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Cell No. : Cell Name
RCB0434 : J774.1  update : 2021/06/24
CommentMacrophage-like cell
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sakagami, Hiroshi
Originator Peter, Ralph
Year of deposit 1989
Another name J774A.1
Animal _mouse < Mammals
Strain name BALB/c
Gender Female
Tissue ascites
Classification other
History Nagumo, Noboru (Showa Univ.)
Lifespan infinite
Morphology other (macrophage-like)
Cellosaurus(Expasy) CVCL_4770
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 7
User's Publication 45

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1185  Sakagami H, Ikeda M, Konno K  Stimulation of tumor necrosis factor-induced human myelogenous leukemic cell differentiation by high molecular weight PSK subfraction.  Biochem Biophys Res Commun  1989  162:597-603  PubMed ID: 2757633
1189  Sakagami H, Ikeda M, Unten S, Takeda K, Murayama J, Hamada A, Kimura K, Komatsu N, Konno K  Antitumor activity of polysaccharide fractions from pine cone extract of Pinus parviflora Sieb. et Zucc.  Anticancer Res  1987  7:1153-9  PubMed ID: 3481681
1294  Ralph P, Nakoinz I  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture.  Cancer Res  1977  37:546-50  PubMed ID: 318922
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
1176  Ralph P, Prichard J, Cohn M  Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity.  J Immunol  1975  114:898-905  PubMed ID: 1089721
1177  Ralph P, Nakoinz I  Phagocytosis and cytolysis by a macrophage tumour and its cloned cell line.  Nature  1975  257:393-4  PubMed ID: 1101071

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User's Publication
13905  Tobita K, Hoshi F, Ohki T, Watanabe I.  Protein denature extracts of Lactobacillus crispatus KT-11 strain promote interleukin 12p40 production via Toll-like receptor 2 in J774.1 cell culture  J Food Biochem  2021  45(2):e13599.  PubMed ID: 33368417
20183  Tamai R, Mashima I, Kiyoura Y.  Alendronate Augments Lipid A-Induced IL-1α Release via Activation of ASC but Not Caspase-11.  Inflammation  2021    PubMed ID: 34080091
11977  Tamai R, Suzuki K, Mashima I, Kiyoura Y.  MPMBP down-regulates Toll-like receptor (TLR) 2 ligand-induced proinflammatory cytokine production by inhibiting NF-κB but not AP-1 activation.  Int. Immunopharmacol.  2020    PubMed ID: 31901621
12981  Watanabe S, Usui-Kawanishi F, Karasawa T, Kimura H, Kamata R, Komada T, Inoue Y, Mise N, Kasahara T, Takahashi M.  Glucose regulates hypoxia-induced NLRP3 inflammasome activation in macrophages.  J Cell Physiol  2020    PubMed ID: 32115713
13022  Bracamonte ME, Álvarez AM, Sosa AM, Hoyos CL, Lauthier JJ, Cajal SP, Juarez M, Uncos RE, Sánchez-Valdéz FJ, Acuña L, Diosque P, Basombrío MA, Nasser JR, Hashiguchi Y, Korenaga M, Barroso PA, Marco JD.  High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens.  PLoS One  2020    PubMed ID: 32379842
13348  Noritake K, Aki T, Isa S, Uemura K.  Pyroptotic cell death by exposure to 1-butanol in H9c2 cardiomyoblastoma cells.  Heliyon  2020    PubMed ID: 33294662
13734  Matsugaki A, Matsumoto S, Nakano T.  A Novel Role of Interleukin-6 as a Regulatory Factor of Inflammation-Associated Deterioration in Osteoblast Arrangement  Int J Mol Sci  2020  21(18):6659  PubMed ID: 32932973
20124  Tan XW, Kobayashi K, Shen L, Inagaki J, Ide M, Hwang SS, Matsuura E.  Antioxidative attributes of rice bran extracts in ameliorative effects of atherosclerosis-associated risk factors.  Heliyon  2020  6(12):e05743  PubMed ID: 33376820
14498  Hirano S, Kanno S.  Relevance of autophagy markers to cytotoxicity of zinc compounds in macrophages  Toxicol In Vitro  2020  65:104816  PubMed ID: 32126253
12304  Tomo Shimizu, Osamu Miyazaki, Takeo Iwamoto, Tomoyuki Usui, Ryo Sato, Chika Hiraishi, Hiroshi Yoshida  A new method for measuring cholesterol efflux capacity uses stable isotope-labeled, not radioactive-labeled, cholesterol  J Lipid Res  2019  60(11):1959-1967  PubMed ID: 31455616
10747  Tamai R, Kiyoura Y.  Alendronate augments lipid A-induced IL-1β release and Smad3/NLRP3/ASC-dependent cell death.  Life Sci.  2018  198:8-17  PubMed ID: 29438662
10832  Kitamura H, Saito N, Fujimoto J, Nakashima KI, Fujikura D.  Brazilian propolis ethanol extract and its component kaempferol induce myeloid-derived suppressor cells from macrophages of mice in vivo and in vitro.  BMC Complement Altern Med  2018  18:138  PubMed ID: 29720160
11239  Yano H, Uchida M, Saito T, Aoki T, Kremenik MJ, Oyanagi E.  Reduction of Real-Time Imaging of M1 Macrophage Chemotaxis toward Damaged Muscle Cells is PI3K-Dependent.  Antioxidants (Basel)  2018    PubMed ID: 30297636
9855  Konda N, Saeki N, Nishino S, Ogawa K.  Truncated EphA2 likely potentiates cell adhesion via integrins as well as infiltration and/or lodgment of a monocyte/macrophage cell line in the red pulp and marginal zone of the mouse spleen, where ephrin-A1 is prominently expressed in the vasculature.  Histochem. Cell Biol.  2017  147:317-339  PubMed ID: 27665280
10402  Zhang R, Inagawa H, Takahashi M, Kawanishi H, Kazumura K, Tsuchiya H, Morishita N, Kobayashi Y, Masaki T, Kobara H, Soma GI.  Measurement of the Phagocytic Activity of Human Peripheral Blood Using a Highly Sensitive Fluorometric Detection Device Without Hemolysis.  Anticancer Res.  2017  37:3897-3903  PubMed ID: 28668892
10425  Sangkanjanavanich N, Kawai M, Kakuda T, Takai S.  Rescue of an intracellular avirulent Rhodococcus equi replication defect by the extracellular addition of virulence-associated protein A.  J. Vet. Med. Sci.  2017  79:1323-1326  PubMed ID: 28690290
10508  Kitamura H, Ishino T, Shimamoto Y, Okabe J, Miyamoto T, Takahashi E, Miyoshi I.  Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells.  Mediators Inflamm.  2017  2017:6909415  PubMed ID: 29138532
14248  Kobayashi M, Usui-Kawanishi F, Karasawa T, Kimura H, Watanabe S, Mise N, Kayama F, Kasahara T, Hasebe N, Takahashi M.  The cardiac glycoside ouabain activates NLRP3 inflammasomes and promotes cardiac inflammation and dysfunction  PLoS One  2017  12(5):e0176676  PubMed ID: 28493895
7312  Motoyama K, Sako A, Ibrahim Abu Hashim I, Higashi T, Arima H.  Effects of dendrimer/cyclodextrin conjugates as gene transfer carriers on nitric oxide production from macrophages.  J. Pharm. Pharmacol.  2016  68:598-607  PubMed ID: 26076676
9562  Hotta A, Fujita O, Uda A, Yamamoto Y, Sharma N, Tanabayashi K, Yamada A, Morikawa S.  Virulence of representative Japanese Francisella tularensis and immunologic consequences of infection in mice.  Microbiol. Immunol.  2016  60:168-76  PubMed ID: 26853540
9711  Ando T, Komatsu T, Naiki Y, Takahashi K, Yokochi T, Watanabe D, Koide N.  GSK2656157, a PERK inhibitor, reduced LPS-induced IL-1β production through inhibiting Caspase 1 activation in macrophage-like J774.1 cells.  Immunopharmacol Immunotoxicol  2016  38:298-302  PubMed ID: 27251848
9734  Zhang R, Kobayashi Y, Kazumura K, Tsuchiya H, Morishita N, Inagawa H, Soma G.  Development of an Evaluation Device for Phagocytic Activity of New Phagocytes Using Simple and pH-sensitive Particles that Do Not Require Pre-treatment.  Anticancer Res.  2016  36:3613-8  PubMed ID: 27354631
9739  Hachiya R, Shiihashi T, Shirakawa I, Iwasaki Y, Matsumura Y, Oishi Y, Nakayama Y, Miyamoto Y, Manabe I, Ochi K, Tanaka M, Goda N, Sakai J, Suganami T, Ogawa Y.  The H3K9 methyltransferase Setdb1 regulates TLR4-mediated inflammatory responses in macrophages.  Sci Rep  2016  6:28845  PubMed ID: 27349785
10875    Immune cell response and subsequent bone formation induced by implantation of octacalcium phosphate in a rat tibia defect  RSC Adv  2016  6, 57475-57484 
7231  Yoshida K, Maekawa T, Zhu Y, Renard-Guillet C, Chatton B, Inoue K, Uchiyama T, Ishibashi K, Yamada T, Ohno N, Shirahige K, Okada-Hatakeyama M, Ishii S.  The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory.  Nat. Immunol.  2015  16:1034-43  PubMed ID: 26322480
7281  Kitahara N, Morisaka H, Aoki W, Takeda Y, Shibasaki S, Kuroda K, Ueda M.  Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.  AMB Express  2015  5:127  PubMed ID: 26179440
7503  Ito M, Matsuoka I.  Inhibition of P2Y6 receptor-mediated phospholipase C activation and Ca(2+) signalling by prostaglandin E2 in J774 murine macrophages.  Eur. J. Pharmacol.  2015  749:124-32  PubMed ID: 25614334
9257  Yoshimitsu Okazaki, Emiko Gotoh  Metal Ion Effects on Different Types of Cell Line, Metal Ion Incorporation Into L929 and MC3T3-E1 Cells, and Activation of Macrophage-Like J774.1 Cells  Mater Sci Eng C Mater Biol Appl  2013  33(4):1993-2001  PubMed ID: 23498224
8085  Sakagami H, Zhou L, Kawano M, Thet MM, Tanaka S, Machino M, Amano S, Kuroshita R, Watanabe S, Chu Q, Wang QT, Kanamoto T, Terakubo S, Nakashima H, Sekine K, Shirataki Y, Zhang CH, Uesawa Y, Mohri K, Kitajima M, Oizumi H, Oizumi T.  Multiple biological complex of alkaline extract of the leaves of Sasa senanensis Rehder.  In Vivo  2010  24:735-43  PubMed ID: 20952742
8207  Kaori Yoshino, Kaoru Saijo, Chikako Noro and Yukio Nakamura  Development of a simple method to determine the mouse strain from which cultured cell lines originated  Interdisciplinary Bio Central   2010  2:0014 
8813  Kanno S, Furuyama A, Hirano S.  A murine scavenger receptor MARCO recognizes polystyrene nanoparticles.  Toxicol. Sci.  2007  97:398-406  PubMed ID: 17361018
6295  Barroso PA, Marco JD, Calvopina M, Kato H, Korenaga M, Hashiguchi Y.  A trial of immunotherapy against Leishmania amazonensis infection in vitro and in vivo with Z-100, a polysaccharide obtained from Mycobacterium tuberculosis, alone or combined with meglumine antimoniate.  J Antimicrob Chemother  2007  59(6):1123-9  PubMed ID: 17439977
8866  Matsumoto M, Benno Y.  Anti-inflammatory metabolite production in the gut from the consumption of probiotic yogurt containing Bifidobacterium animalis subsp. lactis LKM512.  Biosci. Biotechnol. Biochem.  2006  70:1287-92  PubMed ID: 16794305
3345  Moro, Toru, Takatori, Yoshio, Ishihara, Kazuhiko, Konno, Tomohiro, Takigawa, Yorinobu, Matsushita, Tomiharu, Chung, Ung-Il, Nakamura, Kozo, Kawaguchi, Hiroshi  Surface grafting of artificial joints with a biocompatible polymer for preventing periprosthetic osteolysis.  Nat Mater  2004  3:829-36  PubMed ID: 15502835
3222  Liu, Qingping, Kobayashi, Kazuko, Furukawa, Jun-ichi, Inagaki, Junko, Sakairi, Nobuo, Iwado, Akimasa, Yasuda, Tatsuji, Koike, Takao, Voelker, Dennis R, Matsuura, Eiji  Omega-carboxyl variants of 7-ketocholesteryl esters are ligands for beta(2)-glycoprotein I and mediate antibody-dependent uptake of oxidized LDL by macrophages.  J Lipid Res  2002  43:1486-95  PubMed ID: 12235181
6451  Ippoushi K, Itou H, Azuma K, Higashio H.  Effect of naturally occurring organosulfur compounds on nitric oxide production in lipopolysaccharide-activated macrophages.  Life Sci  2002  71(4):411-9  PubMed ID: 12044841
12848  Morita H, He F, Fuse T, Ouwehand AC, Hashimoto H, Hosoda M, Mizumachi K, Kurisaki J.  Cytokine production by the murine macrophage cell line J774.1 after exposure to lactobacilli  Biosci Biotechnol Biochem  2002  66(9):1963-6  PubMed ID: 12400700
2473  Tanaka I, Ishihara H  Enhanced expression of the early retrotransposon in C3H mouse-derived myeloid leukemia cells.  Virology  2001  280:107-14  PubMed ID: 11162824
2956  Shibaki T, Suzutani T, Yoshida I, Ogasawara M, Azuma M  Participation of type I interferon in the decreased virulence of the UL13 gene-deleted mutant of herpes simplex virus type 1.  J Interferon Cytokine Res  2001  21:279-85  PubMed ID: 11429158
4871  Feng R, Kabayama A, Uchida K, Hoshino H, Miwa M.  Cell-free entry of human T-cell leukemia virus type 1 to mouse cells.  Jpn J Cancer Res  2001  92(4):410-6  PubMed ID: 11346463
6165  Kobayashi K, Matsuura E, Liu Q, Furukawa J, Kaihara K, Inagaki J, Atsumi T, Sakairi N, Yasuda T, Voelker DR, Koike T.  A specific ligand for beta(2)-glycoprotein I mediates autoantibody-dependent uptake of oxidized low density lipoprotein by macrophages.  J Lipid Res  2001  42(5):697-709  PubMed ID: 11352976
5393  Harada M, Imai J, Okuno S, Suzuki T.  Macrophage-mediated activation of camptothecin analogue T-2513-carboxymethyl dextran conjugate (T-0128): possible cellular mechanism for antitumor activity.  J Control Release  2000  69(3):389-97  PubMed ID: 11102679
2360  Tsutsui T, Kawaguchi H, Fujino A, Sakai A, Kaji H, Nakamura T  Exposure of macrophage-like cells to titanium particles does not affect bone resorption, but inhibits bone formation.  J Orthop Sci  1999  4:32-8  PubMed ID: 9914427
5325  Shige H, Ishikawa T, Suzukawa M, Nishiwaki M, Yamashita T, Nakajima K, Ito T, Higashi K, Ayaori M, Yonemura A, Nestel P, Nakamura H.  Vitamin E reduces cholesterol esterification and uptake of acetylated low density lipoprotein in macrophages.  Lipids  1998  33(12):1169-75  PubMed ID: 9930402
3024  Harada Y, Nagao S, Nakamura M, Okada F, Tanigawa Y  Effect of lipopolysaccharide on thymidine salvage as related to macrophage activation.  Immunology  1995  84:247-53  PubMed ID: 7751001

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