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Cell No. : Cell Name
RCB0436 : HUC-F2  update : 2022/12/05
CommentNormal umbilical cord fibroblast, female
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nishida, M.& Ohno, T.
Originator Ohno, Tadao
Year of deposit 1989
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue umbilical cord
Classification normal
Year of origin 1989
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4682
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) (-)
Chromosome mode 44-72(50) : 44(4),45(9),46(36),72(1)
STR(human) OK
Reference information Reference 1
User's Publication 10


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Reference
893  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504

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User's Publication
17446  Shimizu Y, Miyakura R, Otsuka Y.  Nuclear receptor subfamily 4, group A, member 1 inhibits extrinsic apoptosis and reduces caspase-8 activity in H2O2-induced human HUC-F2 fibroblasts  Redox Rep  2015  20(2):81-8  PubMed ID: 25330024   DOI: 10.1179/1351000214Y.0000000109
16595  Harada G, Neng Q, Fujiki T, Katakura Y.  Molecular mechanisms for the p38-induced cellular senescence in normal human fibroblast  J Biochem  2014  156(5):283-90  PubMed ID: 24920674   DOI: 10.1093/jb/mvu040
7604  Yamashita S, Ogawa K, Ikei T, Udono M, Fujiki T, Katakura Y.  SIRT1 prevents replicative senescence of normal human umbilical cord fibroblast through potentiating the transcription of human telomerase reverse transcriptase gene.  Biochem. Biophys. Res. Commun.  2012  417:630-4  PubMed ID: 22197555   DOI: 10.1016/j.bbrc.2011.12.021
7706  Atsumi Y, Fujimori H, Fukuda H, Inase A, Shinohe K, Yoshioka Y, Shikanai M, Ichijima Y, Unno J, Mizutani S, Tsuchiya N, Hippo Y, Nakagama H, Masutani M, Teraoka H, Yoshioka K.  Onset of quiescence following p53 mediated down-regulation of H2AX in normal cells.  PLoS ONE  2011  6:e23432  PubMed ID: 21858116   DOI: 10.1371/journal.pone.0023432
9109  Fujioka T, Shimizu N, Yoshino K, Miyoshi H, Nakamura Y.  Establishment of induced pluripotent stem cells from human neonatal tissues.  Hum Cell  2010  23(3):113-8  PubMed ID: 20973836   DOI: 10.1111/j.1749-0774.2010.00091.x
5396  Hosomi O, Misawa Y, Takeya A, Matahira Y, Sugahara K, Kubohara Y, Yamakura F, Kudo S.  Novel oligosaccharide has suppressive activity against human leukemia cell proliferation.  Glycoconj J  2009  26(2):189-98  PubMed ID: 18726690   DOI: 10.1007/s10719-008-9175-z
8788  Hiroyama T, Sudo K, Aoki N, Miharada K, Danjo I, Fujioka T, Nagasawa T, Nakamura Y.  Human umbilical cord-derived cells can often serve as feeder cells to maintain primate embryonic stem cells in a state capable of producing hematopoietic cells.  Cell Biol. Int.  2008  32:1-7  PubMed ID: 17890111   DOI: 10.1016/j.cellbi.2007.08.001
2824  Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K  Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.  Gene Ther  2005  12:852-6  PubMed ID: 15750614   DOI: 10.1038/sj.gt.3302483
2723  Ohshima S  Apoptosis in stress-induced and spontaneously senescent human fibroblasts.  Biochem Biophys Res Commun  2004  324:241-6  PubMed ID: 15465009   DOI: 10.1016/j.bbrc.2004.09.044
5393  Harada M, Imai J, Okuno S, Suzuki T.  Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.  J Control Release  2000  69(3):389-97  PubMed ID: 11102679   DOI: 10.1016/s0168-3659(00)00320-5



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