Cell No. : Cell Name RCB0418 : KU-17  update : 2026/03/27
Comment			BSL2. Bovine leukemia cell line. It is described in the paper that this cell line produces bovine leukosis virus (BLV). In relation to the production of virus, RIKEN Cell Bank has not confirmed it. Application consideration
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Aida, Yoko
		Originator	Koyama, Hiroyuki
		Year of deposit	1989
		Animal	_bovine < Mammals
		Gender	Female
		Age at sampling	8 years
		Tissue	blood
		Disease name	B cell leukemia
		Classification	cancer
		Lifespan	infinite
		Morphology	lymphocyte-like
		BSL	BSL2
		SDS	SDS_DMSO(Japanese)
		Cellosaurus(Expasy)	CVCL_4713
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		DNA barcoding		OK
		Isozyme		LD, NP
Reference information		Reference	2
		User's Publication	0

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Reference

17486  Yamanaka MP, Saito S, Hosomichi K, Aida Y.  Comprehensive Comparison of Novel Bovine Leukemia Virus (BLV) Integration Sites between B-Cell Lymphoma Lines BLSC-KU1 and BLSC-KU17 Using the Viral DNA Capture High-Throughput Sequencing Method  Viruses  2022  14(5):995  PubMed ID: 35632737   DOI: 10.3390/v14050995

1790  Koyama H1, Hohdatsu T, Satake M, Kobayashi M, Ashizawa T, Sugimoto K, Yoshikawa H, Okada K, Yoshikawa T, Saito H.  Properties of nine continuous B-cell lines established from enzootic bovine leukosis tumors.  J Vet Med B  1992  39:32-38  PubMed ID: 1580106   DOI: 10.1111/j.1439-0450.1992.tb01134.x

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