Cell No. : Cell Name
RCB0418 : KU-17
update : 2024/10/02
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| Comment | BSL2. Bovine leukemia cell line. It is described in the paper that this cell line produces bovine leukosis virus (BLV). In relation to the production of virus, RIKEN Cell Bank has not confirmed it. Application consideration |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Aida, Yoko
|
| Originator |
Koyama, Hiroyuki
|
| Year of deposit |
1989
|
| Animal |
_bovine
< Mammals
|
| Gender |
Female
|
| Age at sampling |
8 years
|
| Tissue |
blood
|
| Disease name |
B cell leukemia
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_4713
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 4 split
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| SC frequency |
|
Subculture : 2 times/week
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
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| Isozyme |
|
LD, NP
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| Reference information |
Reference |
2
|
| User's Publication |
0
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| Reference |
|
17486
Yamanaka MP, Saito S, Hosomichi K, Aida Y.
Comprehensive Comparison of Novel Bovine Leukemia Virus (BLV) Integration Sites between B-Cell Lymphoma Lines BLSC-KU1 and BLSC-KU17 Using the Viral DNA Capture High-Throughput Sequencing Method
Viruses
2022
14(5):995
PubMed ID: 35632737
DOI: 10.3390/v14050995
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1790
Koyama H1, Hohdatsu T, Satake M, Kobayashi M, Ashizawa T, Sugimoto K, Yoshikawa H, Okada K, Yoshikawa T, Saito H.
Properties of nine continuous B-cell lines established from enzootic bovine leukosis tumors.
J Vet Med B
1992
39:32-38
PubMed ID: 1580106
DOI: 10.1111/j.1439-0450.1992.tb01134.x
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