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Cell No. : Cell Name
RCB0432 : MT-5  update : 2022/03/15
CommentMNNG-initiated clone of BALB/c 3T3 clone A31
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hiwasa, Takaki
Originator Hiwasa, Takaki
Year of deposit 1989
Another name M14T5
Original cell BALB/c 3T3 clone A31
Animal _mouse < Mammals
Strain name BALB/c
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U900
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% NBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
1140  Hiwasa T, Tanigawara C, Sakiyama S  Defects of cyclic adenosine 3':5'-monophosphate-dependent protein kinases in initiated clones derived from BALB/c 3T3 mouse fibroblasts.  Cancer Res  1987  47:953-9  PubMed ID: 3026623  

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User's Publication
2379  Maeda T, Hiranuma H, Jikko A  Differential expression of the splicing regulatory factor genes during two-step chemical transformation in a BALB/3T3-derived cell line, MT-5.  Carcinogenesis  1999  20:2341-4  PubMed ID: 10590230   DOI: 10.1093/carcin/20.12.2341



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