-0688
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x

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