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Cell No. : Cell Name
RCB0410 : MEN1RGB  update : 2022/03/14
CommentFibroblasts from multiple endocrine neoplasia
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Cell-Medicine, Inc.
Headquater
2-1-6 Sengen, Tsukuba, Ibaraki 305-0047 Japan
Dr.OHNO Tadao
Fax. +81-29-828-5592



English
Address
The University of Tokushima
Institute for Genome Research
3-18-15, Kuramoto-Cho, Tokushima-Shi, Tokushima-Ken, 770-8503, Japan
Dr.ITAKURA Mitsuo
Fax. +81-88-631-9476
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Itakura, M. & Ohno, T.
Originator Ohno, Tadao & Kurita, Kaoru
Year of deposit 1989
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 62 years
Tissue adrenal cortex
Genetics multiple endocrine neoplasia, type II
Classification semi-normal
Year of origin 1989
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_H212
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 44-94(50) : 44(2),45(4),46(37),47(3),74(1),92(2)/
STR(human) OK
Reference information Reference 0
User's Publication 0


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