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Cell No. : Cell Name
RCB0413 : NIAS-MB-32  update : 2022/03/14
CommentShow finely branched cytoplasmic processes. Application consideration
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi, Jun
Year of deposit 1989
Animal _armyworm < Insect
Strain name cabbage armyworm
Genus Mamestra
Species brassicae (Linnaeus)
Tissue pupal ovary
Classification other
Year of origin 1974
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z123
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium MM (or Schneider's) + 3% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 6
User's Publication 1


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Reference
1092  MITSUHASHI J.  Simplification of media and utilization of sugars by insect cells in cultures.  Invertebrate and Fish Tissue Culture  1988  15-18   
1344  Jun MITSUHASHI  Differences in Amino Acid Metabolism among Cell Lines Derived from Cabbage Armyworm, Mamestra brassicae : Lepidoptera : Noctuidae  Appl Ent Zool  1987  22:533-536   
1343  Mitsuhashi. Jun  Total Fatty Acids of Some Insect Cell lines  Appl Ent Zool  1985  20:8-12   
1345  Shigeru KIMURA, Jun MITSUHASHI  Preliminary Notes on Glycosidases in Two Cell Lines Derived from the Ovary of the Cabbage Armyworm, Mamestra brassicae (Lepidoptera : Noctuidae)  Appl Ent Zool  1983  18:561-563   
1346  Jun MITSUHASHI  Continuous Cultures of Insect Cell Lines in Media Free of Sera  Appl Ent Zool  1982  17:575-581   
1042  Mitsuhashi J.  ESTABLISHMENT AND CHARACTERIZATION OF CONTINUOUS CELL LINES FROM PUPAL OVARIES OF THE CABBAGE ARMYWORM, MAMESTRA BRASSICAE (LEPIDOPTERA, NOCTUIDAE).  Dev Growth Differ  1977  19(4):337-344  PubMed ID: 37281049  

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User's Publication
14829  Yamamoto Y, Kajiura H, Nishibu T, Fujiyama K.  Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression  J Biosci Bioeng  2022  134(5):432-440  PubMed ID: 36163133   DOI: 10.1016/j.jbiosc.2022.07.010



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