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Cell No. : Cell Name
RCB0417 : KU-1  update : 2022/03/14
CommentBovine B cell leukemia cell line. It is described in the paper that bovine leukaemia virus (BLV) particles and BLV antigen are both negative. In relation to the production of virus, RIKEN Cell Bank has not confirmed it.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Aida, Yoko
Originator Koyama, Hiroyuki
Year of deposit 1989
Animal bovine < Mammals
Gender Female
Age at sampling 4 years
Tissue blood
Classification cancer
Year of origin 1982
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_4711
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 20% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 1

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1789  Koyama H, Imamura Y, Yoshikawa H, Kajikawa O, Itohara S, Mizuno Y, Yoshikawa T, Saito H.  Establishment of a Continuous Cell Line Derived from Leukaemic Cattle  J Vet Med B  1986  33:586-596  PubMed ID: 3026121   DOI: 10.1111/j.1439-0450.1986.tb00073.x

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User's Publication
15541  Susumu Muroya, Ikuyo Nakajima, Koichi Chikuni  Bovine Skeletal Muscle Cells Predominantly Express a Vascular Cell Adhesion Molecule-1 Seven-Ig Domain Splice Form  Zoological Science  2001  18:797-805    DOI: 10.2108/zsj.18.797

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