Cell No. : Cell Name
RCB0417 : KU-1
update : 2024/01/17
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| Comment | Bovine B cell leukemia cell line. It is described in the paper that bovine leukaemia virus (BLV) particles and BLV antigen are both negative. In relation to the production of virus, RIKEN Cell Bank has not confirmed it. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Aida, Yoko
|
| Originator |
Koyama, Hiroyuki
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| Year of deposit |
1989
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| Animal |
_bovine
< Mammals
|
| Gender |
Female
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| Age at sampling |
4 years
|
| Tissue |
blood
|
| Classification |
cancer
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| Year of origin |
1982
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|
Lifespan |
infinite
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| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_4711
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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DMEM (low glucose) + 20% FBS
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| Antibiotics |
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Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 4 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
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| User's Publication |
1
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| Reference |
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17486
Yamanaka MP, Saito S, Hosomichi K, Aida Y.
Comprehensive Comparison of Novel Bovine Leukemia Virus (BLV) Integration Sites between B-Cell Lymphoma Lines BLSC-KU1 and BLSC-KU17 Using the Viral DNA Capture High-Throughput Sequencing Method
Viruses
2022
14(5):995
PubMed ID: 35632737
DOI: 10.3390/v14050995
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1789
Koyama H, Imamura Y, Yoshikawa H, Kajikawa O, Itohara S, Mizuno Y, Yoshikawa T, Saito H.
Establishment of a Continuous Cell Line Derived from Leukaemic Cattle
J Vet Med B
1986
33:586-596
PubMed ID: 3026121
DOI: 10.1111/j.1439-0450.1986.tb00073.x
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187
Onuma M, Koyama H, Aida Y, Okada K, Ogawa Y, Kirisawa R, Kawakami Y.
Establishment of B-cell lines from tumor of enzootic bovine leukosis
Leuk Res . 1986
1986
10(6):689-95
PubMed ID: 3012214
DOI: 10.1016/0145-2126(86)90274-2
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