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Cell No. : Cell Name
RCB0420 : BHK-21C13-2P  update : 2022/03/14
CommentCulturable in suspension, cf. ECACC ( BHK-21C13-3P). Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 84111301 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA84111301 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Originator Radlett, P. & Draper, M.
Year of deposit 1989
Animal hamster < Mammals
Strain name Syrian
Genus Mesocricetus
Species auratus
Tissue kidney
Classification other
History overseas, ECACC
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8837
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives MEM (suspension) + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 1


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Reference
2078  Macpherson, I., A.  AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.  Virology  1964  23:291-294  PubMed ID: 14187925   DOI: 10.1016/0042-6822(64)90301-0
2077  Ian Macpherson  Characteristics of a Hamster Cell Clone Transformed by Polyoma Virus  J Natl Cancer Inst  1963  30:795-815    DOI: 10.1093/jnci/30.4.795
18  Macpherson,I. and Stoker,M.  Polyoma transformation of hamster cell clones----an investigation of genetic factors affecting cell competence.  Virology  1962  16:147-151  PubMed ID: 14468055  

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User's Publication
8312  Lee BH, Oyane A, Tsurushima H, Shimizu Y, Sasaki T, Koshizaki N.  A new approach for hydroxyapatite coating on polymeric materials using laser-induced precursor formation and subsequent aging.  ACS Appl Mater Interfaces  2009  1:1520-4  PubMed ID: 20355955   DOI: 10.1021/am900183e



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