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Cell No. : Cell Name
RCB0423 : BL312  update : 2022/11/07
CommentBovine derived lymphocyte. It is described in the paper that this cell line produces bovine leukemia virus (BLV). In relation to the production of virus, RIKEN Cell Bank has not confirmed it.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Aida, Yoko
Originator Itohara, Shigemi
Year of deposit 1989
Another name B312
Animal bovine < Mammals
Strain name Holstein-Friesian cow
Gender Unknown
Age at sampling 3 years
Tissue peripheral blood lymphocytes
Classification cancer
History originator -> depositor
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_5117
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 15% FBS + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 0

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1101  Itohara S, Sekikawa K, Mizuno Y, Kono Y, Nakajima H.  Establishment of bovine leukemia virus-producing and -nonproducing B-lymphoid cell lines and their proviral genomes.  Leuk Res  1987  11(5):407-14  PubMed ID: 3033403   DOI: 10.1016/0145-2126(87)90071-3

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