Cell No. : Cell Name
RCB0436 : HUC-F2
update : 2024/09/04
|
| Comment | Normal umbilical cord fibroblast, female |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
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Basic information
|
Depositor |
Nishida, M.& Ohno, T.
|
| Originator |
Ohno, Tadao
|
| Year of deposit |
1989
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Tissue |
umbilical cord
|
| Classification |
normal
|
| Year of origin |
1989
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_4682
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM ALPHA + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
(-)
|
| Chromosome mode |
|
44-72(50) : 44(4),45(9),46(36),72(1)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
11
|
| User's Publication |
|
17446
Shimizu Y, Miyakura R, Otsuka Y.
Nuclear receptor subfamily 4, group A, member 1 inhibits extrinsic apoptosis and reduces caspase-8 activity in H2O2-induced human HUC-F2 fibroblasts
Redox Rep
2015
20(2):81-8
PubMed ID: 25330024
DOI: 10.1179/1351000214Y.0000000109
|
|
16595
Harada G, Neng Q, Fujiki T, Katakura Y.
Molecular mechanisms for the p38-induced cellular senescence in normal human fibroblast
J Biochem
2014
156(5):283-90
PubMed ID: 24920674
DOI: 10.1093/jb/mvu040
|
|
7604
Yamashita S, Ogawa K, Ikei T, Udono M, Fujiki T, Katakura Y.
SIRT1 prevents replicative senescence of normal human umbilical cord fibroblast through potentiating the transcription of human telomerase reverse transcriptase gene.
Biochem. Biophys. Res. Commun.
2012
417:630-4
PubMed ID: 22197555
DOI: 10.1016/j.bbrc.2011.12.021
|
|
7706
Atsumi Y, Fujimori H, Fukuda H, Inase A, Shinohe K, Yoshioka Y, Shikanai M, Ichijima Y, Unno J, Mizutani S, Tsuchiya N, Hippo Y, Nakagama H, Masutani M, Teraoka H, Yoshioka K.
Onset of quiescence following p53 mediated down-regulation of H2AX in normal cells.
PLoS ONE
2011
6:e23432
PubMed ID: 21858116
DOI: 10.1371/journal.pone.0023432
|
|
9109
Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.
Establishment of induced pluripotent stem cells from human neonatal tissues.
Hum Cell
2010
23(3):113-8
PubMed ID: 20973836
DOI: 10.1111/j.1749-0774.2010.00091.x
|
|
5396
Hosomi O, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamakura F, Kudo S.
Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.
Glycoconj J
2009
26(2):189-98
PubMed ID: 18726690
DOI: 10.1007/s10719-008-9175-z
|
|
8788
Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.
Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.
Cell Biol. Int.
2008
32:1-7
PubMed ID: 17890111
DOI: 10.1016/j.cellbi.2007.08.001
|
|
18718
Ohshima S.
Abnormal mitosis in hypertetraploid cells causes aberrant nuclear morphology in association with H2O2-induced premature senescence
Cytometry A
2008
73(9):808-15
PubMed ID: 18618767
DOI: 10.1002/cyto.a.20604
|
|
2824
Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K
Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.
Gene Ther
2005
12:852-6
PubMed ID: 15750614
DOI: 10.1038/sj.gt.3302483
|
|
2723
Ohshima S
Apoptosis in stress-induced and spontaneously senescent human fibroblasts.
Biochem Biophys Res Commun
2004
324:241-6
PubMed ID: 15465009
DOI: 10.1016/j.bbrc.2004.09.044
|
|
5393
Harada M, Imai J, Okuno S, Suzuki T.
Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.
J Control Release
2000
69(3):389-97
PubMed ID: 11102679
DOI: 10.1016/s0168-3659(00)00320-5
|
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