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Cell No. : Cell Name
RCB0431 : T24  update : 2021/05/20
CommentBladder transitional-cell carcinoma
Comment from the depositor
Terms and conditionsResearch purpose only. Send APPROVAL Form(C-0006) directly to RCB.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.Cell Bank (ISHIKAWA, Hiroshi)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor RCB (Noda, Makoto)
Originator Bubenick, J.
Year of deposit 1989
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 81 years
Tissue bladder
Disease name transitional-cell carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0554
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives McCoy's 5A + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 79-90(50) : /81(3),82(10),83(8),84(5),85(13),86(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 9

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4680  Capon DJ, Chen EY, Levinson AD, Seeburg PH, Goeddel DV.  Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.  Nature  1983  302:33-7  PubMed ID: 6298635
1173  Bubeník J, Baresová M, Viklický V, Jakoubková J, Sainerová H, Donner J.  Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen.  Int J Cancer  1973  11(3):765-73  PubMed ID: 4133950
1172  Bubeník J, Perlmann P, Helmstein K, Moberger G.  Cellular and humoral immune responses to human urinary bladder carcinomas.  Int J Cancer  1970  5(3):310-9  PubMed ID: 5452065

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User's Publication
11877  Nozaki S, Nakatani Y, Mawatari A, Shibata N, Hume WE, Hayashinaka E, Wada Y, Doi H, Watanabe Y.  18F-FIMP: a LAT1-specific PET probe for discrimination between tumor tissue and inflammation.  Sci Rep  2019    PubMed ID: 31673030
14444  Kameyama K, Horie K, Mizutani K, Kato T, Fujita Y, Kawakami K, Kojima T, Miyazaki T, Deguchi T, Ito M.  Enzalutamide inhibits proliferation of gemcitabine-resistant bladder cancer cells with increased androgen receptor expression  Int J Oncol  2017  50(1):75-84  PubMed ID: 27909718
5877  Yamamoto H, Okumura K, Toshima S, Mukaisho K, Sugihara H, Hattori T, Kato M, Asano S.  FXYD3 protein involved in tumor cell proliferation is overproduced in human breast cancer tissues.  Biol Pharm Bull  2009  32(7):1148-54  PubMed ID: 19571376
13714  Futami K, Kumagai E, Makino H, Goto H, Takagi M, Shimamoto A, Furuichi Y.  Induction of mitotic cell death in cancer cells by small interference RNA suppressing the expression of RecQL1 helicase  Cancer Sci  2008  99(1):71-80  PubMed ID: 17953710
9046  Shin-ichi Kohno 1, Chenhong Luo, Fumi Goshima, Yukihiro Nishiyama, Tetsutaro Sata, Yoshinari Ono  Herpes Simplex Virus Type 1 Mutant HF10 Oncolytic Viral Therapy for Bladder Cancer  Urology  2005  66 (5), 1116-21  PubMed ID: 16286150
2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159
3759  Kataoka H, Uchino H, Iwamura T, Seiki M, Nabeshima K, Koono M.  Enhanced tumor growth and invasiveness in vivo by a carboxyl-terminal fragment of alpha1-proteinase inhibitor generated by matrix metalloproteinases: a possible modulatory role in natural killer cytotoxicity.  Am J Pathol  1999  154(2):457-68  PubMed ID: 10027404
5969  Morita M, Yoshiuchi N, Arakawa H, Nishimura S.  CMAP: a novel cystatin-like gene involved in liver metastasis.  Cancer Res  1999  59(1):151-8  PubMed ID: 9892200
2181  Ryusuke Futatsuya M.D., Ph.D. Hitomi Kimura M.D. Masatoshi Maeda Ph.D. Miki Shoji Ph.D. Takashi Honda Ph.D.  Assessment of intrinsic radiosensitivity of human bladder cancer cell lines using colorimetric‐MTT and micronucleus assays  Radiation Oncology Investigations  1996  4:249-254 

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