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Cell No. : Cell Name
RCB0412 : NIAS-PX-64  update : 2024/01/17
CommentPupal ovary.
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi, Jun
Year of deposit 1989
Animal _butterfly < Insect
Strain name swallow t.
Genus Papilio
Species xuthus Linne
Tissue pupal ovary
Classification other
Year of origin 1970
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z134
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium MM (or Schneider's) + 3% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
Temperature 28 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
1183  Inoue, Hajime and Mitsuhashi, Jun  Characterization of a new Continuous Cell Line from Silkworm (Bombyx mori) Embryos.  Appl Ent Zool  1988  23:43326    DOI: 10.1303/aez.23.8
1092  MITSUHASHI J.  Simplification of media and utilization of sugars by insect cells in cultures.  Invertebrate and Fish Tissue Culture  1988  15-18   
1094  Jun MITSUHASHI  Establishment of Cell Lines from the Pupal Ovaries of the Swallow Tail, Papilio xuthus LINNE : Lepidoptera : Papilionidae  Applied Entomology and Zoology  1973  8:64-72    DOI: 10.1303/aez.8.64

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