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Cell No. : Cell Name
RCB0431 : T24  update : 2023/06/06
CommentBladder transitional-cell carcinoma
Comment from the depositor
Terms and conditionsResearch purpose only. Send APPROVAL Form(C-0006) directly to RIKEN Cell Bank.
approver's address
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RIKEN, BioResource Research Center
Cell Engineering Division
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Noda, Makoto
Originator Bubenick, J.
Year of deposit 1989
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 81 years
Tissue bladder
Disease name transitional-cell carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0554
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium McCoy's 5A + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 79-90(50) : /81(3),82(10),83(8),84(5),85(13),86(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 6
User's Publication 15

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190  Bubeník J, Kieler J, Tromholt V, Steven K, Indrová M, Toulcová A.  Activation of killer cells from blood of urinary bladder carcinoma patients by short-term treatment with recombinant interleukin 2  Folia Biol (Praha)  1986  32(3):183-94  PubMed ID: 3488923  
189  Bubeńik J, Kieler J, Perlmann P, Paulie S, Koho H, Christensen B, Dienstbier Z, Koprivová H, Pospísil J, Poucková P.  Monoclonal antibodies against human urinary bladder carcinomas: selectivity and utilization for gamma scintigraphy  Eur J Cancer Clin Oncol  1985  21(6):701-10  PubMed ID: 3894033   DOI: 10.1016/0277-5379(85)90267-6
191  Bubeník J, Kieler J, Perlmann P, Paulie S, Koho H, Christensen B, Dienstbier Z, Koprivová H, Pospísil J, Novák F.  Tumour localization of radiolabelled monoclonal antibody in mice bearing human urinary bladder (T24) carcinoma xenografts  Folia Biol (Praha)  1985  31(2):176-84  PubMed ID: 3894060  
4680  Capon DJ, Chen EY, Levinson AD, Seeburg PH, Goeddel DV.  Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.  Nature  1983  302:33-7  PubMed ID: 6298635   DOI: 10.1038/302033a0
1173  Bubeník J, Baresová M, Viklický V, Jakoubková J, Sainerová H, Donner J.  Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen.  Int J Cancer  1973  11(3):765-73  PubMed ID: 4133950   DOI: 10.1002/ijc.2910110327
1172  Bubeník J, Perlmann P, Helmstein K, Moberger G.  Cellular and humoral immune responses to human urinary bladder carcinomas.  Int J Cancer  1970  5(3):310-9  PubMed ID: 5452065   DOI: 10.1002/ijc.2910050303

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User's Publication
16352  Shimizu R, Ohira T, Yagyu T, Yumioka T, Yamaguchi N, Iwamoto H, Morizane S, Hikita K, Honda M, Takenaka A, Kugoh H.  Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9  Oncol Lett  2022  23(3):92  PubMed ID: 35154423   DOI: 10.3892/ol.2022.13212
11877  Nozaki S, Nakatani Y, Mawatari A, Shibata N, Hume WE, Hayashinaka E, Wada Y, Doi H, Watanabe Y.  18F-FIMP: a LAT1-specific PET probe for discrimination between tumor tissue and inflammation.  Sci Rep  2019    PubMed ID: 31673030   DOI: 10.1038/s41598-019-52270-x
14444  Kameyama K, Horie K, Mizutani K, Kato T, Fujita Y, Kawakami K, Kojima T, Miyazaki T, Deguchi T, Ito M.  Enzalutamide inhibits proliferation of gemcitabine-resistant bladder cancer cells with increased androgen receptor expression  Int J Oncol  2017  50(1):75-84  PubMed ID: 27909718   DOI: 10.3892/ijo.2016.3781
5877  Yamamoto H, Okumura K, Toshima S, Mukaisho K, Sugihara H, Hattori T, Kato M, Asano S.  FXYD3 protein involved in tumor cell proliferation is overproduced in human breast cancer tissues.  Biol Pharm Bull  2009  32(7):1148-54  PubMed ID: 19571376   DOI: 10.1248/bpb.32.1148
13714  Futami K, Kumagai E, Makino H, Goto H, Takagi M, Shimamoto A, Furuichi Y.  Induction of mitotic cell death in cancer cells by small interference RNA suppressing the expression of RecQL1 helicase  Cancer Sci  2008  99(1):71-80  PubMed ID: 17953710   DOI: 10.1111/j.1349-7006.2007.00647.x
9046  Shin-ichi Kohno 1, Chenhong Luo, Fumi Goshima, Yukihiro Nishiyama, Tetsutaro Sata, Yoshinari Ono  Herpes Simplex Virus Type 1 Mutant HF10 Oncolytic Viral Therapy for Bladder Cancer  Urology  2005  66 (5), 1116-21  PubMed ID: 16286150   DOI: 10.1016/j.urology.2005.05.041
2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159   DOI: 10.1073/pnas.121616198
16905  Kawai K, Hayashi H, Ozaki Y, Saijo K, Liu SQ, Akaza H, Ohno T.  Assessment of the number of local cytotoxic T lymphocytes required for degradation of micrometer-size tumor spheroids  Cytotechnology  2001  37(1):31-40  PubMed ID: 19002912   DOI: 10.1023/A:1016139010531
18150  Kono T, Watanabe M, Koyama K, Kishimoto T, Fukushima S, Sugimura T, Wakabayashi K.  Cytotoxic activity of pierisin, from the cabbage butterfly, Pieris rapae, in various human cancer cell lines  Cancer Lett  1999  137(1):75-81  PubMed ID: 10376796   DOI: 10.1016/s0304-3835(98)00346-2
3759  Kataoka H, Uchino H, Iwamura T, Seiki M, Nabeshima K, Koono M.  Enhanced tumor growth and invasiveness in vivo by a carboxyl-terminal fragment of alpha1-proteinase inhibitor generated by matrix metalloproteinases: a possible modulatory role in natural killer cytotoxicity.  Am J Pathol  1999  154(2):457-68  PubMed ID: 10027404   DOI: 10.1016/s0002-9440(10)65292-3
5969  Morita M, Yoshiuchi N, Arakawa H, Nishimura S.  CMAP: a novel cystatin-like gene involved in liver metastasis.  Cancer Res  1999  59(1):151-8  PubMed ID: 9892200  
18022  Ryusuke Futatsuya, Hitomi Kimura, Masatoshi Maeda, Miki Shoji, Takashi Honda  Assessment of intrinsic radiosensitivity of human bladder cancer cell lines using colorimetric-MTT and micronucleus assays  Radiation Oncology Investigations  1996  4:249-254   
2181  Ryusuke Futatsuya M.D., Ph.D. Hitomi Kimura M.D. Masatoshi Maeda Ph.D. Miki Shoji Ph.D. Takashi Honda Ph.D.  Assessment of intrinsic radiosensitivity of human bladder cancer cell lines using colorimetric‐MTT and micronucleus assays  Radiation Oncology Investigations  1996  4:249-254    DOI: 10.1002/(SICI)1520-6823
17930  Nabeshima K, Kishi J, Kurogi T, Komada N, Kataoka H, Okada Y, Koono M.  Stimulation of TIMP-1 and metalloproteinase production in co-cultures of human tumor cells and human fibroblasts  Cancer Lett  1994  78(1-3):133-40  PubMed ID: 8180955   DOI: 10.1016/0304-3835(94)90042-6
1902  Shu Qin Liu, Kaoru Saijo, Makoto Kodama, Tadao Ohno  Intelligent matrices for tumor-specific cytotoxic T lymphocytes  Materials Science and Engineering: C  1994  2:61-65    DOI: 10.1016/0928-4931(94)90030-2

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