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Cell No. : Cell Name
RCB0437 : HUC-Fm2  update : 2022/03/15
CommentNormal umbilical cord fibroblast, male
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishida, M.& Ohno, T.
Originator Ohno, Tadao
Year of deposit 1989
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue umbilical cord
Classification normal
Year of origin 1989
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4683
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Achoreplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-92(50) : 44(2),45(8),46(32),47(4),92(4)
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
893  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

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