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Cell No. : Cell Name
RCB0414 : NIAS-MaBr-93  update : 2024/01/17
Commentlarval hemocyte
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi, J. & Shozawa, A.
Year of deposit 1989
Animal _armyworm < Insect
Strain name cabbage armyworm
Genus Mamestra
Species brassicae (Linnaeus)
Tissue blood, larval
Classification other
Year of origin 1982
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z125
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium MM (or Schneider's) + 3% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP, G6PD, PepB
Reference information Reference 2
User's Publication 0


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Reference
1344  Jun MITSUHASHI  Differences in Amino Acid Metabolism among Cell Lines Derived from Cabbage Armyworm, Mamestra brassicae : Lepidoptera : Noctuidae  Appl Ent Zool  1987  22:533-536   
1043  Mitsuhashi J, Shozawa A.  Continuous Cell Lines from Larval Hemocytes of the Cabbage Armyworm, Mamestra brassiae: (continuous cell line/hemocytes/Mamestra brassicae)  Dev Growth Differ  1985  27(5):599-60  PubMed ID: 37280827   DOI: 10.1111/j.1440-169X.1985.00599.x

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