Cell No. : Cell Name
RCB0423 : BL312
update : 2024/12/23
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| Comment | BSL2. Bovine derived lymphocyte. It is described in the paper that this cell line produces bovine leukemia virus (BLV). Syncytium formation assay performed in RIKEN Cell Bank also suggested production of BLV (Figure). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Aida, Yoko
|
| Originator |
Itohara, Shigemi
|
| Year of deposit |
1989
|
| Another name |
B312
|
| Animal |
_bovine
< Mammals
|
| Strain name |
Holstein-Friesian cow
|
| Gender |
Unknown
|
| Age at sampling |
3 years
|
| Tissue |
peripheral blood lymphocytes
|
| Classification |
cancer
|
| History |
originator -> depositor
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_5117
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
RPMI1640 + 15% FBS + 50μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
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1 : 4 split
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| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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