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Cell No. : Cell Name
RCB0418 : KU-17  update : 2022/11/07
CommentBovine leukemia cell line. It is described in the paper that this cell line produces bovine leukosis virus (BLV). In relation to the production of virus, RIKEN Cell Bank has not confirmed it. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Aida, Yoko
Year of deposit 1989
Animal bovine < Mammals
Gender Female
Age at sampling 8 years
Tissue blood
Disease name B cell leukemia
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
BSL BSL2
Cellosaurus(Expasy) CVCL_4713
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
1790  Koyama H1, Hohdatsu T, Satake M, Kobayashi M, Ashizawa T, Sugimoto K, Yoshikawa H, Okada K, Yoshikawa T, Saito H.  Properties of nine continuous B-cell lines established from enzootic bovine leukosis tumors.  J Vet Med B  1992  39:32-38  PubMed ID: 1580106   DOI: 10.1111/j.1439-0450.1992.tb01134.x

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